Literature DB >> 7001455

Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.

R Cortese, R Harland, D Melton.   

Abstract

The expression of cloned tRNA genes has been studied by injecting single-stranded and double-stranded DNA templates into Xenopus oocyte nuclei. In both forms the genes are faithfully transcribed after injection. Some single-stranded DNA is converted into double-stranded DNA in the oocyte nucleus. This conversion is necessary for the expression of the injected tRNA gene: no tRNA transcription is observed when DNA synthesis is inhibited. We conclude that single-stranded DNA does not serve as a template for faithful transcription of this gene in injected oocytes.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 7001455      PMCID： PMC349787          DOI： 10.1073/pnas.77.7.4147

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.

Authors:  J B Gurdon
Journal:  J Embryol Exp Morphol       Date:  1976-12

2.  In vitro polyoma DNA synthesis: inhibition by 1-beta-d-arabinofuranosyl CTP.

Authors:  T Hunter; B Francke
Journal:  J Virol       Date:  1975-04       Impact factor: 5.103

3.  Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.

Authors:  A H Wyllie; R A Laskey; J Finch; J B Gurdon
Journal:  Dev Biol       Date:  1978-05       Impact factor: 3.582

4.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

5.  High-fidelity transcription of 5S DNA injected into Xenopus oocytes.

Authors:  D D Brown; J B Gurdon
Journal:  Proc Natl Acad Sci U S A       Date:  1977-05       Impact factor: 11.205

6.  Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors:  D B Clewell
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

7.  Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.

Authors:  J B Gurdon; C D Lane; H R Woodland; G Marbaix
Journal:  Nature       Date:  1971-09-17       Impact factor: 49.962

8.  Purified DNAs are transcribed after microinjection into Xenopus oocytes.

Authors:  J E Mertz; J B Gurdon
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

9.  Coupled transcription-translation of DNA injected into Xenopus oocytes.

Authors:  E M De Robertis; J E Mertz
Journal:  Cell       Date:  1977-09       Impact factor: 41.582

10.  Transcription of cloned tRNA gene fragments and subfragments injected into the oocyte nucleus of Xenopus laevis.

Authors:  A Kressmann; S G Clarkson; V Pirrotta; M L Birnstiel
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205
View more
  36 in total

1.  Effect of terminal nonhomologies on homologous recombination in Xenopus laevis oocytes.

Authors:  S Jeong-Yu; D Carroll
Journal:  Mol Cell Biol       Date:  1992-12       Impact factor: 4.272

2.  Processing of targeted psoralen cross-links in Xenopus oocytes.

Authors:  D J Segal; A F Faruqi; P M Glazer; D Carroll
Journal:  Mol Cell Biol       Date:  1997-11       Impact factor: 4.272

3.  Structure of the human alpha 1-acid glycoprotein gene: sequence homology with other human acute phase protein genes.

Authors:  L Dente; G Ciliberto; R Cortese
Journal:  Nucleic Acids Res       Date:  1985-06-11       Impact factor: 16.971

4.  Excision repair of UV-damaged plasmid DNA in Xenopus oocytes is mediated by DNA polymerase alpha (and/or delta).

Authors:  J K Saxena; J B Hays; E J Ackerman
Journal:  Nucleic Acids Res       Date:  1990-12-25       Impact factor: 16.971

5.  Structure of gene and pseudogenes of human apoferritin H.

Authors:  F Costanzo; M Colombo; S Staempfli; C Santoro; M Marone; R Frank; H Delius; R Cortese
Journal:  Nucleic Acids Res       Date:  1986-01-24       Impact factor: 16.971

6.  Cloning of the gene coding for human L apoferritin.

Authors:  C Santoro; M Marone; M Ferrone; F Costanzo; M Colombo; C Minganti; R Cortese; L Silengo
Journal:  Nucleic Acids Res       Date:  1986-04-11       Impact factor: 16.971

7.  Transcription of eucaryotic tRNA1met and 5SRNA genes by RNA polymerase III is blocked by base mismatches in the intragenic control regions.

Authors:  M A Sullivan; W R Folk
Journal:  Nucleic Acids Res       Date:  1987-03-11       Impact factor: 16.971

8.  Point mutations in the 5' ICR and anticodon region of a Drosophila tRNAArg gene decrease in vitro transcription.

Authors:  T S Stewart; D Söll; S Sharp
Journal:  Nucleic Acids Res       Date:  1985-01-25       Impact factor: 16.971

9.  Rapid and apparently error-prone excision repair of nonreplicating UV-irradiated plasmids in Xenopus laevis oocytes.

Authors:  J B Hays; E J Ackerman; Q S Pang
Journal:  Mol Cell Biol       Date:  1990-07       Impact factor: 4.272

10.  Transient gene expression of foreign genes in preheated protoplasts: stimulation of expression of transfected genes lacking heat shock elements.

Authors:  N Zakai; N Ballas; M Hershkovitz; S Broido; R Ram; A Loyter
Journal:  Plant Mol Biol       Date:  1993-03       Impact factor: 4.076
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.