Transamidation-Driven Molecular Pumps.

We report a new class of synthetic molecular pumps that use a stepwise information ratchet mechanism to achieve the kinetic gating required to sequester their macrocyclic substrates from bulk solution. Threading occurs as a result of active template reactions between the pump terminus amine and an acyl electrophile, whereby the bond-forming reaction is accelerated through the cavity of a crown ether. Carboxylation of the resulting amide results in displacement of the ring to the collection region of the thread. Conversion of the carbamate to a phenolic ester provides an intermediate rotaxane suitable for further pumping cycles. In this way rings can be ratcheted onto a thread from one or both ends of appropriately designed molecular pumps. Each pumping cycle results in one additional ring being added to the thread per terminus acyl group. The absence of pseudorotaxane states ensures that no dethreading of intermediates occurs during the pump operation. This facilitates the loading of different macrocycles in any chosen sequence, illustrated by the pump-mediated synthesis of a [4]rotaxane containing three different macrocycles as a single sequence isomer. A [5]rotaxane synthesized using a dual-opening transamidation pump was structurally characterized by single-crystal X-ray diffraction, revealing a series of stabilizing CH···O interactions between the crown ethers and the polyethylene glycol catchment region of the thread.

Introduction

Protein pumps actively transport substrates away from equilibrium.[1−4] These biomolecular machines are generally extremely structurally complex, assembled from multiple protein subunits and having molecular masses in excess of 500 kDa. A number of much smaller artificial molecular pumps have been designed.[5−24] These minimalist systems can provide insights into the basic mechanisms required to drive chemical systems away from equilibrium[25,26] and also illustrate well how different structural modules can be combined to generate function that goes far beyond that of the sum of the individual parts.[7,27]

Synthetic molecular pumps based on pseudorotaxane architectures have been used to drive systems away from equilibrium by progressively sequestering macrocycles from bulk solution to thermodynamically less favorable sites on collection threads.[12−21] Accordingly, the macrocycles are trapped in a high energy state on the axle compared to unthreaded rings in solution. This constitutes active transport of the rings from bulk solution to the collection thread.[20,21] Accordingly, the pumping needs to be powered and to occur under kinetic control. The chemical structure of the pump is designed to promote macrocycle threading and inhibit dethreading. Each pumping cycle builds on the last by increasing the concentration of macrocycles held on the collection thread. In this way, molecular pumping also enables the synthesis of well-defined higher order oligo- and polyrotaxanes and catenanes that would be inaccessible through conventional “passive” template synthesis.[13,18,28−32]

Most of the rotaxane-based pumps reported to date employ energy ratchet[5] mechanisms, which rely on periodic variations in the binding affinities and kinetic barriers between the macrocycle and various sites on the pump. The different conditions that occur over the operation cycle define the energy surface accessible to the macrocycle, inhibiting dethreading and driving the ring onto the collection thread. A range of stimuli have been employed to drive such systems, including transition metal coordination,[29,30] acid/base cycling,[13,21,31] radical pairing,[12,14−18,20] and photoisomerizations.[22−24,33,34] Pumping by information ratchet mechanisms[35−38] has also been demonstrated with artificial molecular pumps.[19] Such systems rely on kinetic asymmetry,[36−39] arising from transition state energy differences that depend on the mechanical state of the pump. Information ratchets can operate autonomously in a chemostated environment[40] and likely form the mechanism for most or all biomolecular pumps.[36]

Here we report a new type of synthetic information ratchet pump, 1, which operates through iterative transamidation. Pump 1 operates in a stepwise manner with no dethreadable intermediates, enabling sequence-controlled pumping of different macrocycles onto collection threads.

