Literature DB >> 6787015

Cloning restriction fragments that promote expression of a gene in Bacillus subtilis.

D M Williams, E J Duvall, P S Lovett.   

Abstract

Plasmid pPL603 (3.1 megadaltons) specifies neomycin resistance in Bacillus subtilis and contains a structural gene for chloramphenicol acetyltransferase. Cells harboring the plasmid cannot grow on solid media containing 10 microgram of chloramphenicol per ml. Cloning EcoRI (or EcoRI)-generated fragments of deoxyribonucleic acid from several sources into the single EcoRI site in plasmid pPL603, with subsequent selection of transformants of media containing 10 micrograms of chloramphenicol per ml, permits the identification of restriction fragments that promote expression of the chloramphenicol acetyltransferase gene.

Entities:  

Mesh：

Substances：

Year:  1981        PMID： 6787015      PMCID： PMC216974          DOI： 10.1128/jb.146.3.1162-1165.1981

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.

Authors:  W V Shaw
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

2.  Correlated genetic and EcoRI cleavage map of Bacillus subtilis bacteriophage phi105 DNA.

Authors:  B M Scher; M F Law; A J Garro
Journal:  J Virol       Date:  1978-10       Impact factor: 5.103

3.  Specificity of substrate recognition by the EcoRI restriction endonuclease.

Authors:  B Polisky; P Greene; D E Garfin; B J McCarthy; H M Goodman; H W Boyer
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.

Authors:  M J Casadaban; J Chou; S N Cohen
Journal:  J Bacteriol       Date:  1980-08       Impact factor: 3.490

Review 6.  Bacteriophages of Bacillus subtilis.

Authors:  H E Hemphill; H R Whiteley
Journal:  Bacteriol Rev       Date:  1975-09

7.  Recombination between compatible plasmids containing homologous segments requires the Bacillus subtilis recE gene product.

Authors:  K M Keggins; E J Duvall; P S Lovett
Journal:  J Bacteriol       Date:  1978-05       Impact factor: 3.490

8.  Bacillus pumilus plasmid pPL10: properties and insertion into Bacillus subtilis 168 by transformation.

Authors:  P S Lovett; E J Duvall; K M Keggins
Journal:  J Bacteriol       Date:  1976-08       Impact factor: 3.490

9.  Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.

Authors:  T J Gryczan; S Contente; D Dubnau
Journal:  J Bacteriol       Date:  1978-04       Impact factor: 3.490

10.  Development of competence in the Bacillus subtilis transformation system.

Authors:  K F Bott; G A Wilson
Journal:  J Bacteriol       Date:  1967-09       Impact factor: 3.490
View more
  60 in total

1.  Organization and regulation of the Bacillus subtilis odhAB operon, which encodes two of the subenzymes of the 2-oxoglutarate dehydrogenase complex.

Authors:  O Resnekov; L Melin; P Carlsson; M Mannerlöv; A von Gabain; L Hederstedt
Journal:  Mol Gen Genet       Date:  1992-08

2.  Cloning and expression in Escherichia coli of chromosomal mercury resistance genes from a Bacillus sp.

Authors:  Y Wang; I Mahler; H S Levinson; H O Halvorson
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

3.  Relationship between the Clostridium perfringens catQ gene product and chloramphenicol acetyltransferases from other bacteria.

Authors:  T L Bannam; J I Rood
Journal:  Antimicrob Agents Chemother       Date:  1991-03       Impact factor: 5.191

4.  Ribosome hopping and translational frameshifting are inadequate alternatives to translational attenuation in cat-86 regulation.

Authors:  E J Rogers; N P Ambulos; P S Lovett
Journal:  J Bacteriol       Date:  1991-12       Impact factor: 3.490

5.  Identification and nucleotide sequence of the promoter region of the Bacillus subtilis gluconate operon.

Authors:  Y Fujita; T Fujita
Journal:  Nucleic Acids Res       Date:  1986-02-11       Impact factor: 16.971

6.  Selective expression of a plasmid cat gene at a late stage of Bacillus subtilis sporulation.

Authors:  S Mongkolsuk; P S Lovett
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

7.  Mutational analysis of cat-86 gene expression controlled by lactococcal promoters in Lactococcus lactis subsp. lactis and Escherichia coli.

Authors:  B Bojovic; G Djordjevic; A Banina; L Topisirovic
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

8.  Use of chromosomal integration in the establishment and expression of blaZ, a Staphylococcus aureus beta-lactamase gene, in Bacillus subtilis.

Authors:  C W Saunders; B J Schmidt; M S Mirot; L D Thompson; M S Guyer
Journal:  J Bacteriol       Date:  1984-03       Impact factor: 3.490

9.  Regulatory regions that control expression of two chloramphenicol-inducible cat genes cloned in Bacillus subtilis.

Authors:  E J Duvall; D M Williams; S Mongkolsuk; P S Lovett
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490

10.  Promoter-probe plasmid for Bacillus subtilis.

Authors:  C E Donnelly; A L Sonenshein
Journal:  J Bacteriol       Date:  1984-03       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.