Literature DB >> 6201432

Estimation of aneuploidy levels in human spermatozoa using chromosome specific probes and in situ hybridisation.

A M Joseph, J R Gosden, A C Chandley.   

Abstract

The paper describes an attempt to estimate the frequency of aneuploid human spermatozoa with disomic Y chromosome and disomic chromosome 1 complements, using chromosome specific probes and in situ hybridisation. This approach was used as an alternative to the differential staining techniques that have been applied to spermatozoa in previous studies aimed at estimating levels of aneuploidy for chromosome 1 and the Y chromosome. A frequency of 1.8 per 1000 YY-bearing spermatozoa and 3.5 per 1000 disomy 1 spermatozoa was found, both figures being in excess of those found by sperm genome karyotyping. The technical limitations of the method are discussed.

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Year:  1984        PMID： 6201432     DOI： 10.1007/bf00286608

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  23 in total

1.  Studies on frequency of Y chromatin in human sperm.

Authors:  E Schwinger; J Ites; B Korte
Journal:  Hum Genet       Date:  1976-12-15       Impact factor: 4.132

2.  The chromosomal distribution of human satellite III DNA during meiosis.

Authors:  H Seuanez; A R Mitchell; J R Gosden
Journal:  Cytobios       Date:  1976

3.  Specific staining of the human No. 1 chromosome in spermatozoa.

Authors:  J Geraedts; P Pearson
Journal:  Humangenetik       Date:  1973

Review 4.  The use of new staining techniques for human chromosome identification.

Authors:  P Pearson
Journal:  J Med Genet       Date:  1972-09       Impact factor: 6.318

5.  Chromosomal aneuploidy in human spermatozoa.

Authors:  I H Pawlowitzki; P L Pearson
Journal:  Humangenetik       Date:  1972

6.  A cloned repeated DNA sequence in human chromosome heteromorphisms.

Authors:  J R Gosden; S S Lawrie; H J Cooke
Journal:  Cytogenet Cell Genet       Date:  1981

7.  F-bodies as Y chromosome markers in mature human sperm heads: a quantitative approach.

Authors:  R A Beatty
Journal:  Cytogenet Cell Genet       Date:  1977

8.  The location of four human satellite DNAs on human chromosomes.

Authors:  J R Gosden; A R Mitchell; R A Buckland; R P Clayton; H J Evans
Journal:  Exp Cell Res       Date:  1975-04       Impact factor: 3.905

9.  Characterisation of a human Y chromosome repeated sequence and related sequences in higher primates.

Authors:  H J Cooke; J Schmidtke; J R Gosden
Journal:  Chromosoma       Date:  1982       Impact factor: 4.316

Review 10.  High-resolution chromosome analysis in clinical medicine.

Authors:  J J Yunis; M E Chandler
Journal:  Prog Clin Pathol       Date:  1978
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  19 in total

1.  Non-isotopic detection of chromosome 1 in human meiosis and demonstration of disomic sperm nuclei.

Authors:  M Guttenbach; M Schmid
Journal:  Hum Genet       Date:  1991-07       Impact factor: 4.132

2.  Determination of Y chromosome aneuploidy in human sperm nuclei by nonradioactive in situ hybridization.

Authors:  M Guttenbach; M Schmid
Journal:  Am J Hum Genet       Date:  1990-03       Impact factor: 11.025

3.  Efficacy of calcium ionophore A23187 oocyte activation for generating parthenotes for human embryo research.

Authors:  A Rhoton-Vlasak; P Y Lu; K M Barud; G W Dewald; D G Hammitt
Journal:  J Assist Reprod Genet       Date:  1996-11       Impact factor: 3.412

4.  A cloned sequence, p82H, of the alphoid repeated DNA family found at the centromeres of all human chromosomes.

Authors:  A R Mitchell; J R Gosden; D A Miller
Journal:  Chromosoma       Date:  1985       Impact factor: 4.316

Review 5.  The genetics of human reproduction.

Authors:  A C Chandley
Journal:  Experientia       Date:  1986-10-15

6.  Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells.

Authors:  J Burns; V T Chan; J A Jonasson; K A Fleming; S Taylor; J O McGee
Journal:  J Clin Pathol       Date:  1985-10       Impact factor: 3.411

7.  Detection of nondisjunction and recombination in meiotic and postmeiotic cells from XYSxr [XY,Tp(Y)1Ct] mice using multicolor fluorescence in situ hybridization.

Authors:  T Ashley; T Ried; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-18       Impact factor: 11.205

8.  Incidence of chromosome 3, 7, 10, 11, 17 and X disomy in mature human sperm nuclei as determined by nonradioactive in situ hybridization.

Authors:  M Guttenbach; R Schakowski; M Schmid
Journal:  Hum Genet       Date:  1994-01       Impact factor: 4.132

9.  Numerical chromosome abnormalities in spermatozoa of fertile and infertile men detected by fluorescence in situ hybridization.

Authors:  N Miharu; R G Best; S R Young
Journal:  Hum Genet       Date:  1994-05       Impact factor: 4.132

10.  Detection of aneuploid human sperm by fluorescence in situ hybridization: evidence for a donor difference in frequency of sperm disomic for chromosomes 1 and Y.

Authors:  W A Robbins; R Segraves; D Pinkel; A J Wyrobek
Journal:  Am J Hum Genet       Date:  1993-04       Impact factor: 11.025
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