Results and Discussion

Design and Operation of Single-Opening Transamidation Pump 1

Pump 1, with a single opening for ring-threading, was synthesized as outlined in the Supporting Information (Scheme S1). Its mechanism exploits metal-free active template rotaxane synthesis,[41−44] in which the transition state of a thread-forming reaction between a primary amine and an electrophile is stabilized through the cavity of a crown ether. This results in kinetically controlled trapping of the threaded components.[19,41−45] We chose to focus on N-acylation for the active template reaction, as this had previously been found[43] to be particularly selective toward rotaxane formation over the background reaction that generates the non-interlocked thread. Treatment of 1 with 3,5-bis-trifluoromethylbenzylamine and 24-crown-8 2 for 16 h in toluene afforded [2]rotaxane 3 in 65% yield (Scheme , step i). The threaded structure of 3 was confirmed by 1H NMR, where characteristic diastereotopic splitting of the protons on the different faces of the macrocycle (Ha, see Scheme for proton labeling) results from threading onto an unsymmetric axle (Figure b). Downfield shifts of the benzylic and aromatic protons (Hd and He, from 4.74 to 4.91 ppm and 7.79 to 8.69 ppm, respectively) in 3 compared to those in the non-interlocked thread, 7, indicate that the macrocycle is sited over the amide in the [2]rotaxane.

Scheme 1

Operation of Single-Opening Transamidation Molecular Pump 1

Reagents and conditions: (i) 3,5-bis-trifluoromethylbenzylamine (1.0 equiv), 2 (1.0 equiv), toluene, rt, 16 h, 65%; (ii) Boc2O (6.0 equiv), DMAP (0.2 equiv), THF, 90 °C, 10 h, microwave irradiation, 77%; (iii) 4-bromo-3,5-dimethylphenol (1.0 equiv), K3PO4 (1.5 equiv), THF, 60 °C, 16 h, microwave irradiation, 68%; (iv) 3,5-bis-trifluoromethylbenzylamine (2.0 equiv), 2 (2.0 equiv), toluene, rt, 10 days, 50%.

Figure 1

Partial 1H NMR spectra (600 Hz, 298 K, CDCl3) of the pumping cycle of 1: (a) non-interlocked thread 7; (b) amide [2]rotaxane 3; (c) Boc-activated [2]rotaxane 4; (d) ester [2]rotaxane 5; (e) amide [3]rotaxane 6. For proton labeling, see Scheme .

We envisaged that converting the amide in [2]rotaxane 3 to a reactive electrophile would allow further macrocycles to be pumped onto the thread via transamidation.[46,47] We were inspired by recent methodology reported by Szostak and co-workers,[48,49] in which N-carboxylated amides were shown to undergo transamidation reactions. We reasoned that derivatizing the amide of 3 should also remove its ability to donate hydrogen bonds and thus weaken intercomponent binding and promote shuttling of the macrocycle to the oligo(ethylene glycol) region of the collection thread. Reaction of 3 with di-tert-butyl decarbonate (Boc2O) (see Supporting Information, Table S1, for optimization studies on the amide activation step) gave [2]rotaxane 4 in 77% yield (Scheme , step ii).

Shuttling of the macrocycle to the collection thread upon conversion of 3 to 4 was confirmed by 1H NMR (Figure c). Signals for Ha shifted downfield from 3.42 to 3.60 and 3.18 to 3.55 ppm, together with more modest shifts to the other thread protons proximal to the amide (Hb, Hc, Hd, He, and Hf). The chemical shifts of He and Hf in 4 are similar to those in non-interlocked thread 7 (Figure a), consistent with the displacement of the macrocycle away from the amide.

However, no reaction occurred when [2]rotaxane 4 was subsequently treated with 3,5-bis-trifluoromethylbenzylamine and crown ether 2 in toluene. The Boc-amide was not sufficiently electrophilic and/or too sterically hindered to bring about [3]rotaxane formation in the nonpolar solvents required for the active template reaction. To overcome this issue, we reasoned that a nucleophilic bulky phenol might be able to generate a more electrophilic rotaxane intermediate containing a phenolic ester.[42−45] Active template aminolysis of this ester would then give the [3]rotaxane and regenerate the phenol.

