Literature DB >> 4943558

Hydrolysis of fMet-tRNA by peptidyl transferase.

C T Caskey, A L Beaudet, E M Scolnick, M Rosman.   

Abstract

Escherichia coli and rabbit reticulocyte (f[(3)H]Met-tRNA.AUG.ribosome) intermediates undergo hydrolysis, with release of f[(3)H]methionine, upon addition of tRNA or CpCpA in the presence of acetone. This ribosomal catalyzed reaction has similar requirements, pH optimum, and antibiotic sensitivity to those of peptidyl transferase. Two antibiotics, lincomycin with E. coli ribosomes and anisomycin with reticulocyte ribosomes, inhibit peptide-bond formation and transesterification activities of peptidyl transferase, but stimulate hydrolysis of f[(3)H]Met-tRNA. Earlier studies have suggested peptidyl transferase activity is essential for R factor-dependent hydrolysis of f((3)H)Met-tRNA. These studies indicate that peptidyl transferase has the capacity for f((3)H)Met-tRNA hydrolysis and, therefore, may be responsible for peptidyl-tRNA cleavage during peptide chain termination.

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Year:  1971        PMID： 4943558      PMCID： PMC389613          DOI： 10.1073/pnas.68.12.3163

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  The possible involvement of peptidyl transferase in the termination step of protein biosynthesis.

Authors:  Z Vogel; A Zamir; D Elson
Journal:  Biochemistry       Date:  1969-12       Impact factor: 3.162

2.  The peptidyl transferase activity of ribosomes.

Authors:  R E Monro; T Staehelin; M L Celma; D Vazquez
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1969

3.  Ribosome-catalyzed peptidyl transfer. Effects of cations and pH value.

Authors:  B E Maden; R E Monro
Journal:  Eur J Biochem       Date:  1968-11

4.  Peptide chain termination. 3. Stimulation of in vitro termination.

Authors:  G Milman; J Goldstein; E Scolnick; T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1969-05       Impact factor: 11.205

5.  Substrate specificity of ribosomal peptidyl transferase: 2'(3')-O-aminoacyl nucleosides as acceptors of the peptide chain on the amino acid site.

Authors:  I Rychlík; J Cerná; S Chládek; J Zemlicka; Z Haladová
Journal:  J Mol Biol       Date:  1969-07-14       Impact factor: 5.469

6.  Transesterification by peptidyl transferase.

Authors:  E Scolnick; G Milman; M Rosman; T Caskey
Journal:  Nature       Date:  1970-01-10       Impact factor: 49.962

7.  Initiation of protein synthesis II. A convenient assay for the ribosome-dependent synthesis of N-formyl-C14-methionylpuromycin.

Authors:  P Leder; H Bursztyn
Journal:  Biochem Biophys Res Commun       Date:  1966-10-20       Impact factor: 3.575

8.  Action of sparsomycin on ribosome-catalysed peptidyl transfer.

Authors:  R E Monro; M L Celma; D Vazquez
Journal:  Nature       Date:  1969-04-26       Impact factor: 49.962

9.  Ribosome-catalysed peptidyl transfer: effects of some inhibitors of protein synthesis.

Authors:  R E Monro; D Vazquez
Journal:  J Mol Biol       Date:  1967-08-28       Impact factor: 5.469

10.  Inhibition by siomycin and thiostrepton of both aminoacyl-tRNA and factor G binding to ribosomes.

Authors:  J Modolell; B Cabrer; A Parmeggiani; D Vazquez
Journal:  Proc Natl Acad Sci U S A       Date:  1971-08       Impact factor: 11.205
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  35 in total

1.  Suppression of nonsense mutations induced by expression of an RNA complementary to a conserved segment of 23S rRNA.

Authors:  N S Chernyaeva; E J Murgola; A S Mankin
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

2.  Structure of the 70S ribosome bound to release factor 2 and a substrate analog provides insights into catalysis of peptide release.

Authors:  Hong Jin; Ann C Kelley; David Loakes; V Ramakrishnan
Journal:  Proc Natl Acad Sci U S A       Date:  2010-04-26       Impact factor: 11.205

3.  Bioinformatic, structural, and functional analyses support release factor-like MTRF1 as a protein able to decode nonstandard stop codons beginning with adenine in vertebrate mitochondria.

Authors:  David J Young; Christina D Edgar; Jennifer Murphy; Johannes Fredebohm; Elizabeth S Poole; Warren P Tate
Journal:  RNA       Date:  2010-04-26       Impact factor: 4.942

Review 4.  The roles of RNA in the synthesis of protein.

Authors:  Peter B Moore; Thomas A Steitz
Journal:  Cold Spring Harb Perspect Biol       Date:  2011-11-01       Impact factor: 10.005

5.  The codon specificity of eubacterial release factors is determined by the sequence and size of the recognition loop.

Authors:  David J Young; Christina D Edgar; Elizabeth S Poole; Warren P Tate
Journal:  RNA       Date:  2010-06-28       Impact factor: 4.942

6.  Recognition of the amber UAG stop codon by release factor RF1.

Authors:  Andrei Korostelev; Jianyu Zhu; Haruichi Asahara; Harry F Noller
Journal:  EMBO J       Date:  2010-06-29       Impact factor: 11.598

7.  Evolution of protein synthesis from an RNA world.

Authors:  Harry F Noller
Journal:  Cold Spring Harb Perspect Biol       Date:  2012-04-01       Impact factor: 10.005

8.  The interaction between C75 of tRNA and the A loop of the ribosome stimulates peptidyl transferase activity.

Authors:  Julie L Brunelle; Elaine M Youngman; Divya Sharma; Rachel Green
Journal:  RNA       Date:  2006-01       Impact factor: 4.942

9.  Two distinct components of release factor function uncovered by nucleophile partitioning analysis.

Authors:  Jeffrey J Shaw; Rachel Green
Journal:  Mol Cell       Date:  2007-11-09       Impact factor: 17.970

10.  Peptidyl-CCA deacylation on the ribosome promoted by induced fit and the O3'-hydroxyl group of A76 of the unacylated A-site tRNA.

Authors:  Miljan Simonović; Thomas A Steitz
Journal:  RNA       Date:  2008-09-25       Impact factor: 4.942
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