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Literature DB >> 1237784

Isolation of cold-sensitive Chinese hamster cells.

Abstract

Six cold-sensitive variants have been isolated from Chinese hamster ovary cells by the BUdR-visible light selection technique. The properties of one of these lines have been studied in detail. This line stops dividing immediately after a shift from 39 degrees C to 33 degrees C though its doubling time at 39 degrees C is only slightly longer than that of wild-type cells. The rates of DNA and protein synthesis are severely reduced at 33 degrees C, but the rate of RNA synthesis is not significantly different from wild-type cells. This line may be defective in protein synthesis, but the results of sedimentation analysis indicate that it probably has normal ribosomal subunit assembly.

Entities: Species

Mesh：

Substances：
RNA
DNA
Bromodeoxyuridine

Year: 1975 PMID： 1237784 DOI： 10.1007/bf00269350

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

38 in total

1. Cold sensitive mutant of Escherichia coli with altered RNA polymerase.

Authors: V G Nikiforov; E S Kalyaeva; V V Velkov
Journal: Mol Gen Genet Date: 1974-04-09

2. A temperature-sensitive function in a Chinese hamster line affecting DNA synthesis.

Authors: B J Smith; N M Wigglesworth
Journal: J Cell Physiol Date: 1973-12 Impact factor: 6.384

3. Conditionally lethal mutations in Chinese hamster cells. I. Isolation of a temperature-sensitive line and its investigation by cell cycle studies.

Authors: I E Scheffler; G Buttin
Journal: J Cell Physiol Date: 1973-04 Impact factor: 6.384

8. Mutation in Saccharomyces cerevisiae conferring streptomycin and cold sensitivity by affecting ribosome formation and function.

Authors: F T Bayliss; J L Ingrahm
Journal: J Bacteriol Date: 1974-05 Impact factor: 3.490

Isolation of cold-sensitive Chinese hamster cells.

1. Cold sensitive mutant of Escherichia coli with altered RNA polymerase.

2. A temperature-sensitive function in a Chinese hamster line affecting DNA synthesis.

3. Conditionally lethal mutations in Chinese hamster cells. I. Isolation of a temperature-sensitive line and its investigation by cell cycle studies.

4. Rate of DNA synthesis as a function of temperature in cultured hamster fibroblasts (V-79) and HeLa-S3 cells.

5. A new class of temperature conditional lethal mutants of bacteriophage T4D.

6. Cold-sensitive mutations in Salmonella typhimurium which affect ribosome synthesis.

7. Temperature sensitive conditional mutants of monkey kidney cells.

8. Mutation in Saccharomyces cerevisiae conferring streptomycin and cold sensitivity by affecting ribosome formation and function.

9. The regulation of RNA synthesis and processing in the nucleolus during inhibition of protein synthesis.

10. Puromycin resistance in haploid and heteroploid frog cells: gene or membrane determined?

1. Molecular recombination and the repair of DNA double-strand breaks in CHO cells.

2. Cold-sensitive growth of simian virus 40 in semipermissive variants of CV1 cells.

3. An enrichment for temperature-sensitive mutants in Tetrahymena thermophila.