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Literature DB >> 6181502

Expression of a chemically synthesized human alpha 1 interferon gene.

E De Maeyer, D Skup, K S Prasad, J De Maeyer-Guignard, B Williams, P Meacock, G Sharpe, D Pioli, J Hennam, W Schuch, K Atherton.

Abstract

Cells of Escherichia coli containing a chemically synthesized human alpha 1 interferon (IFN-alpha 1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-alpha 1 [antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2'-5')oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-alpha 1 gene) was transplanted into the obligate methylotroph Methylophilus methylotrophus.

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Year: 1982 PMID： 6181502 PMCID： PMC346649 DOI： 10.1073/pnas.79.14.4256

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Eschericha coli.

Authors: P M Bennett; M H Richmond
Journal: J Bacteriol Date: 1976-04 Impact factor: 3.490

2. A family of structural genes for human lymphoblastoid (leukocyte-type) interferon.

Authors: G Allen; K H Fantes
Journal: Nature Date: 1980-10-02 Impact factor: 49.962

3. The nucleotide sequence of a cloned human leukocyte interferon cDNA.

Authors: N Mantei; M Schwarzstein; M Streuli; S Panem; S Nagata; C Weissmann
Journal: Gene Date: 1980-06 Impact factor: 3.688

4. The structure of eight distinct cloned human leukocyte interferon cDNAs.

Authors: D V Goeddel; D W Leung; T J Dull; M Gross; R M Lawn; R McCandliss; P H Seeburg; A Ullrich; E Yelverton; P W Gray
Journal: Nature Date: 1981-03-05 Impact factor: 49.962

5. Bacterial synthesis of a novel human leukocyte interferon.

Authors: E Yelverton; D Leung; P Weck; P W Gray; D V Goeddel
Journal: Nucleic Acids Res Date: 1981-02-11 Impact factor: 16.971

6. Cloned synthetic lac operator DNA is biologically active.

Authors: K J Marians; R Wu; J Stawinski; T Hozumi; S A Narang
Journal: Nature Date: 1976-10-28 Impact factor: 49.962

7. Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus.

Authors: J D Windass; M J Worsey; E M Pioli; D Pioli; P T Barth; K T Atherton; E C Dart; D Byrom; K Powell; P J Senior
Journal: Nature Date: 1980-10-02 Impact factor: 49.962

8. Cloning of bovine growth hormone gene and its expression in bacteria.

Authors: E Keshet; A Rosner; Y Bernstein; M Gorecki; H Aviv
Journal: Nucleic Acids Res Date: 1981-01-10 Impact factor: 16.971

9. Target cell specificity of two species of human interferon-alpha produced in Escherichia coli and of hybrid molecules derived from them.

Authors: M Streuli; A Hall; W Boll; W E Stewart; S Nagata; C Weissmann
Journal: Proc Natl Acad Sci U S A Date: 1981-05 Impact factor: 11.205

10. Comparisons of several biological and physicochemical properties of human leukocyte interferons produced my human leukocytes and by E. coli.

Authors: W E Stewart; F H Sarkar; H Taira; A Hall; S Nagata; C Weissmann
Journal: Gene Date: 1980-11 Impact factor: 3.688

11 in total

Review 1. The purification of eukaryotic polypeptides synthesized in Escherichia coli.

Authors: F A Marston
Journal: Biochem J Date: 1986-11-15 Impact factor: 3.857

2. High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector.

Authors: E Jay; J Rommens; L Pomeroy-Cloney; D MacKnight; C Lutze-Wallace; P Wishart; D Harrison; W Y Lui; V Asundi; M Dawood
Journal: Proc Natl Acad Sci U S A Date: 1984-04 Impact factor: 11.205

3. Selective production of interferon-alpha subtypes by cultured peripheral blood mononuclear cells and lymphoblastoid cell lines.

Authors: A L Greenway; M L Overall; N Sattayasai; M J Rowley; P J Hertzog; G L McMullen; B F Cheetham; S Marzuki
Journal: Immunology Date: 1992-01 Impact factor: 7.397

4. Interferon-induced 2-5A synthetase activity in human peripheral blood mononuclear cells after immunization with influenza virus and rubella virus vaccines.

Authors: L J Penn; B R Williams
Journal: J Virol Date: 1984-03 Impact factor: 5.103

5. Role of the SulB (FtsZ) protein in division inhibition during the SOS response in Escherichia coli: FtsZ stabilizes the inhibitor SulA in maxicells.

Authors: C Jones; I B Holland
Journal: Proc Natl Acad Sci U S A Date: 1985-09 Impact factor: 11.205