Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification and characterization of a kanamycin nucleotidyltransferase from plasmid pUB110-carrying cells of Bacillus subtilis.

Literature DB >> 6245061

Purification and characterization of a kanamycin nucleotidyltransferase from plasmid pUB110-carrying cells of Bacillus subtilis.

Abstract

The nucleotidyltransferase encoded by plasmid pUB110 was purified to greater than 95% purity with a 33% yield. The enzyme is a monomeric protein with a molecular weight of 34,000. The optimum pH for activity is 5, and the optimum MgCl2 concentration for activity is 18 mM. The enzyme, which is synthesized constitutively, is stable for several weeks at 4 degrees C. This enzyme would appear to be a good model gene product for the development of a pUB110 deoxyribonucleic acid-dependent in vitro protein-synthesizing system from Bacillus subtilis.

Entities: Chemical Species

Mesh：

Substances：

Year: 1980 PMID： 6245061 PMCID： PMC293806 DOI： 10.1128/jb.141.3.1178-1182.1980

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

18 in total

Review 1. Role of ribonucleic acid polymerase in gene selection in procaryotes.

Authors: R H Doi
Journal: Bacteriol Rev Date: 1977-09

2. Reconstitution studies show that rifampicin resistance is determined by the largest polypeptide of Bacillus subtilis RNA polymerase.

Authors: S M Halling; K C Burtis; R H Doi
Journal: J Biol Chem Date: 1977-12-25 Impact factor: 5.157

3. Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor.

Authors: R Benveniste; J Davies
Journal: Biochemistry Date: 1971-05-11 Impact factor: 3.162

4. Aminoglycoside antibiotics: inactivation by phosphorylation in Escherichia coli carrying R factors.

Authors: B Ozanne; R Benveniste; D Tipper; J Davies
Journal: J Bacteriol Date: 1969-11 Impact factor: 3.490

5. Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.

Authors: J L Hedrick; A J Smith
Journal: Arch Biochem Biophys Date: 1968-07 Impact factor: 4.013

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. Repair deficiency, mutator activity, and thermal prophage inducibility in dna-8132 strains of Bacillus subtilis.

Authors: Y Sadaie; K Narui
Journal: J Bacteriol Date: 1976-06 Impact factor: 3.490

8. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

Authors: J J Sedmak; S E Grossberg
Journal: Anal Biochem Date: 1977-05-01 Impact factor: 3.365

9. New plasmid-mediated nucleotidylation of aminoglycoside antibiotics in Staphlococcus aureus.

Authors: F Le Goffic; A Martel; M L Capmau; B Baca; P Goebel; H Chardon; C J Soussy; J Duval; D H Bouanchaud
Journal: Antimicrob Agents Chemother Date: 1976-08 Impact factor: 5.191

10. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.

Authors: S D Ehrlich
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

19 in total

1. Mutations in bglY, the structural gene for the DNA-binding protein H1 of Escherichia coli, increase the expression of the kanamycin resistance gene carried by plasmid pGR71.

Authors: P Bertin; P Lejeune; C Colson; A Danchin
Journal: Mol Gen Genet Date: 1992-05

2. Identification and nucleotide sequence of the promoter region of the Bacillus subtilis gluconate operon.

Authors: Y Fujita; T Fujita
Journal: Nucleic Acids Res Date: 1986-02-11 Impact factor: 16.971

3. Does single-amino-acid replacement work in favor of or against improvement of the thermostability of immobilized enzyme?

Authors: J Koizumi; M Zhang; T Imanaka; S Aiba
Journal: Appl Environ Microbiol Date: 1990-11 Impact factor: 4.792

Purification and characterization of a kanamycin nucleotidyltransferase from plasmid pUB110-carrying cells of Bacillus subtilis.

Review 1. Role of ribonucleic acid polymerase in gene selection in procaryotes.

2. Reconstitution studies show that rifampicin resistance is determined by the largest polypeptide of Bacillus subtilis RNA polymerase.

3. Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor.

4. Aminoglycoside antibiotics: inactivation by phosphorylation in Escherichia coli carrying R factors.

5. Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Repair deficiency, mutator activity, and thermal prophage inducibility in dna-8132 strains of Bacillus subtilis.

8. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

9. New plasmid-mediated nucleotidylation of aminoglycoside antibiotics in Staphlococcus aureus.

10. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.

1. Mutations in bglY, the structural gene for the DNA-binding protein H1 of Escherichia coli, increase the expression of the kanamycin resistance gene carried by plasmid pGR71.

2. Identification and nucleotide sequence of the promoter region of the Bacillus subtilis gluconate operon.

3. Does single-amino-acid replacement work in favor of or against improvement of the thermostability of immobilized enzyme?

Review 4. Antimicrobial resistance of Staphylococcus aureus: genetic basis.

5. Single amino acid replacements affecting the thermostability of kanamycin nucleotidyltransferase.

6. Cloning restriction fragments that promote expression of a gene in Bacillus subtilis.

7. Bacteriophage phi 29 proteins required for in vitro DNA-gp3 packaging.

8. Regulatory regions that control expression of two chloramphenicol-inducible cat genes cloned in Bacillus subtilis.

9. Cloning of an early sporulation gene in Bacillus subtilis.

10. Inhibition of growth of Bacillus subtilis by recombinant plasmid pCED3.