Literature DB >> 2786191

Identification of a novel site specific endonuclease produced by Mycoplasma fermentans: discovery while characterizing DNA binding proteins in T lymphocyte cell lines.

N F Halden1, J B Wolf, W J Leonard.   

Abstract

We have discovered a new restriction endonuclease, MfeI, in nuclear extracts from T cells contaminated with Mycoplasma fermentans. This endonuclease was identified while studying proteins binding to the interleukin-2 receptor alpha chain gene promoter. MfeI cuts at the recognition sequence C'AATTG generating EcoRI compatible cohesive ends. Potential applications are discussed.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2786191      PMCID： PMC317791          DOI： 10.1093/nar/17.9.3491

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.

Authors:  D J Galas; A Schmitz
Journal:  Nucleic Acids Res       Date:  1978-09       Impact factor: 16.971

2.  Chromosomal subunits in active genes have an altered conformation.

Authors:  H Weintraub; M Groudine
Journal:  Science       Date:  1976-09-03       Impact factor: 47.728

3.  Novel method for identifying sequence-specific DNA-binding proteins.

Authors:  D Levens; P M Howley
Journal:  Mol Cell Biol       Date:  1985-09       Impact factor: 4.272

4.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1988       Impact factor: 16.971

5.  Partial purification and cleavage specificity of a site-specific endonuclease, SciNI, isolated from Spiroplasma citri.

Authors:  M A Stephens
Journal:  J Bacteriol       Date:  1982-02       Impact factor: 3.490

6.  Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.

Authors:  C Wu
Journal:  Nature       Date:  1984 May 17-23       Impact factor: 49.962

7.  Regulation of the human interleukin-2 receptor alpha chain promoter: activation of a nonfunctional promoter by the transactivator gene of HTLV-I.

Authors:  S L Cross; M B Feinberg; J B Wolf; N J Holbrook; F Wong-Staal; W J Leonard
Journal:  Cell       Date:  1987-04-10       Impact factor: 41.582

8.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

9.  Structure of the human interleukin-2 receptor gene.

Authors:  W J Leonard; J M Depper; M Kanehisa; M Krönke; N J Peffer; P B Svetlik; M Sullivan; W C Greene
Journal:  Science       Date:  1985-11-08       Impact factor: 47.728
  9 in total
  3 in total

1.  Genotypic and phenotypic analysis of Mycoplasma fermentans strains isolated from different host tissues.

Authors:  L Campo; P Larocque; T La Malfa; W D Blackburn; H L Watson
Journal:  J Clin Microbiol       Date:  1998-05       Impact factor: 5.948

2.  Mycoplasma hyopneumoniae mhp379 is a Ca2+-dependent, sugar-nonspecific exonuclease exposed on the cell surface.

Authors:  Jonathan A Schmidt; Glenn F Browning; Philip F Markham
Journal:  J Bacteriol       Date:  2007-02-16       Impact factor: 3.490

3.  Purification and characterization of Mycoplasma penetrans Ca2+/Mg2+-dependent endonuclease.

Authors:  M Bendjennat; A Blanchard; M Loutfi; L Montagnier; E Bahraoui
Journal:  J Bacteriol       Date:  1997-04       Impact factor: 3.490
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.