Literature DB >> 3548707

Purification, N-terminal amino acid sequence and properties of hydroxymethylbilane synthase (porphobilinogen deaminase) from Escherichia coli.

G J Hart, C Abell, A R Battersby.   

Abstract

Hydroxymethylbilane synthase (porphobilinogen deaminase) was purified to apparent homogeneity from Escherichia coli. The enzyme is a monomer of Mr approx. 40,000. The Km for porphobilinogen and relative Vmax. values have been obtained at various pH values over the range 6.2-8.8, enabling pK values for ionizable groups important for activity to be determined. The N-terminal amino acid sequence is presented.

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Year:  1986        PMID： 3548707      PMCID： PMC1147405          DOI： 10.1042/bj2400273

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

1.  The purification and properties of uroporphyrinogen I synthases and uroporphyrinogen III cosynthase. Interactions between the enzymes.

Authors:  M Higuchi; L Bogorad
Journal:  Ann N Y Acad Sci       Date:  1975-04-15       Impact factor: 5.691

2.  Purification and properties of uroporphyrinogen I synthetase from Rhodopseudomonas spheroides.

Authors:  P M Jordan; D Shemin
Journal:  J Biol Chem       Date:  1973-02-10       Impact factor: 5.157

3.  Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.

Authors:  K Weber; J R Pringle; M Osborn
Journal:  Methods Enzymol       Date:  1972       Impact factor: 1.600

4.  The isolation and characterization of catalytically competent porphobilinogen deaminase-intermediate complexes.

Authors:  A Berry; P M Jordan; J S Seehra
Journal:  FEBS Lett       Date:  1981-07-06       Impact factor: 4.124

5.  Purification and properties of uroporphyrinogen I synthase from human erythrocytes. Identification of stable enzyme-substrate intermediates.

Authors:  P M Anderson; R J Desnick
Journal:  J Biol Chem       Date:  1980-03-10       Impact factor: 5.157

6.  Purification by affinity chromatography and properties of uroporphyrinogen I synthetase from Chlorella regularis.

Authors:  Y Shioi; M Nagamine; M Kuroki; T Sasa
Journal:  Biochim Biophys Acta       Date:  1980-12-04

7.  Modification of hydroxymethylbilane synthase (porphobilinogen deaminase) by pyridoxal 5'-phosphate. Demonstration of an essential lysine residue.

Authors:  G J Hart; F J Leeper; A R Battersby
Journal:  Biochem J       Date:  1984-08-15       Impact factor: 3.857

8.  Characterization of the multiple forms of hydroxymethylbilane synthase from rat spleen.

Authors:  D C Williams
Journal:  Biochem J       Date:  1984-02-01       Impact factor: 3.857

9.  Purification of porphobilinogen deaminase from Euglena gracilis and studies of its kinetics.

Authors:  D C Williams; G S Morgan; E McDonald; A R Battersby
Journal:  Biochem J       Date:  1981-01-01       Impact factor: 3.857

10.  Polypyrroles formed from porphobilinogen and amines by uroporphyrinogen synthetase of Rhodopseudomonas spheroides.

Authors:  R C Davies; A Neuberger
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857
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  10 in total

1.  Evidence that the pyrromethane cofactor of hydroxymethylbilane synthase (porphobilinogen deaminase) is bound through the sulphur atom of a cysteine residue.

Authors:  G J Hart; A D Miller; A R Battersby
Journal:  Biochem J       Date:  1988-06-15       Impact factor: 3.857

2.  Evidence that the pyrromethane cofactor of hydroxymethylbilane synthase (porphobilinogen deaminase) is bound to the protein through the sulphur atom of cysteine-242.

Authors:  A D Miller; G J Hart; L C Packman; A R Battersby
Journal:  Biochem J       Date:  1988-09-15       Impact factor: 3.857

3.  Purification, crystallization and properties of porphobilinogen deaminase from a recombinant strain of Escherichia coli K12.

Authors:  P M Jordan; S D Thomas; M J Warren
Journal:  Biochem J       Date:  1988-09-01       Impact factor: 3.857

4.  Investigation of putative active-site lysine residues in hydroxymethylbilane synthase. Preparation and characterization of mutants in which (a) Lys-55, (b) Lys-59 and (c) both Lys-55 and Lys-59 have been replaced by glutamine.

Authors:  A Hädener; P R Alefounder; G J Hart; C Abell; A R Battersby
Journal:  Biochem J       Date:  1990-10-15       Impact factor: 3.857

5.  Studies on the mechanism of hydroxymethylbilane synthase concerning the role of arginine residues in substrate binding.

Authors:  M Lander; A R Pitt; P R Alefounder; D Bardy; C Abell; A R Battersby
Journal:  Biochem J       Date:  1991-04-15       Impact factor: 3.857

6.  Evidence that pyridoxal phosphate modification of lysine residues (Lys-55 and Lys-59) causes inactivation of hydroxymethylbilane synthase (porphobilinogen deaminase).

Authors:  A D Miller; L C Packman; G J Hart; P R Alefounder; C Abell; A R Battersby
Journal:  Biochem J       Date:  1989-08-15       Impact factor: 3.857

7.  Purification and properties of porphobilinogen deaminase from Arabidopsis thaliana.

Authors:  R M Jones; P M Jordan
Journal:  Biochem J       Date:  1994-05-01       Impact factor: 3.857

8.  Biosynthesis of vitamin B12: the preparative multi-enzyme synthesis of precorrin-3A and 20-methylsirohydrochlorin (a 2,7,20-trimethylisobacteriochlorin).

Authors:  N P Stamford; J Crouzet; B Cameron; A I Alanine; A R Pitt; A A Yeliseev; A R Battersby
Journal:  Biochem J       Date:  1996-01-01       Impact factor: 3.857

9.  Structure and expression of chloroplast-localized porphobilinogen deaminase from pea (Pisum sativum L.) isolated by redundant polymerase chain reaction.

Authors:  M Witty; A D Wallace-Cook; H Albrecht; A J Spano; H Michel; J Shabanowitz; D F Hunt; M P Timko; A G Smith
Journal:  Plant Physiol       Date:  1993-09       Impact factor: 8.340

10.  The common origins of the pigments of life-early steps of chlorophyll biosynthesis.

Authors:  Y J Avissar; P A Moberg
Journal:  Photosynth Res       Date:  1995-06       Impact factor: 3.573
  10 in total

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