Literature DB >> 6276358

Partial purification and cleavage specificity of a site-specific endonuclease, SciNI, isolated from Spiroplasma citri.

M A Stephens.   

Abstract

A site-specific endonuclease, SciNI, has been partially purified from the plant pathogen Spiroplasma citri. The enzyme recognizes the sequence 5'-G-C-G-C-3' and cleaves between the first G and C. 3'-C-G-C-G-5' SciNI is an isoschizomer of HhaI, but generates DNA fragments with 5' rather than 3' single-stranded protrusions.

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Year:  1982        PMID: 6276358      PMCID: PMC216535          DOI: 10.1128/jb.149.2.508-514.1982

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  14 in total

1.  The genes for cytoplasmic ribosomal ribonucleic Acid in higher plants.

Authors:  N S Scott; J Ingle
Journal:  Plant Physiol       Date:  1973-04       Impact factor: 8.340

2.  Spiroplasmavirus citri 3: propagation, purification, proteins, and nucleic acid.

Authors:  R M Cole; W O Mitchell; C F Garon
Journal:  Science       Date:  1977-12-23       Impact factor: 47.728

3.  A simple, general procedure for purifying restriction endonucleases.

Authors:  T A Bickle; V Pirrotta; R Imber
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

4.  pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

Authors:  J G Sutcliffe
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

5.  Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes.

Authors:  H O Smith; D Nathans
Journal:  J Mol Biol       Date:  1973-12-15       Impact factor: 5.469

6.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

7.  The phylogeny of prokaryotes.

Authors:  G E Fox; E Stackebrandt; R B Hespell; J Gibson; J Maniloff; T A Dyer; R S Wolfe; W E Balch; R S Tanner; L J Magrum; L B Zablen; R Blakemore; R Gupta; L Bonen; B J Lewis; D A Stahl; K R Luehrsen; K N Chen; C R Woese
Journal:  Science       Date:  1980-07-25       Impact factor: 47.728

8.  A general method for defining restriction enzyme cleavage and recognition sites.

Authors:  N L Brown; M Smith
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

9.  Site specific endonuclease from Fusobacterium nucleatum.

Authors:  A C Lui; B C McBride; G F Vovis; M Smith
Journal:  Nucleic Acids Res       Date:  1979-01       Impact factor: 16.971

10.  A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum.

Authors:  D J McConnell; D G Searcy; J G Sutcliffe
Journal:  Nucleic Acids Res       Date:  1978-06       Impact factor: 16.971

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  10 in total

1.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

2.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1989       Impact factor: 16.971

3.  Identification of a novel site specific endonuclease produced by Mycoplasma fermentans: discovery while characterizing DNA binding proteins in T lymphocyte cell lines.

Authors:  N F Halden; J B Wolf; W J Leonard
Journal:  Nucleic Acids Res       Date:  1989-05-11       Impact factor: 16.971

4.  Restriction and modification enzymes and their recognition sequences.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1985       Impact factor: 16.971

5.  Identification and preliminary characterization of external membrane-bound nuclease activities in Mycoplasma pulmonis.

Authors:  F C Minion; J D Goguen
Journal:  Infect Immun       Date:  1986-01       Impact factor: 3.441

6.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1988       Impact factor: 16.971

Review 7.  Molecular biology and genetics of mycoplasmas (Mollicutes).

Authors:  S Razin
Journal:  Microbiol Rev       Date:  1985-12

8.  Properties of the nucleases of mollicutes.

Authors:  J D Pollack; P J Hoffmann
Journal:  J Bacteriol       Date:  1982-10       Impact factor: 3.490

9.  Purification and characterization of Mycoplasma penetrans Ca2+/Mg2+-dependent endonuclease.

Authors:  M Bendjennat; A Blanchard; M Loutfi; L Montagnier; E Bahraoui
Journal:  J Bacteriol       Date:  1997-04       Impact factor: 3.490

10.  Integrative and free Spiroplasma citri oriC plasmids: expression of the Spiroplasma phoeniceum spiralin in Spiroplasma citri.

Authors:  J Renaudin; A Marais; E Verdin; S Duret; X Foissac; F Laigret; J M Bové
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

  10 in total

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