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Literature DB >> 6191279

A general method to select for M13 clones carrying base pair substitution mutants constructed in vitro.

C Traboni, R Cortese, G Ciliberto, G Cesareni.

Abstract

In this paper we describe a method to select base pair substitution mutants constructed in vitro. The mutagenesis is performed by forcing mistakes during in vitro synthesis from a primer annealed to a single stranded DNA template. The selection is based on the fact that, following transformation, the progeny of the strand elongated in vitro and the template strand have different phenotypes. The method is general and applicable to any DNA segment; the type of base pair substitution and its position can be chosen at will. The combined efficiency of mutagenesis and selection allows for 85% frequency of mutants in all analyzed clones.

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Year: 1983 PMID： 6191279 PMCID： PMC326037 DOI： 10.1093/nar/11.12.4229

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

14 in total

1. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Authors: J Messing; B Gronenborn; B Müller-Hill; P Hans Hopschneider
Journal: Proc Natl Acad Sci U S A Date: 1977-09 Impact factor: 11.205

2. Induction and isolation of mutants in a specific region of gene A of bacteriophage phi chi 174.

Authors: W E Borrias; I J Wilschut; J M Vereijken; P J Weisbeek; G A van Arkel
Journal: Virology Date: 1976-03 Impact factor: 3.616

3. Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments.

Authors: W Kramer; K Schughart; H J Fritz
Journal: Nucleic Acids Res Date: 1982-10-25 Impact factor: 16.971

4. Site-specific mutagenesis by error-directed DNA synthesis.

Authors: R A Zakour; L A Loeb
Journal: Nature Date: 1982-02-25 Impact factor: 49.962

5. Relationship between the two components of the split promoter of eukaryotic tRNA genes.

Authors: G Ciliberto; C Traboni; R Cortese
Journal: Proc Natl Acad Sci U S A Date: 1982-03 Impact factor: 11.205

6. Site-directed mutagenesis of a tRNA gene: base alterations in the coding region affect transcription.

Authors: M S Ciampi; D A Melton; R Cortese
Journal: Proc Natl Acad Sci U S A Date: 1982-03 Impact factor: 11.205

7. Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.

Authors: R Cortese; R Harland; D Melton
Journal: Proc Natl Acad Sci U S A Date: 1980-07 Impact factor: 11.205

8. Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.

Authors: D Shortle; P Grisafi; S J Benkovic; D Botstein
Journal: Proc Natl Acad Sci U S A Date: 1982-03 Impact factor: 11.205

9. Cloning of nematode tRNA genes and their expression in the frog oocyte.

Authors: R Cortese; D Melton; T Tranquilla; J D Smith
Journal: Nucleic Acids Res Date: 1978-12 Impact factor: 16.971

10. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

8 in total

1. U1 small nuclear RNA chimeric ribozymes with substrate specificity for the Rev pre-mRNA of human immunodeficiency virus.

Authors: A Michienzi; S Prislei; I Bozzoni
Journal: Proc Natl Acad Sci U S A Date: 1996-07-09 Impact factor: 11.205

A general method to select for M13 clones carrying base pair substitution mutants constructed in vitro.

1. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

2. Induction and isolation of mutants in a specific region of gene A of bacteriophage phi chi 174.

3. Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments.

4. Site-specific mutagenesis by error-directed DNA synthesis.

5. Relationship between the two components of the split promoter of eukaryotic tRNA genes.

6. Site-directed mutagenesis of a tRNA gene: base alterations in the coding region affect transcription.

7. Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.

8. Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.

9. Cloning of nematode tRNA genes and their expression in the frog oocyte.

10. DNA sequencing with chain-terminating inhibitors.

1. U1 small nuclear RNA chimeric ribozymes with substrate specificity for the Rev pre-mRNA of human immunodeficiency virus.

2. The gapped duplex DNA approach to oligonucleotide-directed mutation construction.

3. Rapid and efficient site-specific mutagenesis without phenotypic selection.

4. Enzymatic techniques for the isolation of random single-base substitutions in vitro at high frequency.

5. Transcription of multimeric tRNA genes.

6. Targeted mutagenesis in vitro: lac repressor mutations generated using AMV reverse transcriptase and dBrUTP.

7. The in vivo mutagenic frequency and specificity of O6-methylguanine in phi X174 replicative form DNA.

8. Structure and expression of the human haptoglobin locus.