Literature DB >> 3562241

Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus.

J N Kremsky, J L Wooters, J P Dougherty, R E Meyers, M Collins, E L Brown.   

Abstract

A general method for the immobilization of DNA through its 5'-end has been developed. A synthetic oligonucleotide, modified at its 5'-end with an aldehyde or carboxylic acid, was attached to latex microspheres containing hydrazide residues. Using T4 polynucleotide ligase and an oligonucleotide splint, a single stranded 98mer was efficiently joined to the immobilized synthetic fragment. After impregnation of the latex microspheres with the fluorescent dye, Nile Red and attachment of an aldehyde 16mer, 5 X 10(5) bead-DNA conjugates could be detected with a conventional fluorimeter.

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Year:  1987        PMID： 3562241      PMCID： PMC340705          DOI： 10.1093/nar/15.7.2891

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  26 in total

1.  RNA hybridization to DNA coupled with cyanogen-bromide-activated sephadex. The purification of polyoma messenger RNA.

Authors:  S G Siddell
Journal:  Eur J Biochem       Date:  1978-12

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Nucleic acid hybridization using DNA covalently coupled to cellulose.

Authors:  B E Noyes; G R Stark
Journal:  Cell       Date:  1975-07       Impact factor: 41.582

4.  Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides.

Authors:  S Agrawal; C Christodoulou; M J Gait
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

5.  Polynucleotide celluloses as solid state primers and templates for polymerases.

Authors:  T M Jovin; A Kornberg
Journal:  J Biol Chem       Date:  1968-01-25       Impact factor: 5.157

6.  The synthesis of celluloses containing covalently bound nucleotides, polynucleotides, and nucleic acids.

Authors:  P T Gilham
Journal:  Biochemistry       Date:  1968-08       Impact factor: 3.162

7.  Covalent attachment of DNA to agarose. Improved synthesis and use in affinity chromatography.

Authors:  D J Arndt-Jovin; T M Jovin; W Bähr; A M Frischauf; M Marquardt
Journal:  Eur J Biochem       Date:  1975-06

8.  High-pressure liquid chromatography in polynucleotide synthesis.

Authors:  H J Fritz; R Belagaje; E L Brown; R H Fritz; R A Jones; R G Lees; H G Khorana
Journal:  Biochemistry       Date:  1978-04-04       Impact factor: 3.162

9.  Preparation of polydeoxynucleotides linked to a solid support by coupling CNBr-activated cellulose with 5'-NH2-terminated oligo and poly(pdT)'s.

Authors:  L Clerici; F Campagnari; J F de Rooij; J H van Boom
Journal:  Nucleic Acids Res       Date:  1979-01       Impact factor: 16.971

10.  Chemical modification of N6-(N-threonylcarbonyl) adenosine. Part II. Condensation of the carboxyl group with amines.

Authors:  W J Krzyzosiak; J Biernat; J Ciesiołka; P Górnicki; M Wiewiórowski
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971
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  14 in total

1.  A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays.

Authors:  J Van Ness; S Kalbfleisch; C R Petrie; M W Reed; J C Tabone; N M Vermeulen
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

Review 2.  Diamond and biology.

Authors:  Christoph E Nebel; Dongchan Shin; Bohuslav Rezek; Norio Tokuda; Hiroshi Uetsuka; Hideyuki Watanabe
Journal:  J R Soc Interface       Date:  2007-06-22       Impact factor: 4.118

3.  Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ.

Authors:  U Maskos; E M Southern
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

4.  Effect of the higher-order structure of tRNAs on the stability of hybrids with oligodeoxyribonucleotides: separation of tRNA by an efficient solution hybridization.

Authors:  Y Kumazawa; T Yokogawa; H Tsurui; K Miura; K Watanabe
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

5.  Haplotype phasing of whole human genomes using bead-based barcode partitioning in a single tube.

Authors:  Fan Zhang; Lena Christiansen; Jerushah Thomas; Dmitry Pokholok; Ros Jackson; Natalie Morrell; Yannan Zhao; Melissa Wiley; Emily Welch; Erich Jaeger; Ana Granat; Steven J Norberg; Aaron Halpern; Maria C Rogert; Mostafa Ronaghi; Jay Shendure; Niall Gormley; Kevin L Gunderson; Frank J Steemers
Journal:  Nat Biotechnol       Date:  2017-06-26       Impact factor: 54.908

6.  Advances in Bioconjugation.

Authors:  Jeet Kalia; Ronald T Raines
Journal:  Curr Org Chem       Date:  2010-01       Impact factor: 2.180

7.  Rapid hybridization kinetics of DNA attached to submicron latex particles.

Authors:  S F Wolf; L Haines; J Fisch; J N Kremsky; J P Dougherty; K Jacobs
Journal:  Nucleic Acids Res       Date:  1987-04-10       Impact factor: 16.971

8.  Attachment of benzaldehyde-modified oligodeoxynucleotide probes to semicarbazide-coated glass.

Authors:  M A Podyminogin; E A Lukhtanov; M W Reed
Journal:  Nucleic Acids Res       Date:  2001-12-15       Impact factor: 16.971

9.  Covalent attachment of oligodeoxyribonucleotides to amine-modified Si (001) surfaces.

Authors:  T Strother; R J Hamers; L M Smith
Journal:  Nucleic Acids Res       Date:  2000-09-15       Impact factor: 16.971

10.  Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds.

Authors:  B C Chu; L E Orgel
Journal:  Nucleic Acids Res       Date:  1988-05-11       Impact factor: 16.971
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