Literature DB >> 228251

Specific chemical labeling of DNA fragments.

H Eshaghpour, D Söll, D M Crothers.   

Abstract

We describe a simple method for specific chemical labeling of DNA fragments at their 3'-termini. The procedure includes enzymatic addition of 4-thiouridine, followed by reaction in mild non-denaturing conditions with the highly reactive alpha-haloacetamido derivatives of several chemical labels. The attached reporter molecule can be removed by extended treatment with beta-mercaptoethanol. Among the potential applications of this labeling method is the study of specific protein-DNA interactions in solution.

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Year:  1979        PMID： 228251      PMCID： PMC342322          DOI： 10.1093/nar/7.6.1485

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  Properies of tRNAPhe from yeast carrying a spin label on the 3'-terminal. Interaction with yeast phenylalanyl-tRNA Synthetase and elongation factor Tu from Escherichia coli.

Authors:  M Sprinzl; G E Siboska; J A Pedersen
Journal:  Nucleic Acids Res       Date:  1978-03       Impact factor: 16.971

2.  Preparative separation of the complementary strands of DNA restriction fragments by alkaline RPC-5 chromatography.

Authors:  H Eshaghpour; D M Crothers
Journal:  Nucleic Acids Res       Date:  1978-01       Impact factor: 16.971

3.  Analogs of methionyl-tRNA synthetase substrates containing photolabile groups.

Authors:  R Wetzel; D Söll
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

4.  4-Thiouridine-specific spin-labeling of E. coli transfer RNA.

Authors:  H Hara; T Horiuchi; M Saneyoshi; S Nishimura
Journal:  Biochem Biophys Res Commun       Date:  1970-01-23       Impact factor: 3.575

5.  DNAase I, DNAase II and staphylococcal nuclease cut at different, yet symmetrically located, sites in the nucleosome core.

Authors:  B Sollner-Webb; W Melchior; G Felsenfeld
Journal:  Cell       Date:  1978-07       Impact factor: 41.582

6.  Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin.

Authors:  L Klevan; D M Crothers
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971

7.  [Addition of the fluorescent label to the 3'-OH end of DNA and the 3'-OH end of nascent RNA].

Authors:  T A Rozovskaia; P Sh Bibilashvili; N B Tarusova; G V Gurskiĭ; S A Strel'tsov
Journal:  Mol Biol (Mosk)       Date:  1977 May-Jun

8.  Studies of transfer RNA tertiary structure of singlet-singlet energy transfer.

Authors:  C H Yang; D Söll
Journal:  Proc Natl Acad Sci U S A       Date:  1974-07       Impact factor: 11.205

9.  The effect of specific structural modification on the biological activity of E. coli arginine tRNA.

Authors:  T A Kruse; B F Clark
Journal:  Nucleic Acids Res       Date:  1978-03       Impact factor: 16.971

10.  Photo-affinity labeling of tRNA binding sites in macromolecules. I. Linking of the phenacyl-p-azide of 4-thiouridine in (Escherichia coli) valyl-tRNA to 16S RNA at the ribosomal P site.

Authors:  I Schwartz; J Ofengand
Journal:  Proc Natl Acad Sci U S A       Date:  1974-10       Impact factor: 11.205
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  4 in total

1.  Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.

Authors:  R Cosstick; L W McLaughlin; F Eckstein
Journal:  Nucleic Acids Res       Date:  1984-02-24       Impact factor: 16.971

2.  Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probes.

Authors:  B A Connolly; P Rider
Journal:  Nucleic Acids Res       Date:  1985-06-25       Impact factor: 16.971

3.  Selective fluorescence of eosinophilic structures in grasshopper and mammalian testis stained with haematoxylin-eosin.

Authors:  J Espada; P Valverde; J C Stockert
Journal:  Histochemistry       Date:  1993-05

4.  Synthesis and use of labelled nucleoside phosphoramidite building blocks bearing a reporter group: biotinyl, dinitrophenyl, pyrenyl and dansyl.

Authors:  A Roget; H Bazin; R Teoule
Journal:  Nucleic Acids Res       Date:  1989-10-11       Impact factor: 16.971
  4 in total

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