Literature DB >> 7240402

Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria.

G O Guerrant, M A Lambert, C W Moss.   

Abstract

Mycolic acids were detected in both reference strains and clinical isolates of mycobacteria using gas chromatography of fatty acid methyl esters prepared by acid methanolysis. The methyl esters were extracted with hexane, concentrated, and analyzed with a gas chromatograph by using two different injector temperatures. When the samples were analyzed at high injector temperatures of 300 to 350 degrees C, characteristic thermal cleavage products from mycolic acids, C22:0, C24:0, or C26:0 fatty acid methyl esters, were detected. When analyzed at injector temperatures of 235 degrees C or lower, the mycolic acids were heat stable and the characteristic methyl ester cleavage products were not observed.

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Year:  1981        PMID： 7240402      PMCID： PMC273914          DOI： 10.1128/jcm.13.5.899-907.1981

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

Review 1.  Mycobacterial lipids: selected topics.

Authors:  M B Goren
Journal:  Bacteriol Rev       Date:  1972-03

2.  New approach to derivatization and gas-chromatographic analysis of barbiturates.

Authors:  R H Greeley
Journal:  Clin Chem       Date:  1974-02       Impact factor: 8.327

3.  Unsaturated fatty acids of Mycobacteria.

Authors:  J G Hung; R W Walker
Journal:  Lipids       Date:  1970-08       Impact factor: 1.880

4.  Chemical characteristics and classification of nocardiae.

Authors:  M P Lechevalier; H Lechevalier; A C Horan
Journal:  Can J Microbiol       Date:  1973-08       Impact factor: 2.419

5.  A co-operative numerical analysis of rapidly growing mycobacteria.

Authors:  G P Kubica; I Baess; R E Gordon; P A Jenkins; J B Kwapinski; C McDurmont; S R Pattyn; H Saito; V Silcox; J L Stanford; K Takeya; M Tsukamura
Journal:  J Gen Microbiol       Date:  1972-11

6.  [Attempt at specific inhibition of the growth of tuberculosis bacilli. I. Inhibition of the synthesis of phthienoic and mycocerosic acids].

Authors:  P Bennet; J Asselineau
Journal:  Ann Inst Pasteur (Paris)       Date:  1968-04

7.  Differentiation between Mycobacterium kansasii and Mycobacterium marinum by gas-liquid chromatographic analysis of cellular fatty acids.

Authors:  C O Thoen; A G Karlson; R D Ellefson
Journal:  Appl Microbiol       Date:  1972-12

8.  Profile analysis of total mycolic acids from skin corynebacteria and from named Corynebacterium strains by gas-liquid chromatography and gas-liquid chromatography/mass spectrometry.

Authors:  D L Corina; D Sesardic
Journal:  J Gen Microbiol       Date:  1980-01

9.  Comparison of rapid methods for analysis of bacterial fatty acids.

Authors:  C W Moss; M A Lambert; W H Merwin
Journal:  Appl Microbiol       Date:  1974-07

10.  Lipid composition in the classification of nocardiae and mycobacteria.

Authors:  M P Lechevalier; A C Horan; H Lechevalier
Journal:  J Bacteriol       Date:  1971-01       Impact factor: 3.490
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  20 in total

1.  Direct identification of Mycobacterium species in Bactec 7H12B medium by gas-liquid chromatography.

Authors:  S Chou; P Chedore; A Haddad; N R Paul; S Kasatiya
Journal:  J Clin Microbiol       Date:  1996-05       Impact factor: 5.948

2.  Analysis of mycolic acid cleavage products and cellular fatty acids of Mycobacterium species by capillary gas chromatography.

Authors:  M A Lambert; C W Moss; V A Silcox; R C Good
Journal:  J Clin Microbiol       Date:  1986-04       Impact factor: 5.948

3.  Burden of unidentifiable mycobacteria in a reference laboratory.

Authors:  E Tortoli; A Bartoloni; E C Böttger; S Emler; C Garzelli; E Magliano; A Mantella; N Rastogi; L Rindi; C Scarparo; P Urbano
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

4.  Wild animal mycobacterial isolates. Characterization by cellular fatty acid composition and polar lipid patterns.

Authors:  F Saxegaard; O Andersen; E Jantzen
Journal:  Acta Vet Scand       Date:  1983       Impact factor: 1.695

5.  Requirement of the mymA operon for appropriate cell wall ultrastructure and persistence of Mycobacterium tuberculosis in the spleens of guinea pigs.

Authors:  Amit Singh; Radhika Gupta; R A Vishwakarma; P R Narayanan; C N Paramasivan; V D Ramanathan; Anil K Tyagi
Journal:  J Bacteriol       Date:  2005-06       Impact factor: 3.490

6.  Chemotypes of Mycobacterium malmoense based on glycolipid profiles.

Authors:  M L Katila; E Brander; E Jantzen; R Huttunen; L Linkosalo
Journal:  J Clin Microbiol       Date:  1991-02       Impact factor: 5.948

7.  Gas chromatographic fatty acid profiles for characterisation of mycobacteria: an interlaboratory methodological evaluation.

Authors:  L Larsson; E Jantzen; J Johnsson
Journal:  Eur J Clin Microbiol       Date:  1985-10       Impact factor: 3.267

8.  Clinical significance, biochemical features, and susceptibility patterns of sporadic isolates of the Mycobacterium chelonae-like organism.

Authors:  R J Wallace; V A Silcox; M Tsukamura; B A Brown; J O Kilburn; W R Butler; G Onyi
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

Review 9.  Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s.

Authors:  Enrico Tortoli
Journal:  Clin Microbiol Rev       Date:  2003-04       Impact factor: 26.132

10.  Characterization of Mycobacterium paratuberculosis by gas-liquid and thin-layer chromatography and rapid demonstration of mycobactin dependence using radiometric methods.

Authors:  J J Damato; C Knisley; M T Collins
Journal:  J Clin Microbiol       Date:  1987-12       Impact factor: 5.948
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