| Literature DB >> 14339262 |
R S MARSHALL, J F STEENBERGEN, L S MCCLUNG.
Abstract
A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of the medium. Comparisons were made between sulfite-polymyxin-sulfadiazine (SPS) agar and TSN agar at 37 and 46 C with C. perfringens and other organisms. These studies indicate the quantitative and selective superiority of TSN agar, incubated at 46 C, over SPS agar.Entities:
Keywords: CLASSIFICATION; CLOSTRIDIUM; CLOSTRIDIUM PERFRINGENS; CULTURE MEDIA; EXPERIMENTAL LAB STUDY; NEOMYCIN; PEPTONES; PHARMACOLOGY; PROTEUS; SALMONELLA; STREPTOCOCCUS; SULFITES; TEMPERATURE; TRYPSIN
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Year: 1965 PMID: 14339262 PMCID: PMC1058297 DOI: 10.1128/am.13.4.559-563.1965
Source DB: PubMed Journal: Appl Microbiol ISSN: 0003-6919