Literature DB >> 6326118

High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector.

E Jay, J Rommens, L Pomeroy-Cloney, D MacKnight, C Lutze-Wallace, P Wishart, D Harrison, W Y Lui, V Asundi, M Dawood.   

Abstract

A chemically synthesized gene for human interferon-gamma has been cloned into a prokaryotic expression vector under the regulation of a synthetic constitutive transcriptional-translational control unit that contains a strong bacteriophage T5 early promoter and a strong ribosome-binding site. Cells harboring the recombinant plasmid express high levels (4 X 10(9) units per liter of culture) of antiviral activity specific for interferon-gamma. Analysis of total cell lysates on NaDodSO4/polyacrylamide gels revealed a 17,200-dalton protein, expected for the nonglycosylated form of human interferon-gamma, that constitutes greater than 15% of total cell protein.

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Year:  1984        PMID： 6326118      PMCID： PMC345044          DOI： 10.1073/pnas.81.8.2290

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  21 in total

1.  Interaction of E. coli RNA polymerase with promotors of coliphage T5: the rates of complex formation and decay and their correlation with in vitro and in vivo transcriptional activity.

Authors:  A von Gabain; H Bujard
Journal:  Mol Gen Genet       Date:  1977-12-09

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  5'-32P labeling of RNA and DNA restriction fragments.

Authors:  G Chaconas; J H van de Sande
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

4.  Synthesis of human fibroblast interferon by E. coli.

Authors:  D V Goeddel; H M Shepard; E Yelverton; D Leung; R Crea; A Sloma; S Pestka
Journal:  Nucleic Acids Res       Date:  1980-09-25       Impact factor: 16.971

5.  Bacterial synthesis of a novel human leukocyte interferon.

Authors:  E Yelverton; D Leung; P Weck; P W Gray; D V Goeddel
Journal:  Nucleic Acids Res       Date:  1981-02-11       Impact factor: 16.971

6.  Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit beta-globin.

Authors:  L Guarente; G Lauer; T M Roberts; M Ptashne
Journal:  Cell       Date:  1980-06       Impact factor: 41.582

7.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

8.  Human leukocyte interferon produced by E. coli is biologically active.

Authors:  D V Goeddel; E Yelverton; A Ullrich; H L Heyneker; G Miozzari; W Holmes; P H Seeburg; T Dull; L May; N Stebbing; R Crea; S Maeda; R McCandliss; A Sloma; J M Tabor; M Gross; P C Familletti; S Pestka
Journal:  Nature       Date:  1980-10-02       Impact factor: 49.962

9.  Expression of the human fibroblast interferon gene in Escherichia coli.

Authors:  T Taniguchi; L Guarente; T M Roberts; D Kimelman; J Douhan; M Ptashne
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

10.  Purified lambda regulatory protein cII positively activates promoters for lysogenic development.

Authors:  H Simatake; M Rosenberg
Journal:  Nature       Date:  1981-07-09       Impact factor: 49.962
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  8 in total

1.  A plasmid cloning vector with precisely regulatable copy number in Escherichia coli.

Authors:  A Herman-Antosiewicz; M Obuchowski; G Wegrzyn
Journal:  Mol Biotechnol       Date:  2001-03       Impact factor: 2.695

2.  Construction of a Co1E1 plasmid bearing inducible high-copy-number phenotype.

Authors:  D Bachvarov; E Jay; I Ivanov
Journal:  Folia Microbiol (Praha)       Date:  1990       Impact factor: 2.099

3.  Immunobiological study of interferon-gamma-producing cells after staphylococcal enterotoxin B stimulation.

Authors:  C L Lee; S H Lee; F T Jay; K R Rozee
Journal:  Immunology       Date:  1990-05       Impact factor: 7.397

4.  Simultaneous synthesis of human-, mouse- and chimeric epidermal growth factor genes via 'hybrid gene synthesis' approach.

Authors:  W L Sung; D M Zahab; F L Yao; R Wu; S A Narang
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

5.  Expression of the complete human T-cell leukemia virus type I pX coding sequence as a functional protein in Escherichia coli.

Authors:  C Z Giam; M Nerenberg; G Khoury; G Jay
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

6.  Expression of the Escherichia coli trpE gene in E. coli K12 bacteria: maximum level, rate and time of initiation of anthranilate synthetase production.

Authors:  H G Hessing; C van Rotterdam; P H Pouwels
Journal:  Mol Gen Genet       Date:  1987-12

7.  Human interferon-gamma has three domains associated with its antiviral function: a neutralizing epitope typing scheme for human interferon-gamma.

Authors:  A Y Kwok; X Zu; C Yang; M J Alfa; F T Jay
Journal:  Immunology       Date:  1993-05       Impact factor: 7.397

8.  The influence of mRNA primary and secondary structure on human IFN-gamma gene expression in E. coli.

Authors:  L H Tessier; P Sondermeyer; T Faure; D Dreyer; A Benavente; D Villeval; M Courtney; J P Lecocq
Journal:  Nucleic Acids Res       Date:  1984-10-25       Impact factor: 16.971
  8 in total

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