Literature DB >> 2415575

Sensitive detection of hybridocytochemical results by means of reflection-contrast microscopy.

J E Landegent, N Jansen in de Wal, J S Ploem, M Van der Ploeg.   

Abstract

A new sensitive method for visualization of nonautoradiographic hybridization results in microscopic preparations is described. The method is based on the reflection of the incident light by diaminobenzidine precipitates deposited at the site of hybridization during an indirect hybridocytochemical procedure. The reflected light is detected by means of reflection-contrast microscopy. The applicability of the procedure is demonstrated with nucleic acid probes modified with 2-acetylaminofluorene groups. These in turn are localized in situ by an indirect immunoperoxidase reaction. Besides its sensitivity, this simple visualization technique possesses the additional advantages, over absorption and fluorescence microscopy, that it provides a total DNA counterstain and a chromosomal banding pattern.

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Year:  1985        PMID： 2415575     DOI： 10.1177/33.12.2415575

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  25 in total

1.  Comparison of different detection methods in quantitative microdensitometry.

Authors:  L Ermert; A C Hocke; H R Duncker; W Seeger; M Ermert
Journal:  Am J Pathol       Date:  2001-02       Impact factor: 4.307

2.  Meiotic chromosome behaviour reflects levels of sequence divergence in Sus scrofa domestica satellite DNA.

Authors:  M Jantsch; B Hamilton; B Mayr; D Schweizer
Journal:  Chromosoma       Date:  1990-09       Impact factor: 4.316

3.  Chromosomal insertion of human papillomavirus 18 sequences in HeLa cells detected by nonisotopic in situ hybridization and reflection contrast microscopy.

Authors:  P F Ambros; H I Karlic
Journal:  Hum Genet       Date:  1987-11       Impact factor: 4.132

4.  Putting the genetics back into cytogenetics.

Authors:  M A Ferguson-Smith
Journal:  Am J Hum Genet       Date:  1991-02       Impact factor: 11.025

5.  Reflection contrast microscopy within chrome-alum haematoxylin stained thick tissue-sections.

Authors:  T J Filler; C H Rickert; U K Fassnacht; F Pera
Journal:  Histochemistry       Date:  1994-06

6.  Quantitative study of enzyme immunocytochemical reactions performed with enzyme conjugates immobilized on nitrocellulose.

Authors:  P H Nibbering; J G Marijnen; A K Raap; P C Leijh; R van Furth
Journal:  Histochemistry       Date:  1986

7.  A new hybridocytochemical method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands. I. Stability of the mercury-sulfhydryl bond and influence of the ligand structure on immunochemical detection of the hapten.

Authors:  A H Hopman; J Wiegant; P van Duijn
Journal:  Histochemistry       Date:  1986

8.  A new hybridocytochemical method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands. II. Effects of variations in ligand structure on the in situ detection of mercurated probes.

Authors:  A H Hopman; J Wiegant; P van Duijn
Journal:  Histochemistry       Date:  1986

9.  Evidence for distinguishable transcripts of the putative testis determining gene (ZFY) and mapping of homologous cDNA sequences to chromosomes X,Y and 9.

Authors:  N A Affara; D Chambers; J O'Brien; S S Habeebu; M Kalaitsidaki; C E Bishop; M A Ferguson-Smith
Journal:  Nucleic Acids Res       Date:  1989-04-25       Impact factor: 16.971

10.  Non-radioactive in situ hybridization. A comparison of several immunocytochemical detection systems using reflection-contrast and electron microscopy.

Authors:  A F Cremers; N Jansen in de Wal; J Wiegant; R W Dirks; P Weisbeek; M van der Ploeg; J E Landegent
Journal:  Histochemistry       Date:  1987
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