Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification and properties of DNA gyrase from Vibrio cholerae.

Literature DB >> 1662488

Purification and properties of DNA gyrase from Vibrio cholerae.

Abstract

The DNA gyrase was purified from Vibrio cholerae strain 569B. It appeared to be composed of two subunits of Mr 120,000 and 97,000 and had a great deal of similarity to the Escherichia coli gyrase. Unlike the E. coli enzyme, however, it could neither relax supercoiled DNA nor induce a cleavage of double-stranded DNA, under experimental conditions in which E. coli gyrase could do so.

Entities: Species

Mesh：

Substances：

Year: 1991 PMID： 1662488 PMCID： PMC1130524 DOI： 10.1042/bj2800797

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

21 in total

1. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.

Authors: A Sugino; C L Peebles; K N Kreuzer; N R Cozzarelli
Journal: Proc Natl Acad Sci U S A Date: 1977-11 Impact factor: 11.205

2. DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Authors: M Gellert; K Mizuuchi; M H O'Dea; H A Nash
Journal: Proc Natl Acad Sci U S A Date: 1976-11 Impact factor: 11.205

3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Deoxyribonucleic acid gyrase-deoxyribonucleic acid complex containing 140 base pairs of deoxyribonucleic acid and an alpha 2 beta 2 protein core.

Authors: L Klevan; J C Wang
Journal: Biochemistry Date: 1980-11-11 Impact factor: 3.162

1. DNA gyrase from the albicidin producer Xanthomonas albilineans has multiple-antibiotic-resistance and unusual enzymatic properties.

Authors: Saeed M Hashimi; Guozhong Huang; Anthony Maxwell; Robert G Birch
Journal: Antimicrob Agents Chemother Date: 2008-02-11 Impact factor: 5.191

1 in total

Purification and properties of DNA gyrase from Vibrio cholerae.

1. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.

2. DNA gyrase: an enzyme that introduces superhelical turns into DNA.

3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Deoxyribonucleic acid gyrase-deoxyribonucleic acid complex containing 140 base pairs of deoxyribonucleic acid and an alpha 2 beta 2 protein core.

6. DNA gyrase subunit stoichiometry and the covalent attachment of subunit A to DNA during DNA cleavage.

7. Metabolic reactions responsible for glucose stimulation of alkaline phosphatase in Vibrio cholerae.

8. DNA gyrase: subunit structure and ATPase activity of the purified enzyme.

9. Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.

10. Micrococcus luteus DNA gyrase: active components and a model for its supercoiling of DNA.

1. DNA gyrase from the albicidin producer Xanthomonas albilineans has multiple-antibiotic-resistance and unusual enzymatic properties.