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Literature DB >> 4199336

Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay.

Abstract

Limulus amoebocyte lysate was prepared from a total of 180 crabs during 1971 and 1972 by using a slightly modified lysate preparation procedure. Marked variability of lysate potency was noted both years. In addition, lysate quality appeared diminished in 1972 as compared with 1971. Different lysate batches were evaluated for potency by using a variety of endotoxin preparations. Variations in batch potencies were observed, but little variation in reactivity among different endotoxin preparations was noted. Use of potent lysate batches allowed detection of endotoxin concentrations as low as 100 pg/ml. No endotoxinlike activity was observed from 11 different strains of yeast by use of the Limulus assay.

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Year: 1973 PMID： 4199336 PMCID： PMC379714 DOI： 10.1128/am.26.1.43-48.1973

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

12 in total

1. Detection of lipopolysaccharide (LPS): an improved method for isolation of the Limulus extract.

Authors: P A Ward; J H Hill
Journal: Proc Soc Exp Biol Med Date: 1972-12

2. Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria.

Authors: E T Yin; C Galanos; S Kinsky; R A Bradshaw; S Wessler; O Lüderitz; M E Sarmiento
Journal: Biochim Biophys Acta Date: 1972-01-28

3. Role of intraintestinal endotoxin in death from peritonitis.

Authors: P Cuevas; J Fine
Journal: Surg Gynecol Obstet Date: 1972-06

4. Endotoxaemia in man.

Authors: D T Caridis; R B Reinhold; P W Woodruff; J Fine
Journal: Lancet Date: 1972-06-24 Impact factor: 79.321

5. A technique for quantitative measurement of endotoxin in human plasma.

Authors: R B Reinhold; J Fine
Journal: Proc Soc Exp Biol Med Date: 1971-05

6. Quantitative comparison of in vitro and in vivo methods for the detection of endotoxin.

Authors: J F Cooper; J Levin; H N Wagner
Journal: J Lab Clin Med Date: 1971-07

7. The Limulus test for endotoxin (Pyrogen) in radiopharmaceuticals and biologicals.

Authors: J F Cooper; H D Hochstein; E B Seligmann
Journal: Bull Parenter Drug Assoc Date: 1972 Jul-Aug

8. Clottable protein in Limulus; its localization and kinetics of its coagulation by endotoxin.

Authors: J Levin; F B Bang
Journal: Thromb Diath Haemorrh Date: 1968-03-31

9. Preparation of a purified lipopolysaccharide for pyrogen testing.

Authors: G B Selzer
Journal: Bull Parenter Drug Assoc Date: 1970 May-Jun

10. An invertebrate coagulation system activated by endotoxin: evidence for enzymatic mediation.

Authors: N S Young; J Levin; R A Prendergast
Journal: J Clin Invest Date: 1972-07 Impact factor: 14.808

27 in total

1. Bacteriological activity in unfiltered calf sera collected for tissue culture use.

Authors: H C Orr; K H Sibinovic; P G Probst; H D Hochstein; D C Littlejohn
Journal: In Vitro Date: 1975 Jul-Aug

2. Rapid determination of bacteriological water quality by using limulus lysate.

Authors: T M Evans; J E Schillinger; D G Stuart
Journal: Appl Environ Microbiol Date: 1978-02 Impact factor: 4.792

3. Opsonizing antibodies, host factors, and the limulus assay for endotoxin.

Authors: L S Young
Journal: Infect Immun Date: 1975-07 Impact factor: 3.441

4. Purification of specific precipitinogen and extraction of endotoxin from Haemophilus influenzae.

Authors: J C van der Zwan; J Dankert; K de Vries; N G Orie; H F Kauffman
Journal: J Clin Pathol Date: 1978-04 Impact factor: 3.411

5. Comparison of Limulus assay, standard plate count, and total coliform count for microbiological assessment of renovated wastewater.

Authors: J H Jorgensen; J C Lee; G A Alexander; H W Wolf
Journal: Appl Environ Microbiol Date: 1979-05 Impact factor: 4.792

6. Comparison of lipopolysaccharides from Agmenellum quadruplicatum to Escherichia coli and Salmonella typhimurium by using thin-layer chromatography.

Authors: T M Buttke; L O Ingram
Journal: J Bacteriol Date: 1975-12 Impact factor: 3.490

10. Acute lung inflammation induced in the rabbit by local instillation of 1-0-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine or of native platelet-activating factor.

Authors: G Camussi; I Pawlowski; C Tetta; C Roffinello; M Alberton; J Brentjens; G Andres
Journal: Am J Pathol Date: 1983-07 Impact factor: 4.307