Literature DB >> 6294613

Ribosomal RNA genes of Trypanosoma brucei. Cloning of a rRNA gene containing a mobile element.

G Hasan, M J Turner, J S Cordingley.   

Abstract

An ordered restriction map of the ribosomal RNA genes of Trypanosoma brucei brucei is presented. Bgl II fragments of T.b.brucei genomic DNA were cloned into pAT 153, and the clones containing rDNA identified. Restriction maps were established and the sense strands identified. One clone was shown by heteroduplex mapping to contain a 1.1 kb inserted sequence which was demonstrated to be widely distributed throughout the genomes of members of the subgenus Trypanozoon. However, in two other subgenera of Trypanosoma, Nannomonas and Schizotrypanum, the sequence is far less abundant. Analysis of the genomic DNA from two serodemes of T.b.brucei showed that the sequence was present in the rRNA of only one of them, implying that the sequence is a mobile element and that its appearance in rDNA is a comparitively recent occurrence.

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Year:  1982        PMID： 6294613      PMCID： PMC326961          DOI： 10.1093/nar/10.21.6747

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  Discontinuity of 26 s rRNA in Acanthamoeba castellani.

Authors:  A R Stevens; P F Pachler
Journal:  J Mol Biol       Date:  1972-05-14       Impact factor: 5.469

3.  Late steps in the maturation of Drosophila 26 S ribosomal RNA: generation of 5-8 S and 2 S RNAs by cleavages occurring in the cytoplasm.

Authors:  B R Jordan; R Jourdan; B Jacq
Journal:  J Mol Biol       Date:  1976-02-15       Impact factor: 5.469

4.  Sequence arrangement of the 16S and 26S rRNA genes in the pathogenic haemoflagellate Leishmania donovani.

Authors:  W Leon; D L Fouts; J Manning
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971

Review 5.  Repeated genes in eukaryotes.

Authors:  E O Long; I B Dawid
Journal:  Annu Rev Biochem       Date:  1980       Impact factor: 23.643

6.  6.5 S RNA; preliminary characterisation of unusual small RNAs in Trypanosoma brucei.

Authors:  J S Cordingley; M J Turner
Journal:  Mol Biochem Parasitol       Date:  1980-04       Impact factor: 1.759

7.  Expression of ribosomal DNA insertions in Drosophila melanogaster.

Authors:  E O Long; I B Dawid
Journal:  Cell       Date:  1979-12       Impact factor: 41.582

8.  Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA.

Authors:  D F Smith; P F Searle; J G Williams
Journal:  Nucleic Acids Res       Date:  1979-02       Impact factor: 16.971

9.  Trans-complementable copy-number mutants of plasmid ColE1.

Authors:  A J Twigg; D Sherratt
Journal:  Nature       Date:  1980-01-10       Impact factor: 49.962

10.  Orphons: dispersed genetic elements derived from tandem repetitive genes of eucaryotes.

Authors:  G Childs; R Maxson; R H Cohn; L Kedes
Journal:  Cell       Date:  1981-03       Impact factor: 41.582
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  10 in total

1.  Analysis of a donor gene region for a variant surface glycoprotein and its expression site in African trypanosomes.

Authors:  D J LaCount; N M El-Sayed; S Kaul; D Wanless; C M Turner; J E Donelson
Journal:  Nucleic Acids Res       Date:  2001-05-15       Impact factor: 16.971

2.  Molecular cloning of an olfactory gene from Drosophila melanogaster.

Authors:  G Hasan
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

3.  The structure and transcription of an element interspersed between tandem arrays of mini-exon donor RNA genes in Trypanosoma brucei.

Authors:  M Carrington; I Roditi; R O Williams
Journal:  Nucleic Acids Res       Date:  1987-12-23       Impact factor: 16.971

4.  Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to domain VII of other eukaryotic 28S rRNAs.

Authors:  T C White; G Rudenko; P Borst
Journal:  Nucleic Acids Res       Date:  1986-12-09       Impact factor: 16.971

5.  Cloning and characterisation of the rRNA genes from the human malaria parasite Plasmodium falciparum.

Authors:  G Langsley; J E Hyde; M Goman; J G Scaife
Journal:  Nucleic Acids Res       Date:  1983-12-20       Impact factor: 16.971

6.  Modification of Trypanosoma brucei mitochondrial rRNA by posttranscriptional 3' polyuridine tail formation.

Authors:  B K Adler; M E Harris; K I Bertrand; S L Hajduk
Journal:  Mol Cell Biol       Date:  1991-12       Impact factor: 4.272

7.  A rapidly rearranging retrotransposon within the miniexon gene locus of Crithidia fasciculata.

Authors:  A Gabriel; T J Yen; D C Schwartz; C L Smith; J D Boeke; B Sollner-Webb; D W Cleveland
Journal:  Mol Cell Biol       Date:  1990-02       Impact factor: 4.272

8.  Evolutionary diversity of eukaryotic small-subunit rRNA genes.

Authors:  M L Sogin; H J Elwood; J H Gunderson
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

9.  Alpha-amanitin-insensitive transcription of variant surface glycoprotein genes provides further evidence for discontinuous transcription in trypanosomes.

Authors:  J M Kooter; P Borst
Journal:  Nucleic Acids Res       Date:  1984-12-21       Impact factor: 16.971

10.  Chromosomes of kinetoplastida.

Authors:  L H Van der Ploeg; A W Cornelissen; J D Barry; P Borst
Journal:  EMBO J       Date:  1984-12-20       Impact factor: 11.598
  10 in total

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