Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Effect of DNA length on the nucleosome low salt transition.

Literature DB >> 6777754

Effect of DNA length on the nucleosome low salt transition.

A E Dieterich, H Eshaghpour, D M Crothers, C R Cantor.

Abstract

The effect of DNA length on the low salt unfolding transition of nucleosomes has been studied by the use of fluorescently labeled histones. Nucleosomes were formed by the reconstitution of bulk DNA fragments averaging 173 and 250 base pairs in length. These nucleosomes exhibited a conformational change in a transition centered at about 7 mM ionic strength, very different from that observed for the standard 145 bp nucleosomes (1-3mM). In addition, the conformational change of the 173 and 250 bp nucleosomes involves twice as many ions as that of the 145 bp nucleosomes.

Entities: Chemical

Mesh：

Substances：

Year: 1980 PMID： 6777754 PMCID： PMC324095 DOI： 10.1093/nar/8.11.2475

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

25 in total

Effect of DNA length on the nucleosome low salt transition.

1. Chromatin nu bodies: isolation, subfractionation and physical characterization.

2. Ion effects on ligand-nucleic acid interactions.

3. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.

4. Chromosomal subunits in active genes have an altered conformation.

5. Structure of nucleosome core particles of chromatin.

6. Singlet energy transfer studies of the arrangement of proteins in the 30 S Escherichia coli ribosome.

7. A new method for sequencing DNA.

8. An electrophoretic comparison of vertebrate histones.

9. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

10. A modified procedure for fractionating histones.

1. Reversibility of the low-salt transition of chromatin core particles.

2. Stability of the primary organization of nucleosome core particles upon some conformational transitions.

3. The roles of H1, the histone core and DNA length in the unfolding of nucleosomes at low ionic strength.