Literature DB >> 6951183

Site-directed mutagenesis of a tRNA gene: base alterations in the coding region affect transcription.

M S Ciampi, D A Melton, R Cortese.   

Abstract

Point mutations have been introduced in vitro in a cloned nematode tRNAPro gene. Four different mutant clones altered in the DNA that codes for tRNAPro have been isolated. Studies on the expression of the mutant genes by microinjection into Xenopus oocyte nuclei reveal that their activities as transcription templates are reduced. The results show that DNA sequences that code for the extra arm and the stem of the T-psi-C-G arm of the tRNAPro have an important role in the initiation of tRNA gene transcription.

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Year:  1982        PMID： 6951183      PMCID： PMC345978          DOI： 10.1073/pnas.79.5.1388

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  24 in total

1.  Promoter mutations in the transfer RNA gene tyrT of Escherichia coli.

Authors:  M L Berman; A Landy
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.

Authors:  J B Gurdon
Journal:  J Embryol Exp Morphol       Date:  1976-12

3.  Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

Authors:  D Shortle; D Nathans
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

4.  Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Authors:  J Messing; B Gronenborn; B Müller-Hill; P Hans Hopschneider
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

5.  A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13.

Authors:  P H Schreier; R Cortese
Journal:  J Mol Biol       Date:  1979-03-25       Impact factor: 5.469

6.  Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites.

Authors:  B Gronenborn; J Messing
Journal:  Nature       Date:  1978-03-23       Impact factor: 49.962

7.  Delimitation of a promoter for RNA polymerase III by means of a functional test.

Authors:  J L Telford; A Kressmann; R A Koski; R Grosschedl; F Müller; S G Clarkson; M L Birnstiel
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

8.  Cloning of nematode tRNA genes and their expression in the frog oocyte.

Authors:  R Cortese; D Melton; T Tranquilla; J D Smith
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

9.  DNA sequence from the histidine operon control region: seven histidine codons in a row.

Authors:  W M Barnes
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  13 in total

1.  Gene size differentially affects the binding of yeast transcription factor tau to two intragenic regions.

Authors:  R E Baker; S Camier; A Sentenac; B D Hall
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

2.  Plant cytosolic tRNAHis possesses an exceptional C54 in the canonical TPsiC loop.

Authors:  K Akama; Y Yukawa; M Sugiura; I Small
Journal:  Nucleic Acids Res       Date:  1998-06-01       Impact factor: 16.971

3.  A general method to select for M13 clones carrying base pair substitution mutants constructed in vitro.

Authors:  C Traboni; R Cortese; G Ciliberto; G Cesareni
Journal:  Nucleic Acids Res       Date:  1983-06-25       Impact factor: 16.971

4.  Enzymatic replacement in vitro of the first anticodon base of yeast tRNAAsp: application to the study of tRNA maturation in vivo, after microinjection into frog oocytes.

Authors:  P Carbon; E Haumont; S De Henau; G Keith; H Grosjean
Journal:  Nucleic Acids Res       Date:  1982-06-25       Impact factor: 16.971

5.  The expression of prokaryotic tRNA genes in frog oocytes.

Authors:  L Bossi; M S Ciampi
Journal:  Nucleic Acids Res       Date:  1983-05-25       Impact factor: 16.971

6.  Functional assay of tRNA molecules transcribed from a purified gene.

Authors:  L Corbo; G Ciliberto; C Traboni; R Santamaria; F Cimino; R Cortese; F Salvatore
Journal:  Nucleic Acids Res       Date:  1982-11-25       Impact factor: 16.971

7.  Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.

Authors:  S Sharp; T Dingermann; J Schaack; J A Sharp; D J Burke; E M DeRobertis; D Söll
Journal:  Nucleic Acids Res       Date:  1983-12-20       Impact factor: 16.971

8.  The 5'-flanking sequence of yeast tRNA(Leu3) genes enhances the rate of transcription from stable pre-initiation complexes.

Authors:  G J Raymond; J D Johnson
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971

9.  TAP1, a yeast gene that activates the expression of a tRNA gene with a defective internal promoter.

Authors:  G Di Segni; B L McConaughy; R A Shapiro; T L Aldrich; B D Hall
Journal:  Mol Cell Biol       Date:  1993-06       Impact factor: 4.272

10.  A new antisense tRNA construct for the genetic treatment of human immunodeficiency virus type 1 infection.

Authors:  M A Biasolo; A Radaelli; L Del Pup; E Franchin; C De Giuli-Morghen; G Palu
Journal:  J Virol       Date:  1996-04       Impact factor: 5.103
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