Literature DB >> 3902895

Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells.

J Burns, V T Chan, J A Jonasson, K A Fleming, S Taylor, J O McGee.   

Abstract

A fast and sensitive method for detecting biotinylated deoxyribonucleic acid (DNA) probes was used for sex determination of cells and tissues by in situ hybridisation of a probe "specific" for the Y chromosome (pHY 2.1). Within 24 hours this procedure visualizes the Y chromosome in fetal and adult cells and tissue, without background noise. This procedure should facilitate antenatal determination of sex on small numbers of uncultured cells. The sensitivity of the procedure also permits the chromosomal assignment of genes present in low copy number.

Mesh：

Substances：

Year:  1985        PMID： 3902895      PMCID： PMC499446          DOI： 10.1136/jcp.38.10.1085

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  9 in total

1.  A rapid screening test for antenatal sex determination.

Authors:  Y F Lau; J C Huang; A M Dozy; Y W Kan
Journal:  Lancet       Date:  1984-01-07       Impact factor: 79.321

2.  DNA in medicine. Oncogenes.

Authors:  R A Weiss; C J Marshall
Journal:  Lancet       Date:  1984-11-17       Impact factor: 79.321

3.  Estimation of aneuploidy levels in human spermatozoa using chromosome specific probes and in situ hybridisation.

Authors:  A M Joseph; J R Gosden; A C Chandley
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

4.  The development capacity of blastomeres from 4- and 8-cell sheep embryos.

Authors:  S M Willadsen
Journal:  J Embryol Exp Morphol       Date:  1981-10

5.  High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry.

Authors:  F Gallyas; T Görcs; I Merchenthaler
Journal:  J Histochem Cytochem       Date:  1982-02       Impact factor: 2.479

6.  Characterisation of a human Y chromosome repeated sequence and related sequences in higher primates.

Authors:  H J Cooke; J Schmidtke; J R Gosden
Journal:  Chromosoma       Date:  1982       Impact factor: 4.316

7.  Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Authors:  P R Langer; A A Waldrop; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

8.  Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.

Authors:  D J Brigati; D Myerson; J J Leary; B Spalholz; S Z Travis; C K Fong; G D Hsiung; D C Ward
Journal:  Virology       Date:  1983-04-15       Impact factor: 3.616

9.  High-resolution mapping of satellite DNA using biotin-labeled DNA probes.

Authors:  L Manuelidis; P R Langer-Safer; D C Ward
Journal:  J Cell Biol       Date:  1982-11       Impact factor: 10.539
  9 in total
  56 in total

1.  Nonradioactive In Situ Hybridization: Recent Techniques and Applications.

Authors:  Masayuki Hara; Shozo Yamada; Kazuaki Hirata
Journal:  Endocr Pathol       Date:  1998       Impact factor: 3.943

2.  In situ hybridisation of EBV DNA-DNA hybrids using wet heat in polypropylene containers.

Authors:  L G Labrecque
Journal:  J Clin Pathol       Date:  1992-12       Impact factor: 3.411

3.  Application of DNA techniques for identification using human dental pulp as a source of DNA.

Authors:  L Pötsch; U Meyer; S Rothschild; P M Schneider; C Rittner
Journal:  Int J Legal Med       Date:  1992       Impact factor: 2.686

Review 4.  Interphase cytogenetics.

Authors:  C S Herrington; J O McGee
Journal:  Neurochem Res       Date:  1990-04       Impact factor: 3.996

Review 5.  In situ hybridisation in perspective.

Authors:  A Warford; I Lauder
Journal:  J Clin Pathol       Date:  1991-03       Impact factor: 3.411

6.  Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy.

Authors:  E Viegas-Pequignot; B Dutrillaux; H Magdelenat; M Coppey-Moisan
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

7.  Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridisation.

Authors:  J Burns; A K Graham; C Frank; K A Fleming; M F Evans; J O McGee
Journal:  J Clin Pathol       Date:  1987-08       Impact factor: 3.411

8.  Epstein-Barr virus genomes in lymphoid cells: activation in mitosis and chromosomal location.

Authors:  C G Teo; B E Griffin
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

9.  A rare heteromorphism of chromosome 20 and reproductive loss.

Authors:  D R Romain; S Whyte; D F Callen; H J Eyre
Journal:  J Med Genet       Date:  1991-07       Impact factor: 6.318

10.  Non-radioactive in situ hybridization. A comparison of several immunocytochemical detection systems using reflection-contrast and electron microscopy.

Authors:  A F Cremers; N Jansen in de Wal; J Wiegant; R W Dirks; P Weisbeek; M van der Ploeg; J E Landegent
Journal:  Histochemistry       Date:  1987
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.