Literature DB >> 1691209

Expression in Escherichia coli of a dominant immunogen of Trypanosoma cruzi recognized by human chagasic sera.

P C Cotrim1, G S Paranhos, R A Mortara, J Wanderley, A Rassi, M E Camargo, J F da Silveira.   

Abstract

A genomic clone expressing a Trypanosoma cruzi antigen in Escherichia coli was identified using human chagasic sera. Chagasic antibodies affinity purified on extracts of this clone recognized a high-molecular-weight protein expressed in all developmental stages of the parasite life cycle, as well as in various T. cruzi strains. The antigen is associated with the cytoskeleton of the parasite and localizes along the attachment region between the flagellum and the cell body. Antibodies to the recombinant antigen were detected in the sera of 115 chagasic patients from different endemic regions, but not in sera of patients with leishmaniasis, T. rangeli infection, or other parasitic diseases. Our data suggest that the presence of antibodies to this antigen may be specifically associated with Chagas' disease.

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Year:  1990        PMID： 1691209      PMCID： PMC269655          DOI： 10.1128/jcm.28.3.519-524.1990

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Trypanosoma cruzi: polypeptide markers of epimastigotes and trypomastigotes.

Authors:  R Rangel-Aldao; G Comach; O Allende; E Cayama; V Delgado; R Piras; M Piras; D Henriquez; S Negri
Journal:  Mol Biochem Parasitol       Date:  1986-07       Impact factor: 1.759

2.  Schistosoma mansoni: male-biased sex ratios in snails and mice.

Authors:  J D Liberatos
Journal:  Exp Parasitol       Date:  1987-10       Impact factor: 2.011

3.  Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes.

Authors:  J J Lafaille; J Linss; M A Krieger; T Souto-Padrón; W de Souza; S Goldenberg
Journal:  Mol Biochem Parasitol       Date:  1989-06-15       Impact factor: 1.759

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Dissection of Mycobacterium tuberculosis antigens using recombinant DNA.

Authors:  R A Young; B R Bloom; C M Grosskinsky; J Ivanyi; D Thomas; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

6.  Protective immunity and pathology induced by inoculation of mice with different subcellular fractions of Trypanosoma cruzi.

Authors:  A M Ruiz; M Esteva; P Cabeza Meckert; R P Laguens; E L Segura
Journal:  Acta Trop       Date:  1985-12       Impact factor: 3.112

7.  Human and mouse sera recognize the same polypeptide associated with immunological resistance to Trypanosoma cruzi infection.

Authors:  M S Martins; L Hudson; A U Krettli; J R Cançado; Z Brener
Journal:  Clin Exp Immunol       Date:  1985-08       Impact factor: 4.330

8.  Trypanosoma cruzi: immune response in mice immunized with parasite antigens.

Authors:  M E Rottenberg; R L Cardoni; E H deTitto; M Moreno; E L Segura
Journal:  Exp Parasitol       Date:  1988-02       Impact factor: 2.011

9.  Trypanosoma cruzi expresses diverse repetitive protein antigens.

Authors:  D F Hoft; K S Kim; K Otsu; D R Moser; W J Yost; J H Blumin; J E Donelson; L V Kirchhoff
Journal:  Infect Immun       Date:  1989-07       Impact factor: 3.441

10.  Studies on trypanosomatid actin. I. Immunochemical and biochemical identification.

Authors:  R A Mortara
Journal:  J Protozool       Date:  1989 Jan-Feb
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  13 in total

1.  Physical mapping of a 670-kb region of chromosomes XVI and XVII from the human protozoan parasite Trypanosoma cruzi encompassing the genes for two immunodominant antigens.

Authors:  M R Santos; H Lorenzi; P Porcile; M S Carmo; A Schijman; A Brandão; J E Araya; H B Gomes; M A Chiurillo; J L Ramirez; W M Degrave; M J Levin; J F da Silveira
Journal:  Genome Res       Date:  1999-12       Impact factor: 9.043

2.  The putative telomerase reverse transcriptase component of Leishmania amazonensis: gene cloning and characterization.

Authors:  Miriam A Giardini; Cristina B B Lira; Fábio F Conte; Luciana R Camillo; Jair L de Siqueira Neto; Carlos H I Ramos; Maria Isabel N Cano
Journal:  Parasitol Res       Date:  2006-01-14       Impact factor: 2.289

3.  Comparison of recombinant Trypanosoma cruzi peptide mixtures versus multiepitope chimeric proteins as sensitizing antigens for immunodiagnosis.

Authors:  Cecilia Camussone; Verónica Gonzalez; María S Belluzo; Nazarena Pujato; María E Ribone; Claudia M Lagier; Iván S Marcipar
Journal:  Clin Vaccine Immunol       Date:  2009-04-01

4.  Serological diagnosis of Chagas' disease: a potential confirmatory assay using preserved protein antigens of Trypanosoma cruzi.

Authors:  R P Mendes; S Hoshino-Shimizu; A M Moura da Silva; I Mota; R A Heredia; A O Luquetti; P G Leser
Journal:  J Clin Microbiol       Date:  1997-07       Impact factor: 5.948

5.  Evaluation of recombinant antigens for serodiagnosis of Chagas' disease in South and Central America.

Authors:  E S Umezawa; S F Bastos; M E Camargo; L M Yamauchi; M R Santos; A Gonzalez; B Zingales; M J Levin; O Sousa; R Rangel-Aldao; J F da Silveira
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

6.  Serodiagnosis of Chagas' disease by enzyme-linked immunosorbent assay using two synthetic peptides as antigens.

Authors:  J M Peralta; M G Teixeira; W G Shreffler; J B Pereira; J M Burns; P R Sleath; S G Reed
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

Review 7.  Chagas Disease Diagnostic Applications: Present Knowledge and Future Steps.

Authors:  V Balouz; F Agüero; C A Buscaglia
Journal:  Adv Parasitol       Date:  2016-11-14       Impact factor: 3.870

8.  Characterization of a cDNA clone encoding the carboxy-terminal domain of a 90-kilodalton surface antigen of Trypanosoma cruzi metacyclic trypomastigotes.

Authors:  F R Franco; G S Paranhos-Bacallà; L M Yamauchi; N Yoshida; J F da Silveira
Journal:  Infect Immun       Date:  1993-10       Impact factor: 3.441

9.  The Leishmania amazonensis TRF (TTAGGG repeat-binding factor) homologue binds and co-localizes with telomeres.

Authors:  Marcelo S da Silva; Arina M Perez; Rita de Cássia V da Silveira; Camila E de Moraes; Jair L Siqueira-Neto; Lucio de H Freitas; Maria Isabel N Cano
Journal:  BMC Microbiol       Date:  2010-05-07       Impact factor: 3.605

Review 10.  Chagas' disease.

Authors:  H B Tanowitz; L V Kirchhoff; D Simon; S A Morris; L M Weiss; M Wittner
Journal:  Clin Microbiol Rev       Date:  1992-10       Impact factor: 26.132
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