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Literature DB >> 6262726

Structural analysis of the three vitellogenin genes in Drosophila melanogaster.

D C Riddell, M J Higgins, B J McMillan, B N White.

Abstract

Genomic fragments coding for sequences expressed as abundant mRNA in female Drosophila melanogaster were isolated from a lambda library. Hybridization of these clones to polytene chromosomes. in situ, identified four which mapped to X chromosomal region 9A to 9B, the locus for yolk proteins 1 and 2 (Ypl,2) and two which mapped to 12A6-7 to 12D3, the locus for Yp3. These clones were mapped with restriction enzymes, and the coding regions and regions of homology determined by Southern blots probed with cDNA, 5'-end-labelled RNA and nick-translated DNA. Heteroduplex and R-loop mapping confirmed that three of the clones carried two genes separated by about 1.4 kb and oriented in opposite directions. Southern blots probed with cDNA made from alkali-hydrolyzed RNA showed that these genes had their 5' ends next to each other. All 3 genes show homology to each other and have a main coding region of about 1.3 kb, the approximate size for the mRNAs.

Entities: Gene Species

Mesh：

Substances：

Year: 1981 PMID： 6262726 PMCID： PMC326760 DOI： 10.1093/nar/9.6.1323

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

26 in total

1. Nucleic acid hybridization to the DNA of cytological preparations.

Authors: M L Pardue; J G Gall
Journal: Methods Cell Biol Date: 1975 Impact factor: 1.441

2. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

Authors: J M Bailey; N Davidson
Journal: Anal Biochem Date: 1976-01 Impact factor: 3.365

3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

4. Selectivity of yolk protein uptake: comparison of vitellogenins of two insects.

Authors: J G Kunkel; M L Pan
Journal: J Insect Physiol Date: 1976 Impact factor: 2.354

5. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors: W D Benton; R W Davis
Journal: Science Date: 1977-04-08 Impact factor: 47.728

6. Accumulation and degradation of three major yolk proteins in Drosophila melanogaster.

Authors: M Bownes; B D Hames
Journal: J Exp Zool Date: 1977-04

7. A membrane-filter technique for the detection of complementary DNA.

Authors: D T Denhardt
Journal: Biochem Biophys Res Commun Date: 1966-06-13 Impact factor: 3.575

8. Mapping adenines, guanines, and pyrimidines in RNA.

Authors: H Donis-Keller; A M Maxam; W Gilbert
Journal: Nucleic Acids Res Date: 1977-08 Impact factor: 16.971

9. R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.

Authors: R L White; D S Hogness
Journal: Cell Date: 1977-02 Impact factor: 41.582

10. Nucleotide sequence of the rightward operator of phage lambda.

Authors: T Maniatis; A Jeffrey; D G Kleid
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

16 in total

1. Sequence and expression of the Cc gene, a member of the dopa decarboxylase gene cluster of Drosophila: possible translational regulation.

Authors: D D Eveleth; J L Marsh
Journal: Nucleic Acids Res Date: 1986-08-11 Impact factor: 16.971

2. Sex- and cell-specific regulation of yolk polypeptide genes introduced into Drosophila by P-element-mediated gene transfer.

Authors: T Tamura; C Kunert; J Postlethwait
Journal: Proc Natl Acad Sci U S A Date: 1985-10 Impact factor: 11.205

Structural analysis of the three vitellogenin genes in Drosophila melanogaster.

1. Nucleic acid hybridization to the DNA of cytological preparations.

2. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

4. Selectivity of yolk protein uptake: comparison of vitellogenins of two insects.

5. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

6. Accumulation and degradation of three major yolk proteins in Drosophila melanogaster.

7. A membrane-filter technique for the detection of complementary DNA.

8. Mapping adenines, guanines, and pyrimidines in RNA.

9. R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.

10. Nucleotide sequence of the rightward operator of phage lambda.

1. Sequence and expression of the Cc gene, a member of the dopa decarboxylase gene cluster of Drosophila: possible translational regulation.

2. Sex- and cell-specific regulation of yolk polypeptide genes introduced into Drosophila by P-element-mediated gene transfer.

3. Yolk polypeptide gene expression in Minute Drosophila females.

4. An evolutionary model for the insect vitellins.

5. Sequence homologies among the three yolk polypeptide (Yp) genes in Drosophila melanogaster.

6. Yolk polypeptide gene expression in cultured Drosophila cells.

7. Molecular cloning and analysis of the chromosomal region 26A of Drosophila melanogaster.

8. A gene family in Drosophila melanogaster coding for trypsin-like enzymes.

9. Expression of a cystine-rich fish antifreeze in transgenic Drosophila melanogaster.

10. Organization of soybean chalcone synthase gene clusters and characterization of a new member of the family.