| Literature DB >> 338418 |
Abstract
New chimeric Escherichia coli plasmids containing alpha or beta globin gene sequences of the mouse were constructed. Double-stranded DNA, synthesised in vitro in a 2-step reaction from mouse globin mRNA was inserted into E. coli plasmid pCR1, after tailing of the 2 DNAs with dG and dC respectively. Some of the mouse globin plasmids described contain at least 90% of the globin mRNA sequence and therefore contain the entire translated sequence of the globin genes. Some possible uses of these recombinant plasmids are described.Entities:
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Year: 1977 PMID： 338418 DOI： 10.1016/0378-1119(77)90047-6
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688