Reaction of [2]rotaxane 4 with 4-bromo-3,5-dimethylphenol and potassium phosphate in THF (for reaction optimization see Table S2, Supporting Information) smoothly generated ester [2]rotaxane 5 in 68% yield (Scheme , step iii). The chemical shifts of macrocyclic protons Ha in 5 are almost unchanged from 4, indicating that the macrocycle remains located on the glycol region of the collection thread.

Pleasingly, the phenolic ester [2]rotaxane 5 enabled [3]rotaxane formation as envisaged: treatment of 5 with 3,5-bis-trifluoromethylbenzylamine and 24-crown-8 2 resulted in [3]rotaxane 6 in 50% yield (Scheme , step iv) to complete a second pumping cycle. The 1H NMR spectrum of [3]rotaxane 6 (Figure e) shows two sets of macrocyclic signals, one set at chemical shifts similar to those in 3 (Figure b) and the other similar to those in 4 (Figure c) and 5 (Figure d). This is consistent with one macrocycle in 6 residing on the collection chain, while the other binds to the newly formed amide.

Synthesis of a Single-Sequence [4]Rotaxane (13) Using a Single-Opening Transamidation Molecular Pump

In principle, the pumping cycle shown in Scheme , steps ii–iv, can be repeated over and over again, pumping on additional rings (one per cycle) until the catchment region of the thread is full. A distinctive feature of the mechanism is that at no point in the pumping cycle are captured macrocycles able to dethread, as the intermediate pump states are all rotaxanes (dethreading is prevented by bulky stoppers on both ends of the axle), rather than pseudorotaxanes, where dethreading is only slowed by “speed bumps”. This should enable the pump to be used to synthesize oligo- or polyrotaxanes with a single sequence of structurally distinct macrocycles pumped in a specific order.[21,29,30,50]

We demonstrated this by synthesizing [4]rotaxane 13 (Supporting Information, Scheme S2), which contains three different 24-crown-8 derivatives threaded in a single sequence and mechanically maintained in that order, on the thread (Scheme ). Nitrophenol ester pump 8 was subjected to three pumping cycles, first using dibenzo-24-crown-8 9 as the macrocycle to give [2]rotaxane 10 (see Supporting Information for synthesis of 13 and intermediates). A pumping cycle on [2]rotaxane 10 with 24-crown-8 (2) as the macrocycle then generated [3]rotaxane 11, and then a third with benzo-24-crown-8 (12) afforded [4]rotaxane 13. Rotaxane 13 was characterized by high-resolution electrospray mass spectrometry (Scheme ) and 1H and 13C NMR spectroscopy (Supporting Information, Spectra S47 and S48). [4]Rotaxane 13 was isolated in 2% overall yield (three pumping cycles; an average of 60% per synthetic step) as the only isomer detected out of six possible arrangement of three different macrocycles.

Scheme 2

Synthesis of Single-Sequence [4]Rotaxane 13

Reagents and conditions: (i) 3,5-bis-trifluoromethylbenzylamine (1.5 equiv), 9 (1.5 equiv), toluene, rt, 16 h, 61% ([2]rotaxane:free thread ratio 5:1, determined by 1H NMR, in the reaction mixture prior to workup); (ii) Boc2O (6.0 equiv), DMAP (1.2 equiv), THF, 90 °C, 10 h, microwave irradiation, 81%; (iii) 4-bromo-3,5-dimethylphenol (3.0 equiv), K3PO4 (4.5 equiv), THF, 70 °C, 8 h, microwave irradiation, 90%; (iv) 3,5-bis-trifluoromethylbenzylamine (2.0 equiv), 2 (2.0 equiv), toluene, rt, 7 days, 54%. (v) Boc2O (6.0 equiv), DMAP (1.2 equiv), THF, 80 °C, 4 h, microwave irradiation, 75%; (vi) 4-bromo-3,5-dimethylphenol (3.0 equiv), K3PO4 (4.5 equiv), THF, 60 °C, 16 h, microwave irradiation, 54%; (vii) 3,5-bis-trifluoromethylbenzylamine (2.0 equiv), 12 (2.0 equiv), toluene, rt, 21 days, 20% (also isolated [3]rotaxane 11, 10%).

