Literature DB >> 3748808

Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides.

S Agrawal, C Christodoulou, M J Gait.   

Abstract

The syntheses are described of two types of linker molecule useful for the specific attachment of non-radioactive labels such as biotin and fluorophores to the 5' terminus of synthetic oligodeoxyribonucleotides. The linkers are designed such that they can be coupled to the oligonucleotide as a final step in solid-phase synthesis using commercial DNA synthesis machines. Increased sensitivity of biotin detection was possible using an anti-biotin hybridoma/peroxidase detection system.

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Year:  1986        PMID： 3748808      PMCID： PMC311633          DOI： 10.1093/nar/14.15.6227

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  25 in total

1.  PARTIAL PURIFICATION OF SOLUBLE RNA.

Authors:  P C Zamecnik; M L Stephenson; J F Scott
Journal:  Proc Natl Acad Sci U S A       Date:  1960-06       Impact factor: 11.205

2.  The synthesis of oligoribonucleotides. 3. Monoacylation of ribonucleosides and derivatives via orthoester exchange.

Authors:  H P Fromageot; B E Griffin; C B Reese; J E Sulston
Journal:  Tetrahedron       Date:  1967-05       Impact factor: 2.457

3.  Identification of N,N-dimethyltryptamine, 5-methoxy-N,N-dimethyltryptamine and bufotenin by cellulose TLC.

Authors:  P Baumann
Journal:  J Chromatogr       Date:  1973-11-07

4.  The avidin-biotin complex in affinity cytochemistry.

Authors:  E A Bayer; E Skutelsky; M Wilchek
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

5.  Use of ribonucleosides as protecting groups in synthesis of polynucleotides with phosphorylated terminals.

Authors:  J G Nadeau; C K Singleton; G B Kelly; H L Weith; G R Gough
Journal:  Biochemistry       Date:  1984-12-04       Impact factor: 3.162

6.  Detection of specific DNA sequences with short biotin-labeled probes.

Authors:  B C Chu; L E Orgel
Journal:  DNA       Date:  1985-08

7.  Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.

Authors:  T Kempe; W I Sundquist; F Chow; S L Hu
Journal:  Nucleic Acids Res       Date:  1985-01-11       Impact factor: 16.971

8.  Irreversible inhibition of biotin transport in yeast by biotinyl-p-nitrophenyl ester.

Authors:  J M Becker; M Wilchek; E Katchalski
Journal:  Proc Natl Acad Sci U S A       Date:  1971-10       Impact factor: 11.205

9.  Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.

Authors:  N D Sinha; J Biernat; J McManus; H Köster
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

10.  Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

Authors:  M L Duckworth; M J Gait; P Goelet; G F Hong; M Singh; R C Titmas
Journal:  Nucleic Acids Res       Date:  1981-04-10       Impact factor: 16.971
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  46 in total

1.  The estimation of distances between specific backbone-labeled sites in DNA using fluorescence resonance energy transfer.

Authors:  H Ozaki; L W McLaughlin
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

2.  Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridization histochemistry.

Authors:  A Dagerlind; K Friberg; A J Bean; T Hökfelt
Journal:  Histochemistry       Date:  1992-08

3.  Incorporation of multiple reporter groups on synthetic oligonucleotides.

Authors:  J Y Tang; S Agrawal
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

4.  The preparation and application of functionalised synthetic oligonucleotides: III. Use of H-phosphonate derivatives of protected amino-hexanol and mercapto-propanol or -hexanol.

Authors:  N D Sinha; R M Cook
Journal:  Nucleic Acids Res       Date:  1988-03-25       Impact factor: 16.971

5.  Localization of pre-messenger RNA at discrete nuclear sites.

Authors:  J Wang; L G Cao; Y L Wang; T Pederson
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-15       Impact factor: 11.205

6.  The synthesis of protected 5'-amino-2',5'-dideoxyribonucleoside-3'-O-phosphoramidites; applications of 5'-amino-oligodeoxyribonucleotides.

Authors:  B S Sproat; B Beijer; P Rider
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

7.  Synthesis of oligodeoxyribonucleotide with aliphatic amino or phosphate group at the 5' end by the phosphotriester method on a polystyrene support.

Authors:  T Tanaka; T Sakata; K Fujimoto; M Ikehara
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

8.  Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups.

Authors:  J Grzybowski; D W Will; R E Randall; C A Smith; T Brown
Journal:  Nucleic Acids Res       Date:  1993-04-25       Impact factor: 16.971

9.  Oligonucleotides, part 5+: synthesis and fluorescence studies of DNA oligomers d(AT)5 containing adenines covalently linked at C-8 with dansyl fluorophore.

Authors:  D Singh; V Kumar; K N Ganesh
Journal:  Nucleic Acids Res       Date:  1990-06-11       Impact factor: 16.971

10.  Synthesis and hybridization of a series of biotinylated oligonucleotides.

Authors:  A F Cook; E Vuocolo; C L Brakel
Journal:  Nucleic Acids Res       Date:  1988-05-11       Impact factor: 16.971
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