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Literature DB >> 4910855

Peptide chain termination. VII. The ribosomal and release factor requirements for peptide release.

Abstract

Release factors participate in release of fMet from fMet-tRNA . AUG . ribosome intermediates upon binding to ribosomes. This release requires R factor and occurs in the absence of terminator codon in reactions containing 20 per cent ethanol. Release occurs only when both 30S and 50S ribosomal subunits are present and when fMet-tRNA is located in the ribosomal P site. Release factor-dependent deacylation of fMet-tRNA is inhibited by sparsomycin, amicetin, lincocin, and chloramphenicol, antibiotics which have little effect on binding of R factor to ribosomes. The possible role of peptidyl transferase in the release reaction is discussed.

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Year: 1970 PMID： 4910855 PMCID： PMC282963 DOI： 10.1073/pnas.65.3.702

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

18 in total

Peptide chain termination. VII. The ribosomal and release factor requirements for peptide release.

1. Role of guanosine 5'-triphosphate in the initiation of Peptide synthesis, I. Synthesis of formylmethionyl-puromycin.

2. Separation of three microbial amino acid polymerization factors.

3. Antibiotic inhibitors of the bacterial ribosome.

4. The possible involvement of peptidyl transferase in the termination step of protein biosynthesis.

5. On the specificity and stability of an enzyme that hydrolyzes N-substituted aminoacyl-transfer RNA's.

6. Transesterification by peptidyl transferase.

7. Regulatory mechanisms and protein synthesis. X. Codon recognition on 30 S ribosomes.

8. Ribosome-catalysed peptidyl transfer: effects of some inhibitors of protein synthesis.

9. Sequential translation of trinucleotide codons for the initiation and termination of protein synthesis.

10. RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.

1. Recognition of the amber UAG stop codon by release factor RF1.

2. Use of single-stranded DNA oligonucleotides in programming ribosomes for translation.

Review 3. Structural aspects of translation termination on the ribosome.

4. The requirement for ribosomal proteins L7 and L12 in peptide-chain termination.

Review 5. Translation of the genome of a ribonucleic acid bacteriophage.

Review 6. The mechanism of peptide chain termination.

7. DNA-directed synthesis in vitro of beta-galactosidase: requirement for a ribosome release factor.

8. Peptide chain termination with mammalian release factor.

9. Hydrolysis of fMet-tRNA by peptidyl transferase.

10. Cloning of the Escherichia coli release factor 2 gene.