Synthesis of [5]Rotaxane 16 with Dual-Opening Transamidation Molecular Pump 14

As the “active” end of the thread features a bulky group that inherently prevents dethreading, the transamidation pumping strategy is particularly well suited for operating with pumping motifs at both ends of a thread. We prepared pump 14, with active esters at either terminus of the catchment region. The design means pump 14 is capable of pumping two macrocycles per transamidation cycle. A bulkier 3,5-dimethyl-4-nitrophenol leaving group was used in 14 to ensure dethreading did not occur en route to [3]rotaxane formation (unsubstituted 4-nitrophenol, the leaving group in 1 and 8, is not sufficiently bulky to prevent dethreading of 2). A single pumping cycle on 14 resulted in [3]rotaxane 15 in 60% yield (Scheme , step i); a second pumping cycle (Scheme , steps ii–iv) gave [5]rotaxane 16 in 9% overall yield from 14.

Scheme 3

Synthesis of [5]Rotaxane 16 Using a Dual-Opening Molecular Pump

Reagents and conditions: (i) 3,5-bis-trifluoromethylbenzylamine (1.0 equiv), 2 (1.0 equiv), toluene, 50 °C, 16 h, 60%; (ii) Boc2O (12.0 equiv), DMAP (0.4 equiv), THF, 80 °C, 10 h, microwave irradiation, 80%; (iii) 4-bromo-3,5-dimethylphenol (3.0 equiv), K3PO4 (4.5 equiv), THF, 60 °C, 16 h, microwave irradiation, 53%; (iv) 3,5-bis-trifluoromethylbenzylamine (2.8 equiv), 2 (5.5 equiv), toluene, rt, 21 days, 35%.

[5]Rotaxane 16 was characterized by high-resolution electrospray ionization spectrometry (Scheme ) and 1H and 13C NMR spectroscopy (Supporting Information, Spectra S61 and S62). Single crystals of 16 suitable for X-ray diffraction were obtained from slow evaporation of a diethyl ether/hexane solution of the rotaxane. The X-ray crystal structure of 16 is shown in Figure .

Figure 2

(a) X-ray crystal structure of [5]rotaxane 16. (b) Expanded view of two macrocycles bound to the amide and on the polyethylene glycol region of the thread, showing hydrogen bond intercomponent CH···O interactions. Hydrogen bond lengths: O4C···HN56, 2.40 Å; O22C···HC57, 2.67 Å; O13F···HC81, 2.51 Å; O80···HC24F, 2.71 Å; O80···HC5F, 2.58 Å; O80···HC6F, 1.99 Å; O83···HC20F, 2.79 Å. Hydrogen bond angles: O4C···H–N56, 151.9°; O22C···H–C57, 148.6°; O80F···H–C24F, 154.8°; O80···H–C5F, 139.3°; O80···H–C6F, 104.9°; O13F···H–C81, 162.3°. (c) View showing CH···O hydrogen bonding of macrocycle on the polyethylene glycol region of the thread. Hydrogen bond lengths: O1B···HC91, 2.56 Å; O7B···HC90, 2.60 Å; O86···HC20B, 2.87 Å; O89···HC23B, 2.57 Å; O92···HC12B, 2.51 Å. Hydrogen bond angles: O1B···H–C91, 117.7°; O7B···H–C90, 114.3°; O86···H–C20B, 161.9°; O89···H–C23B, 152.8°; O92···H–C12B, 131.1°. Carbon, gray; oxygen, red; hydrogen, white; nitrogen, blue; fluorine, yellow. Hydrogen bonds shown in light green. Additional hydrogen atoms and solvent molecules are omitted for clarity.

