Literature DB >> 5084801

Kinetics of the reversible reaction of papain with 5,5'-dithiobis-(2-nitrobenzoate) dianion: evidence for nucleophilic reactivity in the un-ionized thiol group of cysteine-25 and for general acid catalysis by histidine-159 of the reaction of the 5-mercapto-2-nitrobenzoate dianion with the papain-5-mercapto-2-nitrobenzoate mixed disulphide.

G Little, K Brocklehurst.   

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Year:  1972        PMID： 5084801      PMCID： PMC1173787          DOI： 10.1042/bj1280475

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


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  4 in total

1.  A novel reactivity of papain and a convenient active site titration in the presence of other thiols.

Authors:  K Brocklehurst; G Little
Journal:  FEBS Lett       Date:  1970-07-29       Impact factor: 4.124

2.  The effect of pH on the affinities of enzymes for substrates and inhibitors.

Authors:  M DIXON
Journal:  Biochem J       Date:  1953-08       Impact factor: 3.857

3.  Reaction of protein disulfide groups with Ellman's reagent: a case study of the number of sulfhydryl and disulfide groups in Aspergillus oryzae -amylase, papain, and lysozyme.

Authors:  J F Robyt; R J Ackerman; C G Chittenden
Journal:  Arch Biochem Biophys       Date:  1971-11       Impact factor: 4.013

4.  Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system.

Authors:  K Brocklehurst; G Little
Journal:  Biochem J       Date:  1972-06       Impact factor: 3.857
  4 in total
  7 in total

1.  Alkylation of glyceraldehyde-3-phosphate dehydrogenase with haloacetylphosphonates. An unusual pH-dependence.

Authors:  Y K Li; J Boggaram; L D Byers
Journal:  Biochem J       Date:  1991-05-01       Impact factor: 3.857

2.  Covalent chromatography. Preparation of fully active papain from dried papaya latex.

Authors:  K Brocklehurst; J Carlsson; M P Kierstan; E M Crook
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857

3.  The effect of some amine oxides and disulphide compounds on the activity of acetylcholinesterase.

Authors:  R M Dawson
Journal:  Biochem J       Date:  1975-07       Impact factor: 3.857

4.  Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophils.

Authors:  J Carlsson; M P Kierstan; K Brocklehurst
Journal:  Biochem J       Date:  1974-04       Impact factor: 3.857

5.  Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.

Authors:  K Brocklehurst; G Little
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857

6.  Substrate-derived two-protonic-state electrophiles as sensitive kinetic specificity probes for cysteine proteinases. Activation of 2-pyridyl disulphides by hydrogen-bonding.

Authors:  K Brocklehurst; D Kowlessur; M O'Driscoll; G Patel; S Quenby; E Salih; W Templeton; E W Thomas; F Willenbrock
Journal:  Biochem J       Date:  1987-05-15       Impact factor: 3.857

7.  Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system.

Authors:  K Brocklehurst; G Little
Journal:  Biochem J       Date:  1972-06       Impact factor: 3.857
  7 in total

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