Literature DB >> 270684

Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences.

S M Tilghman, D C Tiemeier, F Polsky, M H Edgell, J G Seidman, A Leder, L W Enquist, B Norman, P Leder.   

Abstract

We have developed a general approach to the cloning of specific segments of the mammalian genome that involves a two-step purification of EcoRI fragments of mammalian DNA and their in vitro insertion into a suitably constructed EK2 derivative of bacteriophage lambda. The combination of fragment purification, exclusion of parental-type recombinants, and simple phage screening techniques permits the isolation of virtually any gene segment for which there is an identifying hybridization probe. We illustrate the approach by describing the cloning of an approximately 7000-base-long segment of mouse DNA containing globin and surrounding gene sequences.

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Year:  1977        PMID： 270684      PMCID： PMC431951          DOI： 10.1073/pnas.74.10.4406

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  Hemoglobin inheritance in inbred mouse strains. II. Genetic studies.

Authors:  J J HUTTON; J BISHOP; R SCHWEET; E S RUSSELL
Journal:  Proc Natl Acad Sci U S A       Date:  1962-10-15       Impact factor: 11.205

2.  Preparative fractionation of DNA restriction fragments by reversed phase column chromatography.

Authors:  S C Hardies; R D Wells
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205

3.  Improved derivative of a phage lambda EK2 vector for cloning recombinant DNA.

Authors:  D Tiemer; L Enquist; P Leder
Journal:  Nature       Date:  1976-10-07       Impact factor: 49.962

4.  Preparative fractionation of DNA restriction fragments by high pressure column chromatography on RPC-5.

Authors:  A Landy; C Foeller; R Reszelbach; B Dudock
Journal:  Nucleic Acids Res       Date:  1976-10       Impact factor: 16.971

5.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

6.  A comparison of globin genes in duck reticulocytes and liver cells.

Authors:  S Packman; H Aviv; J Ross; P Leder
Journal:  Biochem Biophys Res Commun       Date:  1972-11-01       Impact factor: 3.575

7.  The primary structure of rabbit beta-globin mRNA as determined from cloned DNA.

Authors:  A Efstratiadis; F C Kafatos; T Maniatis
Journal:  Cell       Date:  1977-04       Impact factor: 41.582

8.  Isolation of bacteriophage lambda containing yeast ribosomal RNA genes: screening by in situ RNA hybridization to plaques.

Authors:  R A Kramer; J R Cameron; R W Davis
Journal:  Cell       Date:  1976-06       Impact factor: 41.582

9.  Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro.

Authors:  F Rougeon; B Mach
Journal:  Gene       Date:  1977-05       Impact factor: 3.688

10.  Nucleotide sequence of the rightward operator of phage lambda.

Authors:  T Maniatis; A Jeffrey; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205
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  69 in total

1.  Cloning of immunoglobulin kappa light chain genes from mouse liver and myeloma MOPC 173.

Authors:  M Steinmetz; H G Zachau; B Mach
Journal:  Nucleic Acids Res       Date:  1979-07-25       Impact factor: 16.971

2.  Structural studies on oncornavirus-related sequences in chicken genomic DNA: two-step analyses of EcoRI and Bgl I restriction digests and tentative mapping of a ubiquitous endogenous provirus digests and tentative mapping of a ubiquitous endogenous provirus.

Authors:  W McClements; H Hanafusa; S Tilghman; A Skalka
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

Review 3.  Multiple forms of inducible drug-metabolizing enzymes: a reasonable mechanism by which any organism can cope with adversity.

Authors:  D W Nebert
Journal:  Mol Cell Biochem       Date:  1979-09-28       Impact factor: 3.396

4.  The 2007 Genetics Society of America Medal. Shirley Tilghman.

Authors:  Steve Buratowski
Journal:  Genetics       Date:  2007-02       Impact factor: 4.562

5.  Bipartite structure of the downstream element of the mouse beta globin (major) poly(A) signal.

Authors:  J S Chen; J L Nordstrom
Journal:  Nucleic Acids Res       Date:  1992-05-25       Impact factor: 16.971

6.  Truncated forms of PspA that are secreted from Streptococcus pneumoniae and their use in functional studies and cloning of the pspA gene.

Authors:  J Yother; G L Handsome; D E Briles
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

7.  Genetic analysis of the 9.5-kilobase virulence plasmid of Yersinia pestis.

Authors:  O A Sodeinde; J D Goguen
Journal:  Infect Immun       Date:  1988-10       Impact factor: 3.441

8.  Mice lacking N-acetylglucosaminyltransferase I activity die at mid-gestation, revealing an essential role for complex or hybrid N-linked carbohydrates.

Authors:  E Ioffe; P Stanley
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-18       Impact factor: 11.205

9.  Specific differentiation events induced by erythropoietin in cells infected in vitro with the anemia strain of Friend virus.

Authors:  M J Koury; M C Bondurant; D T Duncan; S B Krantz; W D Hankins
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

10.  Structure of genes for human growth hormone and chorionic somatomammotropin.

Authors:  J C Fiddes; P H Seeburg; F M DeNoto; R A Hallewell; J D Baxter; H M Goodman
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205
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