Literature DB >> 4844271

Comparison of rapid methods for analysis of bacterial fatty acids.

C W Moss, M A Lambert, W H Merwin.   

Abstract

When rapid gas-liquid chromatography methods for determination of bacterial fatty acids were compared, results showed that saponification was required for total fatty acid analysis. Transesterification with boron-trihalide reagents (BF(3)-CH(3)OH, BCl(3)-CH(3)OH) caused extensive degradation of cyclopropane acids and was less effective than saponification in releasing cellular hydroxy fatty acids. Digestion of cells with tetramethylammonium hydroxide was unsatisfactory because of extraneous gas-liquid chromatography peaks and because of lower recovery of branched-chain and hydroxy fatty acids. A simple, rapid saponification procedure which can be used for total cellular fatty acid analysis of freshly grown cells is described.

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Year:  1974        PMID： 4844271      PMCID： PMC186596          DOI： 10.1128/am.28.1.80-85.1974

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  13 in total

1.  Characterization of five clostridial pathogens by gas-liquid chromatography.

Authors:  R D Ellender; R J Hidalgo; L C Grumbles
Journal:  Am J Vet Res       Date:  1970-10       Impact factor: 1.156

2.  Fatty acid fingerprints of Streptococcus mutans grown in a chemostat.

Authors:  D B Drucker; C J Griffit; T H Melville
Journal:  Microbios       Date:  1973

3.  Cellular fatty acid composition of selected Pseudomonas species.

Authors:  C W Moss; S B Samuels; R E Weaver
Journal:  Appl Microbiol       Date:  1972-10

4.  Differentiation between Mycobacterium kansasii and Mycobacterium marinum by gas-liquid chromatographic analysis of cellular fatty acids.

Authors:  C O Thoen; A G Karlson; R D Ellefson
Journal:  Appl Microbiol       Date:  1972-12

5.  Mass spectral discrimination between monoenoic and cyclopropanoid, and between normal, iso, and anteiso fatty acid methyl esters.

Authors:  I M Campbell; J Naworal
Journal:  J Lipid Res       Date:  1969-09       Impact factor: 5.922

6.  Occurrence of branched-cahin hydroxy fatty acids in Pseudomonas maltophilia.

Authors:  C W Moss; S B Samuels; J Liddle; R M McKinney
Journal:  J Bacteriol       Date:  1973-06       Impact factor: 3.490

7.  Production of an artifact during methanolysis of lipids by boron trifluoride-methanol.

Authors:  W K Fulk; M S Shorb
Journal:  J Lipid Res       Date:  1970-05       Impact factor: 5.922

8.  Occurrence of 3-hydroxytridecanoic and 3-hydroxypentadecanoic acids in the lipopolysaccharides of Veillonella.

Authors:  D G Bishop; M J Hewett; K W Knox
Journal:  Biochim Biophys Acta       Date:  1971-03-16

9.  Artifacts produced by boron trifluoride methanolysis of a synthetic lecithin containing cyclopropane fatty acids (1-2-dihydrosterculoyl-3-sn-phosphatidylcholine).

Authors:  E A Dawidowicz; T E Thompson
Journal:  J Lipid Res       Date:  1971-09       Impact factor: 5.922

10.  On methylation of unsaturated acids using boron trihalide-methanol reagents.

Authors:  W E Klopfenstein
Journal:  J Lipid Res       Date:  1971-11       Impact factor: 5.922
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  35 in total

1.  Novel chemoautotrophic endosymbiosis between a member of the Epsilonproteobacteria and the hydrothermal-vent gastropod Alviniconcha aff. hessleri (Gastropoda: Provannidae) from the Indian Ocean.

Authors:  Yohey Suzuki; Takenori Sasaki; Masae Suzuki; Yuichi Nogi; Tetsuya Miwa; Ken Takai; Kenneth H Nealson; Koki Horikoshi
Journal:  Appl Environ Microbiol       Date:  2005-09       Impact factor: 4.792

2.  Formation of trans fatty acids is not involved in growth-linked membrane adaptation of Pseudomonas putida.

Authors:  Claus Härtig; Norbert Loffhagen; Hauke Harms
Journal:  Appl Environ Microbiol       Date:  2005-04       Impact factor: 4.792

3.  Biochemical function and ecological significance of novel bacterial lipids in deep-sea procaryotes.

Authors:  E F Delong; A A Yayanos
Journal:  Appl Environ Microbiol       Date:  1986-04       Impact factor: 4.792

4.  Stability of lactic Acid bacteria to freezing as related to their Fatty Acid composition.

Authors:  I Goldberg; L Eschar
Journal:  Appl Environ Microbiol       Date:  1977-03       Impact factor: 4.792

5.  Combined Determination of Poly-beta-Hydroxyalkanoic and Cellular Fatty Acids in Starved Marine Bacteria and Sewage Sludge by Gas Chromatography with Flame Ionization or Mass Spectrometry Detection.

Authors:  G Odham; A Tunlid; G Westerdahl; P Mårdén
Journal:  Appl Environ Microbiol       Date:  1986-10       Impact factor: 4.792

6.  Evaluation of fatty acid profile, color characteristics, oxidative quality and stability of common Kilka (Clupeonella cultriventris caspia) oil obtained by various extraction techniques.

Authors:  Ruhollah Sayyad; Mahdi Ghomi
Journal:  J Food Sci Technol       Date:  2017-03-13       Impact factor: 2.701

7.  Rapid diagnosis of lymphocytic meningitis by frequency-pulsed electron capture gas-liquid chromatography: differentiation of tuberculous, cryptococcal, and viral meningitis.

Authors:  R B Craven; J B Brooks; D C Edman; J D Converse; J Greenlee; D Schlossberg; T Furlow; J M Gwaltney; W F Miner
Journal:  J Clin Microbiol       Date:  1977-07       Impact factor: 5.948

8.  Fatty acid composition of Streptococcus milleri.

Authors:  M Labbé; P Van der Auwera; Y Glupczynski; F Crockaert; E Yourassowsky
Journal:  Eur J Clin Microbiol       Date:  1985-08       Impact factor: 3.267

9.  Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria.

Authors:  G O Guerrant; M A Lambert; C W Moss
Journal:  J Clin Microbiol       Date:  1981-05       Impact factor: 5.948

10.  Rapid method for the detection and identification of mycolic acids in aerobic actinomycetes and related bacteria.

Authors:  S T Hecht; W A Causey
Journal:  J Clin Microbiol       Date:  1976-09       Impact factor: 5.948
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