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Literature DB >> 4331774

Improved medium for enumeration of Clostridium perfringens.

Abstract

An improved selective medium, Tryptose-sulfite-cycloserine (TSC) agar, for the enumeration of Clostridium perfringens is described. It consists of the same basal medium as Shahidi-Ferguson-perfringens (SFP) agar, but with 400 mug of D-cycloserine per ml substituted for the kanamycin and polymyxin. Tolerance of C. perfringens for D-cycloserine, its production of lecithinase, and its ability to reduce sulfite were used as the basis for development of this medium. Comparisons were made between TSC and SFP agars for the recovery of vegetative cells of C. perfringens by using statistical methods. The results showed that TSC allowed virtually complete recovery of most of the C. perfringens strains while inhibiting practically all facultative anaerobes tested. SFP agar allowed a slightly higher rate of recovery of C. perfringens but was found to be much less selective.

Entities: Chemical Species

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Year: 1971 PMID： 4331774 PMCID： PMC376387 DOI： 10.1128/am.22.4.688-692.1971

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

8 in total

1. Clostridium welchii food poisoning.

Authors: B C HOBBS; M E SMITH; C L OAKLEY; G H WARRACK; J C CRUICKSHANK
Journal: J Hyg (Lond) Date: 1953-03

2. The Egg Yolk Plate Reaction for the Presumptive Diagnosis of Clostridium sporogenes and Certain Species of the Gangrene and Botulinum Groups.

Authors: L S McClung; R Toabe
Journal: J Bacteriol Date: 1947-02 Impact factor: 3.490

3. New selective medium for the isolation of Clostridium perfringens.

Authors: M Füzi; Z Csukás
Journal: Acta Microbiol Acad Sci Hung Date: 1969

4. Quantitation of Clostridium perfringens in foods.

Authors: R ANGELOTTI; H E HALL; M J FOTER; K H LEWIS
Journal: Appl Microbiol Date: 1962-05

5. Serological studies of Clostridium botulinum type E and related organisms. II. Serology of spores.

Authors: H M Solomon; R K Lynt; D A Kautter; T Lilly
Journal: J Bacteriol Date: 1969-05 Impact factor: 3.490

6. New quantitative, qualitative, and confirmatory media for rapid analysis of food for Clostridium perfringens.

Authors: S A Shahidi; A R Ferguson
Journal: Appl Microbiol Date: 1971-03

7. Comparison of media for the enumeration of Clostridium perfringens.

Authors: S M Harmon; D A Kautter; J T Peeler
Journal: Appl Microbiol Date: 1971-05

8. RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS.

Authors: R S MARSHALL; J F STEENBERGEN; L S MCCLUNG
Journal: Appl Microbiol Date: 1965-07

8 in total

24 in total

1. Sensitization of Clostridium perfringens spores to heat by gamma radiation.

Authors: D E Gombas; R F Gomez
Journal: Appl Environ Microbiol Date: 1978-09 Impact factor: 4.792

2. Thermal inactivation of ileal loop-reactive Clostridium perfringens type A strains in phosphate buffer and beef gravy.

Authors: J G Bradshaw; J T Peeler; R M Twedt
Journal: Appl Environ Microbiol Date: 1977-09 Impact factor: 4.792

3. Comparison of sulfite-polymyxin-sulfadiazine medium and tryptose-sulfite-cycloserine medium without egg yolk for recovering Clostridium perfringens.

Authors: D S Orth
Journal: Appl Environ Microbiol Date: 1977-04 Impact factor: 4.792

Improved medium for enumeration of Clostridium perfringens.

1. Clostridium welchii food poisoning.

2. The Egg Yolk Plate Reaction for the Presumptive Diagnosis of Clostridium sporogenes and Certain Species of the Gangrene and Botulinum Groups.

3. New selective medium for the isolation of Clostridium perfringens.

4. Quantitation of Clostridium perfringens in foods.

5. Serological studies of Clostridium botulinum type E and related organisms. II. Serology of spores.

6. New quantitative, qualitative, and confirmatory media for rapid analysis of food for Clostridium perfringens.

7. Comparison of media for the enumeration of Clostridium perfringens.

8. RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS.

1. Sensitization of Clostridium perfringens spores to heat by gamma radiation.

2. Thermal inactivation of ileal loop-reactive Clostridium perfringens type A strains in phosphate buffer and beef gravy.

3. Comparison of sulfite-polymyxin-sulfadiazine medium and tryptose-sulfite-cycloserine medium without egg yolk for recovering Clostridium perfringens.

4. Dialysis technique for containment of microbial populations inoculated into food systems.

5. Comparative Study of Two Methods for Detection of Clostridium perfringens in Ground Beef.

Review 6. Use of bacterial spores in monitoring water quality and treatment.

7. New medium for rapid screening and enumeration of Clostridium perfringens in foods.

8. Inhibition of Clostridium perfringens by heated combinations of nitrite, sulfur, and ferrous or ferric ions.

9. Inactivation of Clostridium perfringens type A spores at ultrahigh temperatures.

10. Development of a minimal medium for Clostridium perfringens by using an anaerobic chemostat.