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Literature DB >> 424291

Preparation of polydeoxynucleotides linked to a solid support by coupling CNBr-activated cellulose with 5'-NH2-terminated oligo and poly(pdT)'s.

L Clerici, F Campagnari, J F de Rooij, J H van Boom.

Abstract

Synthetically-prepared 5'-NH2-dT(pdT)n oligomers (66,n=4 or 7) were immobilized on cyanogen bromide activated cellulose. The influence of temperature, pH, and ionic strength on the rate of the coupling process was studied. The oligomer 5'-NH2-DT(pdT)8 could be elongated enzymatically to the polymers 5'-NH2-dT(pdT)n (n=20, 51 and 84), which could be immobilized on cellulose. The cellulose-NH-dT(pdT)84 polymer thus obtained could be assembled to a new solid-state polymer e.g. poly(dA)290 . poly(/3H/dT)200, poly(dT)85-cellulose which, in turn, was a very convenient substrate for assaying DNA-ligase.

Entities: Chemical

Mesh：

Substances：

Year: 1979 PMID： 424291 PMCID： PMC327686 DOI： 10.1093/nar/6.1.247

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

1. An improved method for the insolubilization of DNA for affinity chromatography.

Authors: D Rickwood
Journal: Biochim Biophys Acta Date: 1972-04-26

2. Chemical linkage of nucleic acids to neutral and phosphorylated cellulose powders and isolation of specific sequences by affinity chromatography.

Authors: T Y Shih; M A Martin
Journal: Biochemistry Date: 1974-07-30 Impact factor: 3.162

3. Studies on polynucleotides. The linkage of deoxyribopolynucleotide templates to cellulose and its use in their replication.

Authors: A Panet; H G Khorana
Journal: J Biol Chem Date: 1974-08-25 Impact factor: 5.157

2. Assessment of methods for covalent binding of nucleic acids to magnetic beads, Dynabeads, and the characteristics of the bound nucleic acids in hybridization reactions.

Authors: V Lund; R Schmid; D Rickwood; E Hornes
Journal: Nucleic Acids Res Date: 1988-11-25 Impact factor: 16.971

Review 3. An RNA-centric historical narrative around the Protein Data Bank.

Authors: Eric Westhof; Neocles B Leontis
Journal: J Biol Chem Date: 2021-03-18 Impact factor: 5.157

3 in total

Preparation of polydeoxynucleotides linked to a solid support by coupling CNBr-activated cellulose with 5'-NH2-terminated oligo and poly(pdT)'s.

1. An improved method for the insolubilization of DNA for affinity chromatography.

2. Chemical linkage of nucleic acids to neutral and phosphorylated cellulose powders and isolation of specific sequences by affinity chromatography.

3. Studies on polynucleotides. The linkage of deoxyribopolynucleotide templates to cellulose and its use in their replication.

4. [New synthesis of nucleotide analogues of 5'-amino-5'-desoxy-nucleosides].

5. Purification of a DNA polymerase from calf thymus nuclei.

6. Polynucleotide celluloses as solid state primers and templates for polymerases.

7. Covalent attachment of nucleic acids to agarose for affinity chromatography.

8. Deoxynucleotide-polymerizing enzymes of calf thymus gland. V. Homogeneous terminal deoxynucleotidyl transferase.

9. Covalent attachment of DNA to agarose. Improved synthesis and use in affinity chromatography.

10. The activity of mammalian polynucleotide ligase on x-irradiated DNAs.

1. Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus.

2. Assessment of methods for covalent binding of nucleic acids to magnetic beads, Dynabeads, and the characteristics of the bound nucleic acids in hybridization reactions.

Review 3. An RNA-centric historical narrative around the Protein Data Bank.