Despite extensive research on crown ethers over the last 50 years,[51] solid state characterization of complexes between crown ethers and linear oligo(ethylene glycol) chains remains rare.[52] This is likely a reflection of the lack of driving force for such associations and, perhaps, the tendency of such complexes not to form well-defined single crystals. However, the synthesis of [5]rotaxane 16 does not depend on the thermodynamically favored assembly of a host–guest complex, but rather the crown ethers are driven onto the thread by the information ratchet mechanism and kinetically trapped in the out-of-equilibrium state. The X-ray crystal structure of 16 reveals the weak favorable interactions that the components adopt to achieve a relatively low energy coconformation given their forced association.[53]

The solid state structure of 16 is reminiscent of the coconformation NMR indicates is adopted in CDCl3 solution: the two outer macrocycles each bind to a thread amide group through NH···O hydrogen bonding of the amide hydrogen to the crown ether and CH···O=C hydrogen bonding from the crown ether to the amide carbonyl.[28,43,44] The internal macrocycles do not interact with each other; the system is better stabilized by each forming an extensive array of CH···O interactions with the polyethylene glycol thread, including somewhat unexpectedly the relatively electron poor phenolic oxygens.[54,55]

The Effectiveness of the Transamidation Pumping Mechanism

The selectivity of crown-ether-stabilized N-acylation toward threading over non-interlocked axle formation in [2]rotaxane synthesis (i.e., active template synthesis) was previously found to be >100:1 using 24-crown-8 and nitrophenol ester electrophiles.[43] In the case of single-opening pumping of 1 to 3 (Scheme , step i) or dual-opening pumping of 14 to 15 (Scheme , step i), the high selectivity appears to be maintained, and we were not able to isolate any non-interlocked thread (nor [2]rotaxane in the case of Scheme , step i) from the crude reaction mixtures. In the pumping to form 6 (Scheme , step iv), 11 (Scheme , step iv), and 16 (Scheme , step iv), when the electrophile is a 4-bromo-3,5-dimethylphenol ester, the active template transamidation is also highly selective with no signals of [2]rotaxane 3, 10, or [3]rotaxane 15 observed in the 1H NMR of the crude reaction mixtures. The pumping yields are limited by the reactivity of the ester intermediates (5, S11, and S17). In pumping to form [5]rotaxane 16 (Scheme , step iv), the potential [4]rotaxane side-product containing two amides (i.e., a product where both esters have reacted but only one macrocycle has threaded) is not observed. In the active template synthesis of 10 from 8 (Scheme , step i), where dibenzo-24-crown-8 is the macrocycle rather than 24-crown-8, the selectivity toward [2]rotaxane formation over free thread falls to ∼5:1 (determined by 1H NMR of the crude reaction mixture). In the final pumping step to form [4]rotaxane 13, which uses benzo-24-crown-8 as the macrocycle, the selectivity toward threading decreases further: [3]rotaxane 11 was isolated in 10% yield alongside the [4]rotaxane product (20%). Steric congestion from the rings already trapped on the thread likely contributes to the lower selectivity of threading observed in this pumping step.

Conclusions

The combination of transamidation active template synthesis and the activation of amides by carboxylation forms a simple and effective stepwise information ratchet mechanism for iteratively pumping multiple crown ethers from bulk solution onto a collection thread. Phenolic esters provide stable rotaxane intermediates in the pumping cycle. Pumps with a single transamidation module sequester one crown ether from bulk solution onto the collection thread per cycle; molecules with transamidation modules at both ends of the thread add two crown ethers per cycle. Pumping does not require the formation of thermodynamically favorable host–guest complexes on regions of the thread nor macrocycle binding sites in the collection region. The X-ray crystal structure of a [5]rotaxane, synthesized using a dual-opening molecular pump, reveals a coconformation stabilized by arrays of weak CH···O interactions. The stepwise operation of transamidation pumps makes it straightforward to synthesize monodispersed oligorotaxanes with a specific number and sequence of different macrocycles. Until recently, the synthesis of rotaxanes required one thread binding site per macrocycle and sequence isomerism in rotaxanes was virtually unknown.[56] The ability to drive molecular systems directionally away from equilibrium with ratchet mechanisms has ramifications not only for synthesis but for many other aspects of molecular nanotechnology.[7,27,56,57]