Literature DB >> 568256

Cloning of chicken lysozyme structural gene sequences synthesized in vitro.

A E Sippel, H Land, W Lindenmaier, M C Nguyen-Huu, T Wurtz, K N Timmis, K Giesecke, G Schütz.   

Abstract

Double-stranded chicken lysozyme cDNA was synthesized from an oviduct mRNA fraction enriched for lysozyme mRNA. The ds-cDNA was inserted into the BamHI site of plasmid pBR322 using chemically synthesized DNA linker molecules containing the BamHI restriction endonuclease cleavage site. After bacterial transformation, colonies carrying lysozyme DNA were identified by hybridization with highly purified lysozyme cDNA. The 555 base pairs long cloned DNA fragment of one recombinant plasmid was isolated and characterized by restriction endonuclease digestion. The DNA sequence of selected parts of the inserted DNA is as predicted from the amino acid sequence of prelysozyme. The sequence data allows the unambiguous location of the coding region within lysozyme mRNA.

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Year:  1978        PMID： 568256      PMCID： PMC342248          DOI： 10.1093/nar/5.9.3275

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  39 in total

1.  THE AMINO ACID SEQUENCE OF EGG WHITE LYSOZYME.

Authors:  R E CANFIELD
Journal:  J Biol Chem       Date:  1963-08       Impact factor: 5.157

2.  ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.

Authors:  C C RICHARDSON; C L SCHILDKRAUT; H V APOSHIAN; A KORNBERG
Journal:  J Biol Chem       Date:  1964-01       Impact factor: 5.157

3.  Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain.

Authors:  S Tonegawa; A M Maxam; R Tizard; O Bernard; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

4.  Amplification and characterization of a beta-globin gene synthesized in vitro.

Authors:  T Maniatis; S G Kee; A Efstratiadis; F C Kafatos
Journal:  Cell       Date:  1976-06       Impact factor: 41.582

5.  A physical map of the DNA regions flanking the rabbit beta-globin gene.

Authors:  A J Jeffreys; R A Flavell
Journal:  Cell       Date:  1977-10       Impact factor: 41.582

6.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

7.  Protein expression in E. coli minicells by recombinant plasmids.

Authors:  R B Meagher; R C Tait; M Betlach; H W Boyer
Journal:  Cell       Date:  1977-03       Impact factor: 41.582

8.  Preferential transcription of the ovalbumin gene in isolated hen oviduct nuclei by RNA polymerase B.

Authors:  M C Nguyen-Huu; A A Sippel; N E Hynes; B Groner; G Schütz
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

9.  The intervening sequence of a mouse beta-globin gene is transcribed within the 15S beta-globin mRNA precursor.

Authors:  S M Tilghman; P J Curtis; D C Tiemeier; P Leder; C Weissmann
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

10.  Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.

Authors:  M P Wickens; G N Buell; R T Schimke
Journal:  J Biol Chem       Date:  1978-04-10       Impact factor: 5.157
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  15 in total

1.  Isolation and partial purification of the major abundant class rat seminal vesicle poly(A+)-messenger RNA.

Authors:  P E Mansson; D B Carter; A B Silverberg; D B Tully; S E Harris
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

2.  Histone acetylation in chicken erythrocytes. Rates of acetylation and evidence that histones in both active and potentially active chromatin are rapidly modified.

Authors:  D E Zhang; D A Nelson
Journal:  Biochem J       Date:  1988-02-15       Impact factor: 3.857

3.  Arrangement of coding and intervening sequences of chicken lysozyme gene.

Authors:  W Lindenmaier; M C Nguyen-Huu; R Lurz; M Stratmann; N Blin; T Wurtz; H J Hauser; A E Sippel; G Schütz
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

4.  Isolation of the lysozyme gene of chicken.

Authors:  P Baldacci; A Royal; B Cami; F Perrin; A Krust; A Garapin; P Kourilsky
Journal:  Nucleic Acids Res       Date:  1979-06-25       Impact factor: 16.971

5.  The beta-globin domain in immature chicken erythrocytes: enhanced solubility is coincident with histone hyperacetylation.

Authors:  D A Nelson; R C Ferris; D E Zhang; C R Ferenz
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

6.  Isolation and characterization of the chicken ovomucoid gene.

Authors:  W Lindenmaier; M C Nguyen-Huu; R Lurz; N Blin; M Stratmann; H Land; S Jeep; A E Sippel; G Schütz
Journal:  Nucleic Acids Res       Date:  1979-11-10       Impact factor: 16.971

7.  Exon cloning: immunoenzymatic identification of exons of the chicken lysozyme gene.

Authors:  U Rüther; M Koenen; A E Sippel; B Müller-Hill
Journal:  Proc Natl Acad Sci U S A       Date:  1982-11       Impact factor: 11.205

8.  DNA organisation in the chicken lysozyme gene region.

Authors:  P Baldacci; A Royal; F Brégégère; J P Abastado; B Cami; F Daniel; P Kourilsky
Journal:  Nucleic Acids Res       Date:  1981-08-11       Impact factor: 16.971

9.  5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.

Authors:  H Land; M Grez; H Hauser; W Lindenmaier; G Schütz
Journal:  Nucleic Acids Res       Date:  1981-05-25       Impact factor: 16.971

10.  [Gene structure and gene expression. Chicken lysozyme gene].

Authors:  G Schütz
Journal:  Naturwissenschaften       Date:  1981-04
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