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Literature DB >> 673834

A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum.

D J McConnell, D G Searcy, J G Sutcliffe.

Abstract

A type II restriction enzyme (Tha I) has been isolated from the thermophilic mycoplasma Thermoplasma acidophilum. A new method of general application was used to determine the DNA sequence cleaved by the enzyme. Tha I cuts DNA in the centre of the sequence CGCG. Single-stranded DNA is not a substrate. Tha I does not cut T. acidophilum DNA which is presumably modified. This is the first description of a restriction enzyme from a mycoplasma. Because Tha I is easily prepared in large amounts of approximately 10(5) units per gram of cells, it will be a valuable addition to the battery of restriction enzymes used in studies of DNA sequences. It is active at high temperatures and may therefore be useful for special purposes requiring more extreme conditions.

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Year: 1978 PMID： 673834 PMCID： PMC342121 DOI： 10.1093/nar/5.6.1729

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

1. Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus.

Authors: D Kleid; Z Humayun; A Jeffrey; M Ptashne
Journal: Proc Natl Acad Sci U S A Date: 1976-02 Impact factor: 11.205

2. A second specific endonuclease from Haemophilus aegyptius.

Authors: R J Roberts; J B Breitmeyer; N F Tabachnik; P A Myers
Journal: J Mol Biol Date: 1975-01-05 Impact factor: 5.469

3. Histone-like protein in the prokaryote Thermoplasma acidophilum.

Authors: D G Searcy
Journal: Biochim Biophys Acta Date: 1975-07-23

4. Nucleotide sequence of bacteriophage phi X174 DNA.

Authors: F Sanger; G M Air; B G Barrell; N L Brown; A R Coulson; C A Fiddes; C A Hutchison; P M Slocombe; M Smith
Journal: Nature Date: 1977-02-24 Impact factor: 49.962

Review 5. Mycoplasma viruses: isolation, physicochemical, and biological properties.

Authors: R N Gourlay
Journal: CRC Crit Rev Microbiol Date: 1974

6. Endonuclease R. Hind fragments of T7 DNA.

Authors: P Humphries; R L Gordon; D J McConnell; P Connolly
Journal: Virology Date: 1974-03 Impact factor: 3.616

7. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

8. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.

Authors: T Tanaka; B Weisblum
Journal: J Bacteriol Date: 1975-01 Impact factor: 3.490

Review 9. Restriction endonucleases.

Authors: R J Roberts
Journal: CRC Crit Rev Biochem Date: 1976-11

10. Acidic hydrolysis of deoxycytidine and deoxyuridine derivatives. The general mechanism of deoxyribonucleoside hydrolysis.

Authors: R Shapiro; M Danzig
Journal: Biochemistry Date: 1972-01-04 Impact factor: 3.162

38 in total

A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum.

1. Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus.

2. A second specific endonuclease from Haemophilus aegyptius.

3. Histone-like protein in the prokaryote Thermoplasma acidophilum.

4. Nucleotide sequence of bacteriophage phi X174 DNA.

Review 5. Mycoplasma viruses: isolation, physicochemical, and biological properties.

6. Endonuclease R. Hind fragments of T7 DNA.

7. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

8. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.

Review 9. Restriction endonucleases.

10. Acidic hydrolysis of deoxycytidine and deoxyuridine derivatives. The general mechanism of deoxyribonucleoside hydrolysis.

1. Promoter mutations in the transfer RNA gene tyrT of Escherichia coli.

2. Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicus.

3. Evidence for salt-associated restriction pattern modifications in the archaeobacterium Haloferax mediterranei.

4. Restriction enzymes and their isoschizomers.

5. Restriction enzymes and their isoschizomers.

Review 6. Archaeal extrachromosomal genetic elements.

Review 7. Methanogens and the diversity of archaebacteria.

8. BepI restriction endonuclease, a new isoschisomer of FnuDII.

9. Characterization of a site-specific restriction endonuclease from Rhodopseudomonas sphaeroides.

10. Evidence for two restriction-modification systems in Halobacterium cutirubrum.