Literature DB >> 4361675

A mammalian cell mutant with a temperature-sensitive leucyl-transfer RNA synthetase.

L H Thompson, J L Harkins, C P Stanners.   

Abstract

A cell mutant of the Chinese hamster ovary line, which is temperature sensitive for protein synthesis, is specifically defective in vivo in its ability to charge tRNA with leucine. Cytoplasmic extracts exhibited temperature-sensitive leucyl-tRNA synthetase activity. It is, therefore, highly likely that the mutant has a structural alteration in leucyl-tRNA synthetase. The low leakiness and low reversion rate of this mutant, combined with the specificity of the defect in its protein-synthesizing machinery, make it an appealing tool for investigating regulatory mechanisms in animal cells.

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Year:  1973        PMID： 4361675      PMCID： PMC427177          DOI： 10.1073/pnas.70.11.3094

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  Mutation rates in cells at different ploidy levels.

Authors:  M Harris
Journal:  J Cell Physiol       Date:  1971-10       Impact factor: 6.384

2.  Polyribosomes of hamster cells: transit time measurements.

Authors:  C P Stanners
Journal:  Biophys J       Date:  1968-02       Impact factor: 4.033

3.  Control of macromolecular synthesis in proliferating and resting Syrian hamster cells in monolayer culture. I. Ribosome function.

Authors:  C P Stanners; H Becker
Journal:  J Cell Physiol       Date:  1971-02       Impact factor: 6.384

4.  Two types of ribosome in mouse-hamster hybrid cells.

Authors:  C P Stanners; G L Eliceiri; H Green
Journal:  Nat New Biol       Date:  1971-03-10

5.  Isolation of temperature-sensitive mutants of L-cells.

Authors:  L H Thompson; R Mankovitz; R M Baker; J E Till; L Siminovitch; G F Whitmore
Journal:  Proc Natl Acad Sci U S A       Date:  1970-06       Impact factor: 11.205

6.  Transfer RNA species in normal and leukemic human lymphoblasts.

Authors:  R C Gallo; S Pestka
Journal:  J Mol Biol       Date:  1970-09-14       Impact factor: 5.469

7.  A role of aminoacyl-tRNA in the regulation of protein breakdown in Escherichia coli.

Authors:  A L Goldberg
Journal:  Proc Natl Acad Sci U S A       Date:  1971-02       Impact factor: 11.205

8.  Temperature sensitive conditional mutants of monkey kidney cells.

Authors:  P M Naha
Journal:  Nature       Date:  1969-09-27       Impact factor: 49.962

9.  Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases.

Authors:  B Low; F Gates; T Goldstein; D Söll
Journal:  J Bacteriol       Date:  1971-11       Impact factor: 3.490

10.  Controlled production of proliferating somatic cell hybrids.

Authors:  R J Klebe; T Chen; F H Ruddle
Journal:  J Cell Biol       Date:  1970-04       Impact factor: 10.539
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  28 in total

1.  Temperature-sensitive Chinese hamster fibroblast mutant with a defect in RNA metabolism.

Authors:  E A Wong; I E Scheffler
Journal:  Mol Cell Biol       Date:  1982-12       Impact factor: 4.272

2.  Isolation of cold-sensitive Chinese hamster cells.

Authors:  R A Farber; P Unrau
Journal:  Mol Gen Genet       Date:  1975-06-19

3.  Temperature-sensitive breakdown in vivo of polysomes with mutants of Chlamydomonas reinhardii.

Authors:  J Cross; D McMahon
Journal:  Mol Gen Genet       Date:  1976-08-19

4.  Assignment of a gene for tryptophanyl-transfer ribonucleic acid synthetase (E.C. 6.1.1.2) to human chromosome 14.

Authors:  R M Denney; I W Craig
Journal:  Biochem Genet       Date:  1976-02       Impact factor: 1.890

5.  Deletion mapping of human chromosome 5 using chromosome-specific DNA probes.

Authors:  L R Carlock; D Skarecky; S L Dana; J J Wasmuth
Journal:  Am J Hum Genet       Date:  1985-09       Impact factor: 11.025

6.  Identification of temperature-sensitive DNA- mutants of Chinese hamster cells affected in cellular and viral DNA synthesis.

Authors:  J J Dermody; B E Wojcik; H Du; H L Ozer
Journal:  Mol Cell Biol       Date:  1986-12       Impact factor: 4.272

7.  Stuttering: high-level mistranslation in animal and bacterial cells.

Authors:  J Parker; J W Pollard; J D Friesen; C P Stanners
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

8.  Deleted chromosome 20 from a patient with Alagille syndrome isolated in a cell hybrid through leucine transport selection: study of three candidate genes.

Authors:  J F Deleuze; S Dhorne; J Hazan; E Borghi; N Raynaud; N Pollet; M Meunier-Rotival; J Deschatrette; D Alagille; M Hadchouel
Journal:  Mamm Genome       Date:  1994-11       Impact factor: 2.957

9.  Quantities of individual aminoacyl-tRNA families and their turnover in Escherichia coli.

Authors:  H Jakubowski; E Goldman
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490

10.  Re-evaluating the roles of proposed modulators of mammalian target of rapamycin complex 1 (mTORC1) signaling.

Authors:  Xuemin Wang; Bruno D Fonseca; Hua Tang; Rui Liu; Androulla Elia; Michael J Clemens; Ulrich-Axel Bommer; Christopher G Proud
Journal:  J Biol Chem       Date:  2008-08-01       Impact factor: 5.157
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