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Literature DB >> 909783

A simple, general procedure for purifying restriction endonucleases.

Abstract

A simple, general method for purifying restriction endonucleases is described. The method employs precipitation of nucleic acids from crude extracts with polyethyleneimine followed by affinity chromatography on columns of heparin covalently linked to agarose. Most of the sixteen enzymes tested could be purified to a degree sufficient for DNA sequencing work by this method sometimes supplemented by at most one step of ion exchange chromatography.

Entities: Chemical

Mesh：

Substances：

Year: 1977 PMID： 909783 PMCID： PMC342592 DOI： 10.1093/nar/4.8.2561

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

11 in total

1. Rapid isolation of highly active RNA polymerase from Escherichia coli and its subunits by matrix-bound heparin.

Authors: H Sternbach; R Engelhardt; A G Lezius
Journal: Eur J Biochem Date: 1975-12-01

2. Multiple steps in DNA recognition by restriction endonuclease from E. coli K.

Authors: R Yuan; T A Bickle; W Ebbers; C Brack
Journal: Nature Date: 1975-08-14 Impact factor: 49.962

3. Agarose slab-gel electrophoresis equipment.

Authors: B Sugden; B De Troy; R J Roberts; J Sambrook
Journal: Anal Biochem Date: 1975-09 Impact factor: 3.365

4. A rapid procedure for purification of EcoRI endonuclease.

Authors: J Sümegi; D Breedveld; P Hossenlopp; P Chambon
Journal: Biochem Biophys Res Commun Date: 1977-05-09 Impact factor: 3.575

5. A thermostable sequence-specific endonuclease from Thermus aquaticus.

Authors: S Sato; C A Hutchinson; J I Harris
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

6. The purification of restriction endonuclease EcoRI by precipitation involving polyethyleneimine.

Authors: A H Bingham; A F Sharman; T Atkinson
Journal: FEBS Lett Date: 1977-04-15 Impact factor: 4.124

7. A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.

Authors: W Zillig; K Zechel; H J Halbwachs
Journal: Hoppe Seylers Z Physiol Chem Date: 1970-02

8. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.

Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1968-02 Impact factor: 3.162

9. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.

Authors: R R Burgess; J J Jendrisak
Journal: Biochemistry Date: 1975-10-21 Impact factor: 3.162

Review 10. Restriction endonucleases.

Authors: R J Roberts
Journal: CRC Crit Rev Biochem Date: 1976-11

63 in total

1. Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr region of the Escherichia coli chromosome.

Authors: R P Gunsalus; G Zurawski; C Yanofsky
Journal: J Bacteriol Date: 1979-10 Impact factor: 3.490

2. Deletion mutants of polyoma virus defining a nonessential region between the origin of replication and the initiation codon for early proteins.

Authors: M M Bendig; W R Folk
Journal: J Virol Date: 1979-11 Impact factor: 5.103

3. Inverted terminal repeats in rabbit poxvirus and vaccinia virus DNA.

Authors: R Wittek; A Menna; H K Müller; D Schümperli; P G Boseley; R Wyler
Journal: J Virol Date: 1978-10 Impact factor: 5.103

4. Sigma-like activity from mustard (Sinapis alba L.) chloroplasts conferring DNA-binding and transcription specificity to E. coli core RNA polymerase.

Authors: S Bülow; G Link
Journal: Plant Mol Biol Date: 1988-07 Impact factor: 4.076