Literature DB >> 305926

Purification of specific precipitinogen and extraction of endotoxin from Haemophilus influenzae.

J C van der Zwan, J Dankert, K de Vries, N G Orie, H F Kauffman.   

Abstract

After purifying a Haemophilus influenzae precipitinogen from endotoxic activity by means of ultracentrifugation, column chromatography (Sepharose 6B) and ion exchange chromatography (DEAE Sephadex A25) a fraction was obtained which still contained a specific precipitinogen that was virtually free of endotoxin. Furthermore, during the chromatographic procedures fractions with a high and a low molecular weight endotoxic activity were found. The limulus lysate test was more sensitive in the high molecular weight fractions and the LD50 in mice in the low molecular weight fractions with endotoxic activity.

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Year:  1978        PMID： 305926      PMCID： PMC1145278          DOI： 10.1136/jcp.31.4.370

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  36 in total

1.  CARBOHYDRATE ANALYSIS OF BACTERIAL SUBSTANCES BY A NEW ANTHRONE PROCEDURE.

Authors:  G TOENNIES; J J KOLB
Journal:  Anal Biochem       Date:  1964-05       Impact factor: 3.365

2.  [EARLY AND LATE REACTIONS WITH BACTERIAL ALLERGENS IN BRONCHIAL ASTHMA].

Authors:  K HAJOS; M K HAJOS
Journal:  Allerg Asthma (Leipz)       Date:  1964

3.  A DOUBLE-BLIND STUDY OF THE EFFECTS OF BACTERIAL VACCINE ON INFECTIVE ASTHMA.

Authors:  S E BARR; H BROWN; M FUCHS; H ORVIS; A CONNOR; F J MURRAY; A SELTZER
Journal:  J Allergy       Date:  1965 Jan-Feb

4.  An evaluation of sputum examination in chronic bronchitis.

Authors:  W BRUMFITT; M L WILLOUGHBY; L L BROMLEY
Journal:  Lancet       Date:  1957-12-28       Impact factor: 79.321

5.  Diffusion-in-gel methods for immunological analysis. II.

Authors:  O OUCHTERLONY
Journal:  Prog Allergy       Date:  1962

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

7.  Bronchial hypersensitization and hyposensitization in man.

Authors:  H HERXHEIMER
Journal:  Int Arch Allergy Appl Immunol       Date:  1951

8.  Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers.

Authors:  S De Petris
Journal:  J Ultrastruct Res       Date:  1967-07

9.  Comparison of the chemical structure and biological activities of the glycolipids of Salmonella minnesota R595 and Salmonella typhimurium SL1102.

Authors:  A K Ng; C M Chang; C H Chen; A Nowotny
Journal:  Infect Immun       Date:  1974-10       Impact factor: 3.441

10.  SEROLOGICAL STUDIES ON HAEMOPHILUS INFLUENZAE AND RELATED SPECIES. 8. EXAMINATIONS OF ULTRASONICALLY PREPARED HAEMOPHILUS ANTIGENS BY MEANS OF IMMUNO-ELECTROPHORESIS.

Authors:  T OMLAND
Journal:  Acta Pathol Microbiol Scand       Date:  1964
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