Literature DB >> 2176451

Does single-amino-acid replacement work in favor of or against improvement of the thermostability of immobilized enzyme?

J Koizumi1, M Zhang, T Imanaka, S Aiba.   

Abstract

Thermostabilities of kanamycin nucleotidyltransferase and of its mutants that became thermostable, in the free state, because of single-amino-acid replacements were studied after immobilization of the enzymes on cyanogen bromide-activated Sephadex G-200 particles. Lys in place of Gln at position 102 decreased the thermostability of the immobilized enzyme, whereas replacement with other amino acids enhanced it.

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Year:  1990        PMID： 2176451      PMCID： PMC185035          DOI： 10.1128/aem.56.11.3612-3614.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  11 in total

1.  The principles of enzyme stabilization. I. Increase in thermostability of enzymes covalently bound to a complementary surface of a polymer support in a multipoint fashion.

Authors:  K Martinek; A M Klibanov; V S Goldmacher; I V Berezin
Journal:  Biochim Biophys Acta       Date:  1977-11-23

2.  Single amino acid replacements affecting the thermostability of kanamycin nucleotidyltransferase.

Authors:  M Matsumura; S Kataoka; S Aiba
Journal:  Mol Gen Genet       Date:  1986-08

3.  Cumulative effect of intragenic amino-acid replacements on the thermostability of a protein.

Authors:  M Matsumura; S Yasumura; S Aiba
Journal:  Nature       Date:  1986 Sep 25-Oct 1       Impact factor: 49.962

4.  A new way of enhancing the thermostability of proteases.

Authors:  T Imanaka; M Shibazaki; M Takagi
Journal:  Nature       Date:  1986 Dec 18-31       Impact factor: 49.962

5.  Purification and characterization of a kanamycin nucleotidyltransferase from plasmid pUB110-carrying cells of Bacillus subtilis.

Authors:  Y Sadaie; K C Burtis; R H Doi
Journal:  J Bacteriol       Date:  1980-03       Impact factor: 3.490

6.  Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.

Authors:  H Liao; T McKenzie; R Hageman
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

7.  Temperature-dependent plasmid integration into and excision from the chromosome of Bacillus stearothermophilus.

Authors:  J Koizumi; Z Min; T Imanaka; S Aiba
Journal:  J Gen Microbiol       Date:  1986-07

8.  An immobilized three-enzyme system: a model for microenvironmental compartmentation in mitochondria.

Authors:  P A Srere; B Mattiasson; K Mosbach
Journal:  Proc Natl Acad Sci U S A       Date:  1973-09       Impact factor: 11.205

9.  Enzymatic and nucleotide sequence studies of a kanamycin-inactivating enzyme encoded by a plasmid from thermophilic bacilli in comparison with that encoded by plasmid pUB110.

Authors:  M Matsumura; Y Katakura; T Imanaka; S Aiba
Journal:  J Bacteriol       Date:  1984-10       Impact factor: 3.490

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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