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Literature DB >> 4630605

Serological detection of enterotoxin in foods implicated in staphylococcal food poisoning.

R J Gilbert, A A Wieneke, J Lanser, M Simkovicová.

Abstract

Two methods are described for the extraction of enterotoxin from foods incriminated in incidents of staphylococcal food poisoning. Enterotoxin was detected serologically in 12 of 24 food samples from 20 separate incidents: eight samples contained enterotoxin A, three contained D and one both A and B. The amount of enterotoxin in nine foods, based on 100% recovery, varied from 0.02 to 0.09 mug./g.Data are also given on the numbers of Staphylococcus aureus isolated from samples of food from 39 food poisoning incidents. Colony counts varied between 7.5 x 10(5) and 9 x 10(9)/g. with a median value of 7 x 10(7)/g.

Entities: Disease Gene Species

Mesh：

Substances：
Enterotoxins

Year: 1972 PMID： 4630605 PMCID： PMC2130284 DOI： 10.1017/s0022172400022592

Source DB: PubMed Journal: J Hyg (Lond) ISSN： 0022-1724

11 in total

9. Use of phenolphthale in diphosphate agar with polymyxin as a selective medium for the isolation and enumeration of coagulase-positive staphylococci from foods.

Authors: B C Hobbs; M Kendall; R J Gilbert
Journal: Appl Microbiol Date: 1968-03

3. The use of a sandwich ELISA for the detection of staphylococcal enterotoxin A in foods from outbreaks of food poisoning.

Authors: A A Wieneke; R J Gilbert
Journal: J Hyg (Lond) Date: 1985-08

Review 7. Methicillin-resistant Staphylococcus aureus: a pervasive pathogen highlights the need for new antimicrobial development.

Authors: Emily A Morell; Daniel M Balkin
Journal: Yale J Biol Med Date: 2010-12

7 in total

Serological detection of enterotoxin in foods implicated in staphylococcal food poisoning.

1. DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD.

2. DETECTION OF THE STAPHYLOCOCCAL ENTEROTOXINS IN FOOD.

3. Factors affecting the secretion of staphylococcal enterotoxin A.

4. The micro-slide gel double diffusion test for the detection and assay of staphylococcal enterotoxins.

5. Staphylococcal food poisoning.

6. Enterotoxin B synthesis by replicating and nonreplicating cells of Staphylococcus aureus.

7. Regulation of staphylococcal enterotoxin B.

8. Identification of a fourth staphylococcal enterotoxin, enterotoxin D.

9. Use of phenolphthale in diphosphate agar with polymyxin as a selective medium for the isolation and enumeration of coagulase-positive staphylococci from foods.

10. Effect of enterotoxin B on human volunteers.

1. Comparison of different purification procedure for extraction of staphylococcal enterotoxin A from foods.

2. Staphylococcal food poisoning and botulism.

3. The use of a sandwich ELISA for the detection of staphylococcal enterotoxin A in foods from outbreaks of food poisoning.

4. Minimal water activity for enterotoxin A production and growth of Staphylococcus aureus.

5. Identification of enterotoxigenic staphylococci from sheep and sheep cheese.

6. Staphylococcal food poisoning in the United Kingdom, 1969-90.

Review 7. Methicillin-resistant Staphylococcus aureus: a pervasive pathogen highlights the need for new antimicrobial development.