Literature DB >> 450695

A precise quantitation of gene number by saturation hybridization using cloned DNA.

S S Longacre, B Mach.   

Abstract

This paper describes a precise method of gene titration as applied to the alpha- and beta-globin genes in the mouse. The three salient features of the method are: (i) the use of saturation hybridization in probe cDNA excess, (ii) the use of highly purified cDNA probes prepared by preparative hybridization with cloned globin sequences (Longacre and Mach (1978) J. Biol. Chem. 253, 7500) and (iii) the use of cloned globin sequences to calibrate the system internally. The results indicate that there are two genes for alpha-globin and two genes for beta-globin in the BALB/c mouse. The significance of these results are discussed in relation to other data regarding adult and embryonic globin genes.

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Year:  1979        PMID： 450695      PMCID： PMC327770          DOI： 10.1093/nar/6.4.1241

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

Review 1.  Summary: the molecular biology of the eukaryotic genome is coming of age.

Authors:  P Chambon
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978

2.  Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences.

Authors:  S M Tilghman; D C Tiemeier; F Polsky; M H Edgell; J G Seidman; A Leder; L W Enquist; B Norman; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

3.  Intervening sequence of DNA identified in the structural portion of a mouse beta-globin gene.

Authors:  S M Tilghman; D C Tiemeier; J G Seidman; B M Peterlin; M Sullivan; J V Maizel; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

4.  Reduction in the rate of DNA reassociation by sequence divergence.

Authors:  T I Bonner; D J Brenner; B R Neufeld; R J Britten
Journal:  J Mol Biol       Date:  1973-12-05       Impact factor: 5.469

5.  The sequence of the chromosomal mouse beta-globin major gene: homologies in capping, splicing and poly(A) sites.

Authors:  D A Konkel; S M Tilghman; P Leder
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

6.  Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.

Authors:  F Rougeon; P Kourilsky; B Mach
Journal:  Nucleic Acids Res       Date:  1975-12       Impact factor: 16.971

7.  Purification of specific DNA sequences by sulfhydryl-Sepharose chromatography of mercurated polynucleotides.

Authors:  S S Longacre; B Mach
Journal:  J Biol Chem       Date:  1978-10-25       Impact factor: 5.157

8.  Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.

Authors:  L Katz; D T Kingsbury; D R Helinski
Journal:  J Bacteriol       Date:  1973-05       Impact factor: 3.490

9.  Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro.

Authors:  F Rougeon; B Mach
Journal:  Gene       Date:  1977-05       Impact factor: 3.688

10.  The intervening sequence of a mouse beta-globin gene is transcribed within the 15S beta-globin mRNA precursor.

Authors:  S M Tilghman; P J Curtis; D C Tiemeier; P Leder; C Weissmann
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205
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