Literature DB >> 270678

Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.

B M Paterson, B E Roberts, E L Kuff.   

Abstract

We present a simple method for directly correlating structural gene sequences in DNA with their corresponding mRNAs. This is based upon the fact that mRNA hybridized with its complementary DNA will not direct the cell-free synthesis of a complete polypeptide. Full translational activity of the mRNA is recovered upon the heat melting of the hybrid. Utilizing the rabbit beta globin clone PbetaG1, we demonstrate the application of hybrid-arrested translation for the identification of structural gene sequences within recombinant DNA molecules. In addition, the method is used to locate and order precisely several adenovirus 2 polypeptides within the viral genome.

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Year:  1977        PMID： 270678      PMCID： PMC431943          DOI： 10.1073/pnas.74.10.4370

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

Authors:  J Casey; N Davidson
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

2.  Polypeptides specified by the influenza virus genoma. 2. Assignement of protein coding functions to individual genome segments by in vitro translation.

Authors:  S C Inglis; D J McGeoch; B W Mahy
Journal:  Virology       Date:  1977-05-15       Impact factor: 3.616

3.  RNA-DNA hybridization in solution without DNA reannealing.

Authors:  B Vogelstein; D Gillespie
Journal:  Biochem Biophys Res Commun       Date:  1977-04-25       Impact factor: 3.575

4.  The primary structure of rabbit beta-globin mRNA as determined from cloned DNA.

Authors:  A Efstratiadis; F C Kafatos; T Maniatis
Journal:  Cell       Date:  1977-04       Impact factor: 41.582

5.  An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors:  H R Pelham; R J Jackson
Journal:  Eur J Biochem       Date:  1976-08-01

6.  Hybridization of RNA to double-stranded DNA: formation of R-loops.

Authors:  M Thomas; R L White; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205

7.  Amplification and characterization of a beta-globin gene synthesized in vitro.

Authors:  T Maniatis; S G Kee; A Efstratiadis; F C Kafatos
Journal:  Cell       Date:  1976-06       Impact factor: 41.582

8.  Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures.

Authors:  R C Strohman; P S Moss; J Micou-Eastwood; D Spector; A Przybyla; B Paterson
Journal:  Cell       Date:  1977-02       Impact factor: 41.582

9.  Specific excision of the inserted DNA segment from hybrid plasmids constructed by the poly(dA). poly (dT) method.

Authors:  H Hofstetter; A Schamböck; J Van Den Berg; C Weissmann
Journal:  Biochim Biophys Acta       Date:  1976-12-13

10.  Spliced segments at the 5' terminus of adenovirus 2 late mRNA.

Authors:  S M Berget; C Moore; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205
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  135 in total

1.  Cloned pairs of variable region genes for immunoglobulin heavy chains isolated from a clone library of the entire mouse genome.

Authors:  D J Kemp; S Cory; J M Adams
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  Filter replicas and permanent collections of recombinant DNA plasmids.

Authors:  J P Gergen; R H Stern; P C Wensink
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

3.  Hybridization arrest of cell-free translation of the malarial dihydrofolate reductase/thymidylate synthase mRNA by anti-sense oligodeoxyribonucleotides.

Authors:  C Sartorius; R M Franklin
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971

4.  Isolation and characterization of Borna disease agent cDNA clones.

Authors:  W I Lipkin; G H Travis; K M Carbone; M C Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

5.  Peptide Mapping Reveals Considerable Sequence Homology among the Three Polypeptide Subunits of G1 Storage Protein from French Bean Seed.

Authors:  Y Ma; F A Bliss; T C Hall
Journal:  Plant Physiol       Date:  1980-11       Impact factor: 8.340

Review 6.  Brothers in arms: DNA enzymes, short interfering RNA, and the emerging wave of small-molecule nucleic acid-based gene-silencing strategies.

Authors:  Ravinay Bhindi; Roger G Fahmy; Harry C Lowe; Colin N Chesterman; Crispin R Dass; Murray J Cairns; Edward G Saravolac; Lun-Quan Sun; Levon M Khachigian
Journal:  Am J Pathol       Date:  2007-08-23       Impact factor: 4.307

7.  What history tells us XIV. Regulation of gene expression by non-coding RNAs: the early steps.

Authors:  Michel Morange
Journal:  J Biosci       Date:  2008-09       Impact factor: 1.826

8.  RNA therapeutics: RNAi and antisense mechanisms and clinical applications.

Authors:  Jessica Chery
Journal:  Postdoc J       Date:  2016-07

9.  Calcitonin mRNA polymorphism: peptide switching associated with alternative RNA splicing events.

Authors:  M G Rosenfeld; C R Lin; S G Amara; L Stolarsky; B A Roos; E S Ong; R M Evans
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

10.  Hybridization-selected translation of Bombyx mori high-cysteine chorion proteins in Xenopus laevis oocytes.

Authors:  S C Bock; D C Tiemeier; K Mester; M Wu; M R Goldsmith
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205
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