Literature DB >> 792711

Cloned synthetic lac operator DNA is biologically active.

K J Marians, R Wu, J Stawinski, T Hozumi, S A Narang.   

Abstract

A chemically synthesised duplex DNA fragment containing the sequence of the lac operator was cloned in E. coli using the vehicle pMB9. Clones containing lac-pMB9 hybrid DNA produced beta-galactosidase constitutively and the hybrid DNA bound the lac repressor specifically.

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Year:  1976        PMID： 792711     DOI： 10.1038/263744a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  20 in total

1.  A rapid and convenient synthesis of poly-thymidylic acid by the modified triester approach.

Authors:  A K Sood; S A Narang
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

2.  Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor.

Authors:  C P Bahl; R Wu; J Stawinsky; S A Narang
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

3.  Medical Research Council committee draws up guidelines for research into recombinant DNA.

Authors:  M Mitchell; J G Kaplan
Journal:  Can Med Assoc J       Date:  1977-04-09       Impact factor: 8.262

Review 4.  Molecular cloning of DNA. An introduction into techniques and problems.

Authors:  H P Vosberg
Journal:  Hum Genet       Date:  1977-12-29       Impact factor: 4.132

5.  Regulation of the F-factor pif operon: pifO, a site required in cis for autoregulation, titrates the pifC product in trans.

Authors:  J F Miller; M H Malamy
Journal:  J Bacteriol       Date:  1984-10       Impact factor: 3.490

6.  Construction and expression of hybrid plasmids containing the structural gene of the Escherichia coli K-12 3-deoxy-2-oxo-D-gluconate transport system.

Authors:  M A Mandrand-Berthelot; P Ritzenthaler; M Mata-Gilsinger
Journal:  J Bacteriol       Date:  1984-11       Impact factor: 3.490

7.  Construction of hybrid plasmids containing the Escherichia coli uxaB gene: analysis of its regulation and direction of transcription.

Authors:  C Blanco; M Mata-Gilsinger; P Ritzenthaler
Journal:  J Bacteriol       Date:  1983-02       Impact factor: 3.490

8.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

9.  Excision of DNA segments introduced into cloning vectors by the poly(dA-dT) joining method.

Authors:  S P Goff; P Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

10.  Avian myeloblastosis virus transforming gene is related to unique chicken DNA regions separated by at least one intervening sequence.

Authors:  B Perbal; M A Baluda
Journal:  J Virol       Date:  1982-01       Impact factor: 5.103
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