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Literature DB >> 4615633

Factors affecting the sensitivity of Limulus lysate.

Abstract

Limulus lysate clots when mixed with picogram quantities of endotoxins. The sensitivity of the lysate was improved 100-fold by the removal of an inhibitor and addition of divalent cations. The methods developed in this investigation eliminated much of the seasonable variability of the lysate, improved the heat stability after lyophilization, and made it possible to use the lysate with saline solutions.

Entities: Chemical

Mesh：

Substances：

Year: 1974 PMID： 4615633 PMCID： PMC186875 DOI： 10.1128/am.28.6.1023-1026.1974

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

14 in total

1. THE ROLE OF ENDOTOXIN IN THE EXTRACELLULAR COAGULATION OF LIMULUS BLOOD.

Authors: J LEVIN; F B BANG
Journal: Bull Johns Hopkins Hosp Date: 1964-09

2. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors: B J DAVIS
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

3. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.

Authors: L ORNSTEIN
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

4. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.

Authors: A Chrambach; R A Reisfeld; M Wyckoff; J Zaccari
Journal: Anal Biochem Date: 1967-07 Impact factor: 3.365

5. Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay.

Authors: J H Jorgensen; R F Smith
Journal: Appl Microbiol Date: 1973-07

6. A technique for quantitative measurement of endotoxin in human plasma.

Authors: R B Reinhold; J Fine
Journal: Proc Soc Exp Biol Med Date: 1971-05

7. Gram-negative sepsis: detection of endotoxemia with the limulus test. With studies of associated changes in blood coagulation, serum lipids, and complement.

Authors: J Levin; T E Poore; N S Young; S Margolis; N P Zauber; A S Townes; W R Bell
Journal: Ann Intern Med Date: 1972-01 Impact factor: 25.391

8. Detection of endotoxin in human blood and demonstration of an inhibitor.

Authors: J Levin; P A Tomasulo; R S Oser
Journal: J Lab Clin Med Date: 1970-06

9. Some characteristics of the clottable protein of limulus polyphemus blood cells.

Authors: N O Solum
Journal: Thromb Diath Haemorrh Date: 1970-02-28

10. Rapid detection of gram-negative bacterial meningitis by the limulus lysate test.

Authors: R Nachum; A Lipsey; S E Siegel
Journal: N Engl J Med Date: 1973-11-01 Impact factor: 91.245

26 in total

1. Influence of substrate wettability on the attachment of marine bacteria to various surfaces.

Authors: S C Dexter; J D Sullivan; J Williams; S W Watson
Journal: Appl Microbiol Date: 1975-08

2. Rapid determination of bacteriological water quality by using limulus lysate.

Authors: T M Evans; J E Schillinger; D G Stuart
Journal: Appl Environ Microbiol Date: 1978-02 Impact factor: 4.792

3. Acute inhalation toxicity of cotton plant dusts.

Authors: R Rylander; M C Snella
Journal: Br J Ind Med Date: 1976-08

4. Purification of specific precipitinogen and extraction of endotoxin from Haemophilus influenzae.

Authors: J C van der Zwan; J Dankert; K de Vries; N G Orie; H F Kauffman
Journal: J Clin Pathol Date: 1978-04 Impact factor: 3.411

5. Single-step, chromogenic Limulus amebocyte lysate assay for endotoxin.

Authors: G K Lindsay; P F Roslansky; T J Novitsky
Journal: J Clin Microbiol Date: 1989-05 Impact factor: 5.948

6. Very slow growth of Escherichia coli.

Authors: W Chesbro; T Evans; R Eifert
Journal: J Bacteriol Date: 1979-08 Impact factor: 3.490

7. Immunochemical characterization of rough Brucella lipopolysaccharides.

Authors: E Moreno; L M Jones; D T Berman
Journal: Infect Immun Date: 1984-03 Impact factor: 3.441

8. Endotoxic activity of rough organisms of Brucella species.

Authors: L M Jones; R Diaz; D T Berman
Journal: Infect Immun Date: 1976-06 Impact factor: 3.441

9. Rapid detection of bacterial endotoxins in drinking water and renovated wastewater.

Authors: J H Jorgensen; J C Lee; H R Pahren
Journal: Appl Environ Microbiol Date: 1976-09 Impact factor: 4.792

10. Inhibitory effect of heparin on the Limulus test for endotoxin.

Authors: J D Sullivan; S W Watson
Journal: J Clin Microbiol Date: 1976-08 Impact factor: 5.948