Literature DB >> 3610673

Non-radioactive in situ hybridization. A comparison of several immunocytochemical detection systems using reflection-contrast and electron microscopy.

A F Cremers, N Jansen in de Wal, J Wiegant, R W Dirks, P Weisbeek, M van der Ploeg, J E Landegent.   

Abstract

A number of immunocytochemical detection systems for determining the chromosomal localization of specific nucleic acid sequences by non-radioactive in situ hybridization have been compared. The procedures were: 1. the peroxidase/diaminobenzidine (PO/DAB) combination, either or not gold/silver intensificated; 2. alkaline phosphatase marking using the nitro-blue tetrazolium plus bromochloro-indolyl phosphate substrate combination (AP/NBT + BCIP); and 3. immunogold with or without silver enhancement. The procedures were first tested and optimized in dot blot experiments and then applied to in situ hybridization. As hybridization probes, both a middle-repetitive and a unique sequence (modified with 2-acetylaminofluorene (AAF] were used. The advantages and disadvantages of the various methods for reflection contrast (RC) or transmission electron microscopic (TEM) visualization of hybrids are discussed.

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Year:  1987        PMID： 3610673     DOI： 10.1007/bf00489555

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  37 in total

1.  Sensitive detection of hybridocytochemical results by means of reflection-contrast microscopy.

Authors:  J E Landegent; N Jansen in de Wal; J S Ploem; M Van der Ploeg
Journal:  J Histochem Cytochem       Date:  1985-12       Impact factor: 2.479

2.  A non-radioactive in situ hybridization method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands.

Authors:  A H Hopman; J Wiegant; G I Tesser; P Van Duijn
Journal:  Nucleic Acids Res       Date:  1986-08-26       Impact factor: 16.971

3.  Bi-color detection of two target DNAs by non-radioactive in situ hybridization.

Authors:  A H Hopman; J Wiegant; A K Raap; J E Landegent; M van der Ploeg; P van Duijn
Journal:  Histochemistry       Date:  1986

4.  Formation and detection of RNA-DNA hybrid molecules in cytological preparations.

Authors:  J G Gall; M L Pardue
Journal:  Proc Natl Acad Sci U S A       Date:  1969-06       Impact factor: 11.205

5.  Increased sensitivity in peroxidase immunocytochemistry. A comparative study of a number of peroxidase visualization methods employing a model system.

Authors:  L Scopsi; L I Larsson
Journal:  Histochemistry       Date:  1986

6.  Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells.

Authors:  J Burns; V T Chan; J A Jonasson; K A Fleming; S Taylor; J O McGee
Journal:  J Clin Pathol       Date:  1985-10       Impact factor: 3.411

7.  Immunological method for mapping genes on Drosophila polytene chromosomes.

Authors:  P R Langer-Safer; M Levine; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

8.  Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.

Authors:  D Pinkel; T Straume; J W Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205

9.  High-resolution mapping of satellite DNA using biotin-labeled DNA probes.

Authors:  L Manuelidis; P R Langer-Safer; D C Ward
Journal:  J Cell Biol       Date:  1982-11       Impact factor: 10.539

10.  Mapping muscle protein genes by in situ hybridization using biotin-labeled probes.

Authors:  D G Albertson
Journal:  EMBO J       Date:  1985-10       Impact factor: 11.598
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  10 in total

1.  In situ hybridisation of EBV DNA-DNA hybrids using wet heat in polypropylene containers.

Authors:  L G Labrecque
Journal:  J Clin Pathol       Date:  1992-12       Impact factor: 3.411

Review 2.  In situ hybridization of messenger RNA sequences.

Authors:  L I Larsson
Journal:  Histochem J       Date:  1989-08

3.  Alkaline phosphatase activity in the brain of the American cockroach Periplaneta americana L.

Authors:  P D Verhaert; H R Walgraeve; R G Downer
Journal:  Histochem J       Date:  1990-11

4.  Localization of cytomegalovirus DNA in plastic-embedded sections by in situ hybridization. A methodologic study.

Authors:  M Cao; J H Beckstead
Journal:  Am J Pathol       Date:  1989-02       Impact factor: 4.307

5.  Multicolour preparations for in situ hybridization using precipitating enzyme cytochemistry in combination with reflection contrast microscopy.

Authors:  E J Speel; M Kamps; J Bonnet; F C Ramaekers; A H Hopman
Journal:  Histochemistry       Date:  1993-11

6.  Reflection contrast microscopy. Visualization of (peroxidase-generated) diaminobenzidine polymer products and its underlying optical phenomena.

Authors:  I Cornelese-ten Velde; J Bonnet; H J Tanke; J S Ploem
Journal:  Histochemistry       Date:  1988

7.  Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization.

Authors:  J E Landegent; N Jansen in de Wal; R W Dirks; F Baao; M van der Ploeg
Journal:  Hum Genet       Date:  1987-12       Impact factor: 4.132

8.  Sensitivity of in situ detection with biotinylated probes of human papilloma virus type 16 DNA in frozen tissue sections of squamous cell carcinomas of the cervix.

Authors:  J M Walboomers; W J Melchers; H Mullink; C J Meijer; A Struyk; W G Quint; J van der Noordaa; J ter Schegget
Journal:  Am J Pathol       Date:  1988-06       Impact factor: 4.307

9.  Enzymatic detection systems for non-isotopic in situ hybridization using biotinylated cDNA probes.

Authors:  A Trabandt; R E Gay; V P Sikhatme; S Gay
Journal:  Histochem J       Date:  1995-04

10.  Construction of repeat-free fluorescence in situ hybridization probes.

Authors:  Joost F Swennenhuis; Brad Foulk; Frank A W Coumans; Leon W M M Terstappen
Journal:  Nucleic Acids Res       Date:  2011-11-28       Impact factor: 16.971
  10 in total

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