Literature DB >> 6754749

High-resolution mapping of satellite DNA using biotin-labeled DNA probes.

L Manuelidis, P R Langer-Safer, D C Ward.   

Abstract

We have developed a novel method for high resolution mapping of specific DNA sequences after in situ hybridization. DNA probes, labeled with biotin-nucleotides in conventional nick-translation reactions, are hybridized to cytological preparations and detected with affinity-purified rabbit antibiotin antibodies followed by antibodies to rabbit IgG that are conjugated to fluorescent or enzymatic reagents. Using peroxidase labeled anti-rabbit IgG, we are able to detect and localize specific sequences at both the light and electron microscopic levels. Initial studies were done with repeated DNA sequences previously mapped by light microscope autoradiography to assess the fidelity and resolution of this method. An analysis using biotin-labeled mouse satellite DNA is presented here.

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Year:  1982        PMID： 6754749      PMCID： PMC2112973          DOI： 10.1083/jcb.95.2.619

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  22 in total

1.  Mouse satellite DMA in noncentromeric heterochromatin of cultured cells.

Authors:  R White; L M Pasztor; F Hu
Journal:  Chromosoma       Date:  1975       Impact factor: 4.316

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Arrangement of centromeres in mouse cells.

Authors:  T C Hsu; J E Cooper; M L Mace; B R Brinkley
Journal:  Chromosoma       Date:  1971       Impact factor: 4.316

4.  Formation and detection of RNA-DNA hybrid molecules in cytological preparations.

Authors:  J G Gall; M L Pardue
Journal:  Proc Natl Acad Sci U S A       Date:  1969-06       Impact factor: 11.205

5.  The rationale for an ordered arrangement of chromatin in the interphase nucleus.

Authors:  D E Comings
Journal:  Am J Hum Genet       Date:  1968-09       Impact factor: 11.025

6.  Chromosomal and nuclear location of mouse satellite DNA in individual cells.

Authors:  K W Jones
Journal:  Nature       Date:  1970-03-07       Impact factor: 49.962

7.  RNA-DNA hybrids at the cytological level.

Authors:  H A John; M L Birnstiel; K W Jones
Journal:  Nature       Date:  1969-08-09       Impact factor: 49.962

8.  Localization of mouse satellite DNA on chromosomes of experimentally induced glioblastomas; non-centromeric lable in one glioblastoma producing C-type particles.

Authors:  L Manuelidis; E E Manuelidis
Journal:  Int J Cancer       Date:  1976-05-15       Impact factor: 7.396

9.  Amount of satellite DNA in four experimentally induced tumors of the central nervous system. Quantitative changes in a glioblastoma producing C-type particles.

Authors:  L Manuelidis; E E Manuelidis
Journal:  J Natl Cancer Inst       Date:  1976-01       Impact factor: 13.506

10.  Localization of 5-methylcytosine in human metaphase chromosomes by immunoelectron microscopy.

Authors:  B W Lubit; T D Pham; O J Miller; B F Erlanger
Journal:  Cell       Date:  1976-12       Impact factor: 41.582
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  42 in total

Review 1.  Imaging of single DNA molecule: applications to high-resolution genomic studies.

Authors:  J Herrick; A Bensimon
Journal:  Chromosome Res       Date:  1999       Impact factor: 5.239

2.  In situ hybridisation of EBV DNA-DNA hybrids using wet heat in polypropylene containers.

Authors:  L G Labrecque
Journal:  J Clin Pathol       Date:  1992-12       Impact factor: 3.411

3.  A survey of the first hundred volumes of chromosoma.

Authors:  H G Callan
Journal:  Chromosoma       Date:  1992-08       Impact factor: 4.316

4.  A quick and simple FISH protocol with hybridization-sensitive fluorescent linear oligodeoxynucleotide probes.

Authors:  Dan Ohtan Wang; Hitomi Matsuno; Shuji Ikeda; Akiko Nakamura; Hiroyuki Yanagisawa; Yasunori Hayashi; Akimitsu Okamoto
Journal:  RNA       Date:  2011-11-18       Impact factor: 4.942

5.  Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy.

Authors:  E Viegas-Pequignot; B Dutrillaux; H Magdelenat; M Coppey-Moisan
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

6.  Detection of mRNA and hnRNA using a digoxigenin labelled cDNA probe by in situ hybridization on frozen tissue sections.

Authors:  N Maggiano; L M Larocca; M Piantelli; F O Ranelletti; L Lauriola; R Ricci; A Capelli
Journal:  Histochem J       Date:  1991-02

7.  In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using antisense RNA probes.

Authors:  H Hoefler; H Childers; M R Montminy; R M Lechan; R H Goodman; H J Wolfe
Journal:  Histochem J       Date:  1986 Nov-Dec

8.  Microscopy and image analysis.

Authors:  George McNamara; Michael J Difilippantonio; Thomas Ried
Journal:  Curr Protoc Hum Genet       Date:  2005-08

9.  Simultaneous visualization of chromosome bands and hybridization signal using colloidal-gold labeling in electron microscopy.

Authors:  R Fetni; R Drouin; N Lemieux; P E Messier; C L Richer
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-01       Impact factor: 11.205

10.  Non-radioactive in situ hybridization. A comparison of several immunocytochemical detection systems using reflection-contrast and electron microscopy.

Authors:  A F Cremers; N Jansen in de Wal; J Wiegant; R W Dirks; P Weisbeek; M van der Ploeg; J E Landegent
Journal:  Histochemistry       Date:  1987
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