Literature DB >> 2062652

A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays.

J Van Ness1, S Kalbfleisch, C R Petrie, M W Reed, J C Tabone, N M Vermeulen.   

Abstract

A procedure for immobilization of well-defined quantities of oligodeoxyribonucleotides (ODNs) to a versatile nylon support is described. The solid support, a nylon-6/6 bead, is covalently coated with poly(ethyleneimine) to provide a reactive spacer-arm for attachment of ODNs. 5'-Aminohexyl-tailed ODNs are selectively activated using 2,4,6-trichloro-1,3,5-triazine (cyanuric chloride) and then covalently attached to the bead via the triazine moiety. The modified nylon support has a low level of binding of nonspecific nucleic acid and efficiently captures both RNA and DNA targets.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 2062652      PMCID： PMC328333          DOI： 10.1093/nar/19.12.3345

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  26 in total

1.  The use of immobilised derivatives of urease and urate oxidase in automated analysis.

Authors:  H Filippusson; W E. Hornby; A McDonald
Journal:  FEBS Lett       Date:  1972-02-15       Impact factor: 4.124

Review 2.  Chemical modifications of proteins: history and applications.

Authors:  G E Means; R E Feeney
Journal:  Bioconjug Chem       Date:  1990 Jan-Feb       Impact factor: 4.774

3.  CCA paper: a new two-dimensional cyanuric chloride-activated matrix for universal application in molecular biology.

Authors:  H D Hunger; C Coutelle; G Behrendt; C Flachmeier; A Rosenthal; A Speer; H Breter; R Szargan; P Franke; J Stahl
Journal:  Anal Biochem       Date:  1986-08-01       Impact factor: 3.365

Review 4.  Conjugates of oligonucleotides and modified oligonucleotides: a review of their synthesis and properties.

Authors:  J Goodchild
Journal:  Bioconjug Chem       Date:  1990 May-Jun       Impact factor: 4.774

Review 5.  Analytical strategies for the use of DNA probes.

Authors:  J A Matthews; L J Kricka
Journal:  Anal Biochem       Date:  1988-02-15       Impact factor: 3.365

Review 6.  Fluoroimmunoassays and immunofluorometric assays.

Authors:  I Hemmilä
Journal:  Clin Chem       Date:  1985-03       Impact factor: 8.327

7.  New methods for the preparation of biospecific adsorbents and immobilized enzymes utilizing trichloro-s-triazine.

Authors:  T H Finlay; V Troll; M Levy; A J Johnson; L T Hodgins
Journal:  Anal Biochem       Date:  1978-06-15       Impact factor: 3.365

8.  Preparation of some immobilized linked enzyme systems and their use in the automated determination of disaccharides.

Authors:  D J Inman; W E Hornby
Journal:  Biochem J       Date:  1974-01       Impact factor: 3.857

9.  Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus.

Authors:  J N Kremsky; J L Wooters; J P Dougherty; R E Meyers; M Collins; E L Brown
Journal:  Nucleic Acids Res       Date:  1987-04-10       Impact factor: 16.971

10.  Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples.

Authors:  M Ranki; A Palva; M Virtanen; M Laaksonen; H Söderlund
Journal:  Gene       Date:  1983 Jan-Feb       Impact factor: 3.688
View more
  10 in total

1.  Depletion of pre-16S rRNA in starved Escherichia coli cells.

Authors:  G A Cangelosi; W H Brabant
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

2.  Materiomics for Oral Disease Diagnostics and Personal Health Monitoring: Designer Biomaterials for the Next Generation Biomarkers.

Authors:  Wenjun Zhang; Ming L Wang; Sammy Khalili; Steven W Cranford
Journal:  OMICS       Date:  2016-01

3.  Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ.

Authors:  U Maskos; E M Southern
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

4.  Attachment of benzaldehyde-modified oligodeoxynucleotide probes to semicarbazide-coated glass.

Authors:  M A Podyminogin; E A Lukhtanov; M W Reed
Journal:  Nucleic Acids Res       Date:  2001-12-15       Impact factor: 16.971

5.  Development of PCR-based hybridization protocol for identification of streptococcal species.

Authors:  R W Bentley; J A Leigh
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

6.  The use of oligodeoxynucleotide probes in chaotrope-based hybridization solutions.

Authors:  J Van Ness; L Chen
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971

7.  Functionalization of poly(methyl methacrylate) (PMMA) as a substrate for DNA microarrays.

Authors:  F Fixe; M Dufva; P Telleman; C B V Christensen
Journal:  Nucleic Acids Res       Date:  2004-01-12       Impact factor: 16.971

8.  Preparation of a DNA matrix via an electrochemically directed copolymerization of pyrrole and oligonucleotides bearing a pyrrole group.

Authors:  T Livache; A Roget; E Dejean; C Barthet; G Bidan; R Téoule
Journal:  Nucleic Acids Res       Date:  1994-08-11       Impact factor: 16.971

9.  Development of a single-step subtraction method for eukaryotic 18S and 28S ribonucleic acids.

Authors:  Marie J Archer; Baochuan Lin
Journal:  J Biomed Biotechnol       Date:  2011-06-25

10.  Structure-Based Design and Synthesis of a New Phenylboronic-Modified Affinity Medium for Metalloprotease Purification.

Authors:  Shangyong Li; Linna Wang; Ximing Xu; Shengxiang Lin; Yuejun Wang; Jianhua Hao; Mi Sun
Journal:  Mar Drugs       Date:  2016-12-27       Impact factor: 5.118
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.