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Literature DB >> 424303

Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA.

Abstract

A 1700 nucleotide DNA sequence derived from Xenopus vitellogenin mRNA has been cloned in the bacterial plasmid pBR322. The identity of the cloned sequence was verified in two ways. Firstly, the plasmid DNA was shown to hybridise to an RNA of the correct size (6,700 nucleotides). This was shown by in situ hybridisation to electrophoretically separated RNA and also by the formation of "R-loops" with purified vitellogenin mRNA. Then, using a novel procedure in which plasmid DNA covalently bound to diazotised paper is used to select complementary mRNA sequences, the cloned sequence was shown to hybridise to an mRNA which directed the synthesis of vitellogenin when translated in a reticulocyte lysate cell-free system.

Entities: Gene Species

Mesh：

Substances：

Year: 1979 PMID： 424303 PMCID： PMC327709 DOI： 10.1093/nar/6.2.487

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

25 in total

1. The expression of the vitellogenin gene.

Authors: J R Tata
Journal: Cell Date: 1976-09 Impact factor: 41.582

2. Kinetics of estrogen induction of Xenopus laevis vitellogenin messenger RNA as measured by hybridization to complementary DNA.

Authors: H J Baker; D J Shapiro
Journal: J Biol Chem Date: 1977-12-10 Impact factor: 5.157

3. Purification and characterization of Xenopus laevis vitellogenin messenger RNA.

Authors: D J Shapiro; H J Baker
Journal: J Biol Chem Date: 1977-08-10 Impact factor: 5.157

4. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

5. Quantitative analysis of specific labelled RNA'S using DNA covalently linked to diazobenzyloxymethyl-paper.

Authors: G R Stark; J G Williams
Journal: Nucleic Acids Res Date: 1979-01 Impact factor: 16.971

6. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.

Authors: B M Paterson; B E Roberts; E L Kuff
Journal: Proc Natl Acad Sci U S A Date: 1977-10 Impact factor: 11.205

7. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors: J C Alwine; D J Kemp; G R Stark
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

8. Electron-microscopic demonstration of terminal and internal initiation sites for cDNA synthesis on vitellogenin mRNA.

Authors: W Wahli; T Wyler; R Weber; G U Ryffel
Journal: Eur J Biochem Date: 1978-05

9. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors: F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal: Gene Date: 1977 Impact factor: 3.688

10. R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.

Authors: R L White; D S Hogness
Journal: Cell Date: 1977-02 Impact factor: 41.582

22 in total

1. Cloned DNA sequences complementary to mRNAs encoding precursors to the small subunit of ribulose-1,5-bisphosphate carboxylase and a chlorophyll a/b binding polypeptide.

Authors: R Broglie; G Bellemare; S G Bartlett; N H Chua; A R Cashmore
Journal: Proc Natl Acad Sci U S A Date: 1981-12 Impact factor: 11.205

2. Genetic regulation of the qa gene cluster of Neurospora crassa: induction of qa messenger ribonucleic acid and dependency on qa-1 function.

Authors: W R Reinert; V B Patel; N H Giles
Journal: Mol Cell Biol Date: 1981-09 Impact factor: 4.272

3. Subnuclear fractionation by mild micrococcal-nuclease treatment of nuclei of different transcriptional activities causes a partition of expressed and non-expressed genes.

Authors: G J Dimitriadis; J R Tata
Journal: Biochem J Date: 1980-05-01 Impact factor: 3.857

Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA.

1. The expression of the vitellogenin gene.

2. Kinetics of estrogen induction of Xenopus laevis vitellogenin messenger RNA as measured by hybridization to complementary DNA.

3. Purification and characterization of Xenopus laevis vitellogenin messenger RNA.

4. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

5. Quantitative analysis of specific labelled RNA'S using DNA covalently linked to diazobenzyloxymethyl-paper.

6. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.

7. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

8. Electron-microscopic demonstration of terminal and internal initiation sites for cDNA synthesis on vitellogenin mRNA.

9. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

10. R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.

1. Cloned DNA sequences complementary to mRNAs encoding precursors to the small subunit of ribulose-1,5-bisphosphate carboxylase and a chlorophyll a/b binding polypeptide.

2. Genetic regulation of the qa gene cluster of Neurospora crassa: induction of qa messenger ribonucleic acid and dependency on qa-1 function.

3. Subnuclear fractionation by mild micrococcal-nuclease treatment of nuclei of different transcriptional activities causes a partition of expressed and non-expressed genes.

4. Structural analysis of the three vitellogenin genes in Drosophila melanogaster.

5. Ribosomal RNA genes of Trypanosoma brucei. Cloning of a rRNA gene containing a mobile element.

6. cDNA recombinant plasmid complementary to mRNAs for light chains 1 and 3 of mouse skeletal muscle myosin.

7. Gene amplification in Rhynchosciara salivary gland chromosomes.

8. Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-gamma 2a heavy chain.

9. The preparation and characterization of vitellogenin messenger RNA from rainbow trout (Salmo gairdneri).

10. A gene family in Drosophila melanogaster coding for trypsin-like enzymes.