Literature DB >> 4358863

Evaluation and modifications of media for enumeration of Clostridium perfringens.

A H Hauschild, R Hilsheimer.   

Abstract

The suitability of the Shahidi-Ferguson perfringens, TSC (tryptose-sulfite-cycloserine), and oleandomycin-polymyxin-sulfadiazine perfringens agars for presumptive enumeration of Clostridium perfringens was tested. Of these, the TSC agar was the most satisfactory. The TSC agar method was improved by eliminating the egg yolk and using pour plates. The modified method allowed quantitative recoveries of each of 71 C. perfringens strains tested and is recommended. For confirmation of C. perfringens, the nitrite test in nitrate motility agar was unreliable, particularly after storage of the medium for a few days. In contrast, positive nitrite reactions were obtained consistently when nitrate motility agar was supplemented with glycerol and galactose.

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Year:  1974        PMID： 4358863      PMCID： PMC379971          DOI： 10.1128/am.27.1.78-82.1974

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  9 in total

1.  EFFECT OF PH ON TOXINOGENESIS BY CLOSTRIDIUM PERFRINGENS TYPE C.

Authors:  H PIVNICK; A F HABEEB; B GORENSTEIN; P F STUART; A H HAUSCHILD
Journal:  Can J Microbiol       Date:  1964-06       Impact factor: 2.419

2.  Rapid destruction of bacteria in commonly used diluents and its elimination.

Authors:  R P STRAKA; J L STOKES
Journal:  Appl Microbiol       Date:  1957-01

3.  New selective medium for the isolation of Clostridium perfringens.

Authors:  M Füzi; Z Csukás
Journal:  Acta Microbiol Acad Sci Hung       Date:  1969

4.  Studies on the suitability of sulphite cycloserine agar for the enumeration of clostridium perfringens in food and water.

Authors:  D A Mossel; H Pouw
Journal:  Zentralbl Bakteriol Orig A       Date:  1973-05

5.  Quantitation of Clostridium perfringens in foods.

Authors:  R ANGELOTTI; H E HALL; M J FOTER; K H LEWIS
Journal:  Appl Microbiol       Date:  1962-05

6.  New quantitative, qualitative, and confirmatory media for rapid analysis of food for Clostridium perfringens.

Authors:  S A Shahidi; A R Ferguson
Journal:  Appl Microbiol       Date:  1971-03

7.  Comparison of media for the enumeration of Clostridium perfringens.

Authors:  S M Harmon; D A Kautter; J T Peeler
Journal:  Appl Microbiol       Date:  1971-05

8.  RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS.

Authors:  R S MARSHALL; J F STEENBERGEN; L S MCCLUNG
Journal:  Appl Microbiol       Date:  1965-07

9.  Improved medium for enumeration of Clostridium perfringens.

Authors:  S M Harmon; D A Kautter; J T Peeler
Journal:  Appl Microbiol       Date:  1971-10
  9 in total
  16 in total

1.  Comparison of sulfite-polymyxin-sulfadiazine medium and tryptose-sulfite-cycloserine medium without egg yolk for recovering Clostridium perfringens.

Authors:  D S Orth
Journal:  Appl Environ Microbiol       Date:  1977-04       Impact factor: 4.792

2.  Comparative Study of Two Methods for Detection of Clostridium perfringens in Ground Beef.

Authors:  B S Emswiler; C J Pierson; A W Kotula
Journal:  Appl Environ Microbiol       Date:  1977-03       Impact factor: 4.792

3.  Isolation and characterization of multiply antibiotic-resistant Clostridum perfringens strains from porcine feces.

Authors:  J I Rood; E A Maher; E B Somers; E Campos; C L Duncan
Journal:  Antimicrob Agents Chemother       Date:  1978-05       Impact factor: 5.191

4.  Membrane filter enumeration method for Clostridium perfringens.

Authors:  J W Bisson; V J Cabelli
Journal:  Appl Environ Microbiol       Date:  1979-01       Impact factor: 4.792

5.  New medium for rapid screening and enumeration of Clostridium perfringens in foods.

Authors:  J E Erickson; R H Deibel
Journal:  Appl Environ Microbiol       Date:  1978-10       Impact factor: 4.792

6.  The bacteriological quality of minced beef in the U.K.

Authors:  T A Roberts; C R Britton; W R Hudson
Journal:  J Hyg (Lond)       Date:  1980-10

7.  New presumptive identification test for Clostridium perfringens: reverse CAMP test.

Authors:  M V Hansen; L P Elliott
Journal:  J Clin Microbiol       Date:  1980-10       Impact factor: 5.948

8.  Comparison of media and methods for counting Clostridium perfringens in poultry meat and further-processed products.

Authors:  B W Adams; G C Mead
Journal:  J Hyg (Lond)       Date:  1980-02

9.  Faecal carriage of Clostridium perfringens.

Authors:  M F Stringer; G N Watson; R J Gilbert; J G Wallace; J E Hassall; E I Tanner; P P Webber
Journal:  J Hyg (Lond)       Date:  1985-10

10.  Effects of storage on microbial loads of two commercially important shellfish species, Crassostrea virginica and Mercenaria campechiensis.

Authors:  M A Hood; G E Ness; G E Rodrick; N J Blake
Journal:  Appl Environ Microbiol       Date:  1983-04       Impact factor: 4.792
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